Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

Wu, Changzhu; Strehmel, Christine; Achazi, Katharina; Chiappisi, Leonardo; Dernedde, Jens; Lensen, Marga C.; Gradzielski, Michael; Ansorge‐Schumacher, Marion B.; Haag, Rainer

doi:10.1021/bm500705x

Cited by 40 publications

(41 citation statements)

References 66 publications

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“…Recently, hyperbranched nanogels for enzyme encapsulation have been reported by C. Wu et al 60 The oxidative crosslinking of hyperbranched polyglycerol (hPG) functionalized with phenol groups hydrogels are successfully catalyzed by horseradish peroxidase. The hydrogel properties can be adjusted by tuning the crosslinking.…”

Section: Nanocarriers Based On Different Hyperbranched Polymer Typesmentioning

confidence: 99%

Dendritic nanocarriers based on hyperbranched polymers

2015

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Hyperbranched polymers are obtained through one-step polymerization reactions and exhibit properties that are very similar to those of perfect dendrimer analogues. Therefore, hyperbranched polymers are a suitable alternative for perfect dendrimers as building blocks for dendritic nanocarrier systems. With regard to using soluble hyperbranched polymers as carrier systems, their flexible chains are a major benefit as they can adopt and compartment guest molecules. Upon encapsulation, the properties of the host decides the fate of the guest, e.g., solubility, but the host can also shield a guest from the environment and protect it, e.g., from degradation and deactivation. With regard to the advantages of using hyperbranched polymers as nanocarrier systems and their scalable synthesis, we will discuss different types of hyperbranched polymers and their application as nanocarrier systems for drugs, dyes, and other guest molecules.

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Section: Nanocarriers Based On Different Hyperbranched Polymer Typesmentioning

confidence: 99%

Dendritic nanocarriers based on hyperbranched polymers

2015

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“…This leads us to the conclusion that the kinetic aspects of alginate cross-linking and initial network formation in our system are entirely controlled by the enzymatic reaction, which is in agreement with the literature on polymeric systems cross-linked by enzymes. 42 Another justification of the delayed growth might come from the experimental complications in capturing the growth process. In our DLS investigation, the light scattering from a solution containing only alginate and laccase revealed a certain dynamic process.…”

Section: Chemistry Of Materialsmentioning

confidence: 99%

Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

et al. 2015

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We report a novel approach to synthesize monodisperse hydrogel nanoparticles that are tunable in size. The distinctive feature of our approach is the use of a multicopper oxidase enzyme, laccase, as both a biocatalyst and template for nanoparticle growth. We utilize the ferroxidase activity of laccase to initiate localized production of iron(III) cations from the oxidation of iron(II) cations. We demonstrate that nanoparticles are formed in a dilute polymer solution of alginate as a result of cross-linking between alginate and enzymatically produced iron(III) cations. Exerting control over the enzymatic reaction allows for nanometer-scale tuning of the hydrogel nanoparticle radii in the range of 30− 100 nm. The nanoparticles and their growth kinetics were characterized via dynamic light scattering, atomic force microscopy, and UV−vis spectroscopy. This finding opens up a new avenue for the synthesis of tunable nanoscale hydrogel particles for biomedical applications.

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“…Polyglycerol dendrimers (PGDs, Fig. 1) are dendrimers comprising glycerol units, which along with hyperbranched polyglycerols (HPGs) have been extensively studied for biomedical applications [19,20,21,22,23,24,25,26,27]. Unlike HPGs, PGDs are branched molecules with defined molecular weights [28] and thus support host-guest interactions in a stoichiometric manner [29,30,31,32].…”

Section: Introductionmentioning

confidence: 99%

Temperature-induced recovery of a bioactive enzyme using polyglycerol dendrimers: correlation between bound water and protein interaction

Ooya

Ogawa

Takeuchi

2018

Journal of Biomaterials Science, Polymer Edition

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Enzyme application has gained importance over the past decade in bioprocess, biomedical, and pharmaceutical fields. We found that polyglycerol dendrimers (PGDs), which are biocompatible molecules, can recover alcohol dehydrogenase (ADH) from aqueous solution under elevated temperature. A low concentration of PGD (5 wt.%) is sufficient for the recovery of high enzymatic activity, although a high concentration (25-75 wt.%) of glycerol is generally required to stabilize ADH. The enzymatic activity of ADH in suspension with PGDs is over 60% but it is only 10% in that with glycerol. The results of osmolarity and spin-lattice relaxation time (T) of water measurements in the presence of PGDs suggest that increased amounts of bound water to PGD molecules trigger aggregation along with the direct interaction with ADH. PGDs therefore represent good potential additives for direct recovery of enzymes from aqueous solutions.

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Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

Cited by 40 publications

References 66 publications

Dendritic nanocarriers based on hyperbranched polymers

Dendritic nanocarriers based on hyperbranched polymers

Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

Temperature-induced recovery of a bioactive enzyme using polyglycerol dendrimers: correlation between bound water and protein interaction

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