Enzymatically crosslinked silk-nanosilicate reinforced hydrogel with dual-lineage bioactivity for osteochondral tissue engineering

Zhang, Wei; Zhang, Yanan; Zhang, Aini; Chen, Ling; Sheng, Renwang; Li, Xiaolong; Yao, Qingqiang; Chen, Jialin

doi:10.1016/j.msec.2021.112215

Cited by 39 publications

(38 citation statements)

References 52 publications

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“…The large amount of phenolic hydroxyl groups in TA form strong hydrogen bonds with water, thereby increasing the hydrophilicity of SF-TA hydrogel [ 46 ]. Such enhanced hydrophilicity of hydrogel is beneficial for the absorption of bone marrow blood in the initial stage of osteochondral defect repair, which may improve the infiltration of BMSCs and growth factors into the hydrogel, and ultimately promote osteochondral regeneration [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

“…Where indicated, 1 ng/ml IL-1β (Genscript, Nanjing, China) or 400 μM H 2 O 2 (Yuanle, Shanghai, China) was added to the media to stimulate cells. Cell viability was measured with the Calcein-AM/PI Double Staining Kit (Dojindo, Japan), and cell proliferation was measured with Cell Counting Kit-8 (CCK8, APExBIO, Houston, TX), as described previously [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…Osteogenic and chondrogenic induction of BMSCs was performed according to our previous protocol [ 5 ]. Positive induction of osteogenesis was confirmed using BCIP/NBT Alkaline Phosphatase (ALP) Color Development Kit (Beyotime, China).…”

Section: Methodsmentioning

confidence: 99%

“…Samples were scanned using a Micro-CT scanner (Bruker SkyScan1176, Billerica, MA), and high-resolution micro-CT images were collected. Reconstruction and analysis of bone volume/total volume (BV/TV), and trabecular numbers (Tb.N) were carried out by concentrically placing a cylindrical region of interest (ROI, diameter: 5 mm, height: 5 mm) in the center of the original defect region with the threshold of 350–1080 for cartilage analysis, and >1080 for bone analysis [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…Physiologically, when an osteochondral defect occurs and the subchondral bone plate is penetrated, a large number of endogenous bone marrow-derived mesenchymal stem cells (BMSCs) are released from the bone marrow and migrate into the chondral and subchondral areas to participate in the self-repair process [ 4 ]. However, the spontaneous repair process following trauma or microfracture surgery often results in the formation of fibrotic tissue, which is mechanically- and functionally-inferior to normal hyaline cartilage and subchondral bone [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

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Section: Methodsmentioning

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Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects

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Healing Clays Structure and Functions

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Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

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et al. 2024

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The creation of functional hydrogels with robust load‐bearing capacity adaptable to complex tissue regeneration remains challenging. Silk fibroin (SF) is a natural biomaterial with excellent mechanical strength and cell adhesion capacity, possessing tremendous potential to solve the aforementioned dilemma. The excellent biodegradability and biocompatibility and the molecular structure with multiple modifiable moieties provide opportunities for the injectability and multifunctionality of hydrogels. Furthermore, the incorporation of other polymers or active ingredients can improve the basic properties, confer biological activities and pharmacological effects, and provide the prerequisites for hydrogels to fulfill specific requirements. Therefore, SF‐based hydrogels are widely applied in tissue repair and regeneration, especially in bone, skin, nerve, liver, myocardium, and cornea, which have effectively addressed the challenges of scaffolds' lack of cell adhesion sites, excessive degradation, as well as the lack of efficient, long‐lasting antimicrobial properties. This review outlines the properties of SF, discusses the types of materials commonly used in the preparation of hydrogels and their characteristics, and describes the current construction methods of SF hydrogels. Simultaneously, recent advancements in applying SF‐based multifunctional hydrogels within biological tissues are explored, with a focus on their role in tissue repair, highlighting the repair mechanism of SF based on bone and skin.

show abstract

Enzymatically crosslinked silk-nanosilicate reinforced hydrogel with dual-lineage bioactivity for osteochondral tissue engineering

Cited by 39 publications

References 52 publications

Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects

Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects

Healing Clays Structure and Functions

Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

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