Enzyme activation by alternating magnetic field: Importance of the bioconjugation methodology

Armenia, Ilaria; Bonavia, Maria Valeria Grazu; Matteis, Laura De; Ivanchenko, Pavlo; Martra, Gianmario; Gornati, Rosalba; Fuente, Jesús M. de la; Bernardini, Giovanni

doi:10.1016/j.jcis.2018.11.058

Cited by 55 publications

(46 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Activity control via a change in the (micro) temperature by means of enzyme immobilization on magnetic nanoparticles (ferromagnetic or superparamagnetic) or plasmonic gold nanoparticles are other potential methods for enzyme activity control. For immobilization on magnetic particles, the application/removal of an external alternating magnetic field leads to subsequent changes in the micro temperature around the magnetic beads; for immobilization on plasmonic gold nanoparticles irradiation leads to changes in the micro temperature (Figure ).…”

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

“…and Armenia et al. showed that the micro temperatures around the superparamagnetic particles could be varied between 25 °C and 90 °C, aiming for optimal reaction conditions for thermophilic enzymes, while the reaction medium temperature remained almost constant . To demonstrate the feasibility of the system for activating thermophilic enzymes, Armenia et al.…”

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

“…To demonstrate the feasibility of the system for activating thermophilic enzymes, Armenia et al. conjugated α‐amylase from Bacillus licheniformis and l ‐aspartate oxidase from Solfolobus tokodaii to iron oxide nanoparticles . By applying an external alternating magnetic field, they successfully, selectively, and remotely activated the enzymes with an increase in micro temperature …”

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

See 2 more Smart Citations

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

2019

View full text Add to dashboard Cite

Multi‐step biocatalytic reactions have gained increasing importance in recent years because the combination of different enzymes enables the synthesis of a broad variety of industrially relevant products. However, the more enzymes combined, the more crucial it is to avoid cross‐reactivity in these cascade reactions and thus achieve high product yields and high purities. The selective control of enzyme activity, i.e., remote on‐/off‐switching of enzymes, might be a suitable tool to avoid the formation of unwanted by‐products in multi‐enzyme reactions. This review compiles a range of methods that are known to modulate enzyme activity in a stimulus‐responsive manner. It focuses predominantly on in vitro systems and is subdivided into reversible and irreversible enzyme activity control. Furthermore, a discussion section provides indications as to which factors should be considered when designing and choosing activity control systems for biocatalysis. Finally, an outlook is given regarding the future prospects of the field.

show abstract

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

Section: Methods For the Regulation Of Enzyme Activitymentioning

confidence: 99%

See 1 more Smart Citation

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

2019

View full text Add to dashboard Cite

show abstract

“…Ion exchange resins are materials widely used in immobilization due to their good capabilities such as uniform pore diameter, porosity, and high surface area [37]. In this context, Fe 3 O 4 magnetic nanoparticles have been used as the carrier thanks to high surface-volume ratio, their unique magnetic properties, and easy separation by external magnetic field [38,39]. Therefore, ST0779 was immobilized on SiO 2 -Fe 3 O 4 -NP and ST2570 was immobilized on the styrene anion exchange resin (D380).…”

Section: Immobilization Efficiency and Activity Recovery Ratementioning

confidence: 99%

Design and Construction of an Effective Expression System with Aldehyde Tag for Site-Specific Enzyme Immobilization

Wang

Jian

et al. 2020

Catalysts

View full text Add to dashboard Cite

In recent years, the development and application of site-specific immobilization technology for proteins have undergone significant advances, which avoids the unwanted and random covalent linkage between the support and active site of protein in the covalent immobilization. Formylglycine generating enzyme (FGE) can transform the cysteine from a conversed 6-amino-acid sequence CXPXR into formylglycine with an aldehyde group (also termed as “aldehyde tag”). Based on the frame of pET-28a, the His-tags were replaced with aldehyde tags. Afterward, a set of plasmids were constructed for site-specific covalent immobilization, their His-tags were knock out (DH), or were replaced at different positions: N-terminal (NQ), C-terminal (CQ), or both (DQ) respectively. Three different enzymes, thermophilic acyl aminopeptidase (EC 3.4.19.1) from Sulfolobus tokodaii (ST0779), thermophilic dehalogenase (EC 3.8.1.2) from Sulfolobus tokodaii (ST2570), and Lipase A (EC 3.1.1.3) from Bacillus subtilis (BsLA) were chosen as model enzymes to connect with these plasmid systems. The results showed that different aldehyde-tagged enzymes can be successfully covalently attached to different carriers modified with an amino group, proving the universality of the method. The new immobilized enzyme also presented better thermostability and reutilization than those of the free enzyme.

show abstract

“…Among nanoscale iron, superparamagnetic iron oxide NPs are widely studied for magnetic resonance imaging (MRI), for cancer treatment (Song et al 2019) and for targeting antibiotics (Armenia et al 2018), enzymes (Balzaretti et al 2017) and other drugs (Moros et al 2018). In addition, iron NPs functionalized with thermophilic enzymes might find their place also in industrial biotechnology (Armenia et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

et al. 2019

Self Cite

View full text Add to dashboard Cite

Iron nanoparticles (NPs) have been proposed as a tool in very different fields such as environmental remediation and biomedical applications, including food fortification against iron deficiency, even if there is still concern about their safety. Here, we propose Xenopus laevis embryos as a suitable model to investigate the toxicity and the bio-interactions at the intestinal barrier of Fe 3 O 4 and zerovalent iron (ZVI) NPs compared to Fe(II) and (III) salts in the 5 to 100 mg Fe/L concentration range using the Frog Embryo Teratogenesis Assay in Xenopus (FETAX). Our results demonstrated that, at concentrations at which iron salts induce adverse effects, both iron NPs do not cause acute toxicity or teratogenicity even if they accumulate massively in the embryo gut. Prussian blue staining, confocal and electron microscopy allowed mapping of iron NPs in enterocytes, along the paracellular spaces and at the level of the basement membrane of a well-preserved intestinal epithelium. Furthermore, the high bioaccumulation factor and the increase in embryo length after exposure to iron NPs suggest greater iron intake, an essential element for organisms. Together, these results improve the knowledge on the safety of orally ingested iron NPs and their interaction with the intestinal barrier, useful for defining the potential risks associated with their use in food/feed fortification.

show abstract

Enzyme activation by alternating magnetic field: Importance of the bioconjugation methodology

Cited by 55 publications

References 50 publications

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

Design and Construction of an Effective Expression System with Aldehyde Tag for Site-Specific Enzyme Immobilization

Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

Contact Info

Product

Resources

About