Enzymatically synthesized glycogen reduces lipid accumulation in diet-induced obese rats

Furuyashiki, Takashi; Ogawa, Rui; Nakayama, Yôko; Honda, Kazuhisa; Kamisoyama, Hiroshi; Takata, Hitoshi; Yasuda, Michiko; Kuriki, Takashi; Ashida, Hitoshi

doi:10.1016/j.nutres.2013.06.006

Cited by 16 publications

(21 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that ESG and exercise may suppress abdominal fat accumulation by different mechanisms. There is evidence that dietary ESG significantly increased the amount of lipid in the feces of rats (16). We also confirmed that oral administration of ESG with olive oil significantly suppressed the postprandial elevation of plasma TG in mice (unpublished data).…”

Section: Discussionsupporting

confidence: 82%

“…On the other hand, the mRNA levels of hepatic lipid metabolism-related genes were not changed by ESG (16). We also confirmed that dietary ESG affected neither the mRNA levels of hepatic lipid metabolism-related enzymes nor the mRNA levels of UCP-1 or the protein levels of eNOS in the brown adipose tissue in HFDinduced obese mice, although the weight of abdominal fat was significantly decreased (unpublished data).…”

supporting

confidence: 77%

“…In addition, a number of studies suggest that exercise stimulates fatty acid oxidation in the gastrocnemius muscle of mice (16,(25)(26)(27)(28). Therefore, we finally examined the effects of ESG and exercise on the mRNA levels of lipid metabolism-related genes in the gastrocnemius muscle in HFD-fed mice (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…ESG closely resembles natural source glycogen in structural parameters, physicochemical properties and molecular shape (11)(12)(13) and shows various biological properties, including immunostimulating activity (14), indigestibility (15) and intestinal environment improving effect (15). Moreover, the addition of ESG in a HFD resulted in significant decreases in total body fat mass as well as visceral fat mass in rats (16). Dietary ESG significantly increased the amount of lipid in the feces in rats (16).…”

mentioning

confidence: 90%

“…Moreover, the addition of ESG in a HFD resulted in significant decreases in total body fat mass as well as visceral fat mass in rats (16). Dietary ESG significantly increased the amount of lipid in the feces in rats (16). We previously showed that about 20% of ingested ESG acts as a dietary fiber (15), and dietary fiber was reported to increase the amount of fecal lipid (17,18).…”

mentioning

confidence: 96%

See 4 more Smart Citations

Effects of Enzymatically Synthesized Glycogen and Exercise on Abdominal Fat Accumulation in High-Fat Diet-Fed Mice

Tamura

Honda

Morinaga

et al. 2017

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

SummaryThe combination of diet and exercise is the first choice for the treatment of obesity and metabolic syndrome. We previously reported that enzymatically synthesized glycogen (ESG) suppresses abdominal fat accumulation in obese rats. However, the effect of the combination of ESG and exercise on abdominal fat accumulation has not yet been investigated. Our goal in this study was therefore to evaluate the effects of dietary ESG and its combination with exercise on abdominal fat accumulation in high-fat diet (HFD)-fed mice. Male ICR mice were assigned to four groups: HFD, HFD containing 20% ESG, HFD with exercise, HFD containing 20% ESG with exercise. Treadmill exercise was performed for 3 wk (25 m/ min, 30 min/d, 3 d/wk) after 5-d adaption to running at that speed. Both ESG and exercise significantly reduced the weights of abdominal adipose tissues. In addition, the combination of ESG and exercise significantly suppressed abdominal fat accumulation, suggesting that ESG and exercise showed an additive effect. Exercise significantly increased the mRNA levels of lipid metabolism-related genes such as lipoprotein lipase, peroxisome proliferator-activated receptor delta; factor-delta (PPARd), carnitin palmitoyltransferase b, adipose triglyceride lipase (ATGL), and uncoupling protein-3 in the gastrocnemius muscle. On the other hand, dietary ESG significantly decreased the mRNA levels of PPARd and ATGL in the gastrocnemius muscle. These results suggest that the combined treatment of ESG and exercise effectively suppresses abdominal fat accumulation in HFD-fed mice by different mechanisms.

show abstract

Section: Discussionsupporting

confidence: 82%

supporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 90%

mentioning

confidence: 96%

See 3 more Smart Citations

Effects of Enzymatically Synthesized Glycogen and Exercise on Abdominal Fat Accumulation in High-Fat Diet-Fed Mice

Tamura

Honda

Morinaga

et al. 2017

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

show abstract

Extracellular enzymatically synthesized glycogen promotes osteogenesis by activating osteoblast differentiation via Akt/GSK‐3β signaling pathway

Ida‐Yonemochi

Nakagawa

Takata

et al. 2019

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

Glycogen is the stored form of glucose and plays a major role in energy metabolism. Recently, it has become clear that enzymatically synthesized glycogen (ESG) has biological functions, such as the macrophage‐stimulating activity. This study aimed to evaluate the effect of ESG on osteogenesis. MC3T3‐E1 cells were cultured with ESG, and their cell proliferative activity and osteoblast differentiation were measured. An in vivo study was conducted in which ESG pellets with BMP‐2 were grafted into mouse calvarial defects and histomorphometrically analyzed for the new bone formation. To confirm the effect of ESG on bone growth in vivo, ESG was orally administered to pregnant mice and the femurs of their pups were examined. We observed that ESG stimulated cell proliferation and enhanced messenger RNA expression of osteocalcin and osteopontin in MC3T3‐E1 cells. ESG was taken up by the cells associated with GLUT‐1 and activated the Akt/GSK‐3β pathway. In vivo, the new bone formation in the calvarial defect was significantly accelerated by ESG and the maternal administration of ESG promoted fetal bone growth. In conclusion, ESG stimulates cell proliferation and differentiation of preosteoblasts via the activation of Akt/GSK‐3β signaling and promotes new bone formation in vivo, suggesting that ESG could be a useful stimulant for osteogenesis.

show abstract

One-step synthesis of glycogen-type polysaccharides from maltooctaose and its structural characteristics

Tran

Jeong

et al. 2022

Carbohydrate Polymers

View full text Add to dashboard Cite

Enzymatically synthesized glycogen reduces lipid accumulation in diet-induced obese rats

Cited by 16 publications

References 26 publications

Effects of Enzymatically Synthesized Glycogen and Exercise on Abdominal Fat Accumulation in High-Fat Diet-Fed Mice

Effects of Enzymatically Synthesized Glycogen and Exercise on Abdominal Fat Accumulation in High-Fat Diet-Fed Mice

Extracellular enzymatically synthesized glycogen promotes osteogenesis by activating osteoblast differentiation via Akt/GSK‐3β signaling pathway

One-step synthesis of glycogen-type polysaccharides from maltooctaose and its structural characteristics

Contact Info

Product

Resources

About