Enzymatically crosslinked and mechanically tunable silk fibroin/pullulan hydrogels for mesenchymal stem cells delivery

Li, Tao; Song, Xiongbo; Weng, Changmei; Wang, Xin; Wu, Jiangyi; Lee, Sun; Gong, Xiaoyuan; Zeng, Wei‐Nan; Yang, Liu; Chen, Cheng

doi:10.1016/j.ijbiomac.2018.04.046

Cited by 62 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…47 . Among these, horseradish peroxidase is the most frequently used reagent 46,[48][49][50][51] . In a related study, Nicole R Raia et al 52 used horseradish peroxidase to cross-link SF and tyramine-substituted hyaluronic acid (HA) to form the hydrogels.…”

Section: Enzyme Cross-linkingmentioning

confidence: 99%

Functional silk fibroin hydrogels: preparation, properties and applications

2021

View full text Add to dashboard Cite

show abstract

Section: Enzyme Cross-linkingmentioning

confidence: 99%

Functional silk fibroin hydrogels: preparation, properties and applications

2021

View full text Add to dashboard Cite

show abstract

“…Silk fibroin-based hydrogel preloading with cells is fabricated by mixing cells with the aqueous silk fibroin solution. After gelation of the silk hydrogel, cells were encapsulated in the three-dimensional hydrogel. , In this cell-preloading system, silk fibroin-based hydrogel plays a vital role in protection of the chondrogenic cells and provides new options to facilitate cartilage regeneration …”

Section: Silk Fibroin In Cartilage Tissue Engineeringmentioning

confidence: 99%

“…After gelation of the silk hydrogel, cells were encapsulated in the three-dimensional hydrogel. 114,115 In this cell-preloading system, silk fibroin-based hydrogel plays a vital role in protection of the chondrogenic cells and provides new options to facilitate cartilage regeneration. 116 Coaxial electrospinning is a technology that has been developed to fabricate nanofibers with core−shell structure and encapsulate bioactive growth factors into the core.…”

Section: Silk Fibroin In Cartilage Tissue Engineeringmentioning

confidence: 99%

Advanced Silk Fibroin Biomaterials for Cartilage Regeneration

Cheng

Davoudi

Xing

et al. 2018

ACS Biomater. Sci. Eng.

135

View full text Add to dashboard Cite

Silk fibroin is regarded as a natural fibrous protein with tunable mechanical properties, acceptable biocompatibility, and favorable capability of enhancing attachment, proliferation, and differentiation of chondrocytes. These properties make it suitable for the fabrication of scaffolds and a broad range of silk fibroin-based biomaterials for cartilage regenerative therapy, which can heal functional cartilage without scar tissue. It can be used as a single material for making different kinds of scaffolds or as a composite with other types of biomaterials. Together with some growth factors, silk fibroin scaffolds can form a support for the growth and differentiation of seeding cells, such as chondrogenic lineage cells and mesenchymal stem cells. Recent advancements of silk fibroin in cartilage regeneration are summarized in this review. Furthermore, the manufacturing methods of silk fibroin materials and their applications in the regeneration of cartilage are also discussed.

show abstract

“…SF solution was prepared as described in our previous publication. 25 For the fabrication of SF sponges, SF solution (60 mg/mL) in a cylinder mold (diameter, 6 mm; height, 10 mm) was sequentially frozen at 220°C for 6 hours and at 280°C overnight. Then, the SF sponges were freeze-dried, immersed in 75% ethanol for 30 minutes to achieve b-sheet formation, and finally cut by a custom-made hole puncher (1.5 mm, 2 mm, 5 mm) for the following experiments.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Aptamer-Functionalized Bioscaffold Enhances Cartilage Repair by Improving Stem Cell Recruitment in Osteochondral Defects of Rabbit Knees

Wang

Song

et al. 2019

Am J Sports Med

Self Cite

View full text Add to dashboard Cite

Background: Recruitment of endogenous stem cells has been considered an alternative to cell injection/implantation in articular cartilage repair. Purpose: (1) To develop a cartilage tissue-engineering scaffold with clinically available biomaterials and functionalize the scaffold with an aptamer (Apt19s) that specifically recognizes pluripotent stem cells. (2) To determine whether this scaffold could recruit joint-resident mesenchymal stem cells (MSCs) when implanted into an osteochondral defect in a rabbit model and to examine the effects of cartilage regeneration. Study Design: Controlled laboratory study. Methods: The reinforced scaffold was fabricated by embedding a silk fibroin sponge into silk fibroin/hyaluronic acid–tyramine hydrogel and characterized in vitro. A cylindrical osteochondral defect (3.2 mm wide × 4 mm deep) was created in the trochlear grooves of rabbit knees. The rabbits were randomly assigned into 3 groups: Apt19s-functionalized scaffold group, scaffold-only group, and control group. Animals were sacrificed at 6 and 12 weeks after transplantation. Repaired tissues were evaluated via gross examination, histologic examination, and immunohistochemistry. Results: In vitro, this aptamer-functionalized scaffold could recruit bone marrow–derived MSCs and support cell adhesion. In vivo, the aptamer-functionalized scaffold enhanced cell homing in comparison with the aptamer-free scaffold. The aptamer-functionalized scaffold group also exhibited superior cartilage restoration when compared with the scaffold-only group and the control group. Conclusion: The Apt19s-functionalized scaffold exhibited the ability to recruit MSCs both in vitro and in vivo and achieved a better outcome of cartilage repair than the scaffold only or control in an osteochondral defect model. Clinical Relevance: The findings demonstrate a promising strategy of using aptamer-functionalized bioscaffolds for restoration of chondral/osteochondral defects via aptamer-introduced homing of MSCs.

show abstract

Enzymatically crosslinked and mechanically tunable silk fibroin/pullulan hydrogels for mesenchymal stem cells delivery

Cited by 62 publications

References 36 publications

Functional silk fibroin hydrogels: preparation, properties and applications

Functional silk fibroin hydrogels: preparation, properties and applications

Advanced Silk Fibroin Biomaterials for Cartilage Regeneration

Aptamer-Functionalized Bioscaffold Enhances Cartilage Repair by Improving Stem Cell Recruitment in Osteochondral Defects of Rabbit Knees

Contact Info

Product

Resources

About