Enzymatically catalyzed degradation of poly (thioether-ester) nanoparticles

Hoelscher, Fernanda; Machado, Thiago O.; Oliveira, Débora de; Araújo, Pedro Henrique Hermes de; Sayer, Cláudia

doi:10.1016/j.polymdegradstab.2018.09.007

Cited by 23 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this kind of application, gold, magnetic, or cerium oxide nanoparticles, carbon nanotubes, graphene oxide, or even polymers may be conjugated to the aptamers (Sharma et al 2015). These nanostructured supports have been commonly synthesized and applied (Almeida et al 2017;Valério et al 2017;Chiaradia et al 2018;Hoelscher et al 2018;Maass et al 2019).…”

Section: Aptamers Conjugationmentioning

confidence: 99%

An overview and future prospects on aptamers for food safety

Schmitz

Valério

Oliveira

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Introduction Many bacteria are responsible for infections in humans and plants, being found in vegetables, water, and medical devices. Most bacterial detection methods are time-consuming and take days to give the result. Aptamers are a promising alternative for a quick and reliable measurement technique to detect bacteria present in food products. Selected aptamers are DNA or RNA oligonucleotides that can bind with bacteria or other molecules with affinity and specificity for the target cells by the SELEX or cell-SELEX technique. This method is based on some rounds to remove the non-ligand oligonucleotides, leaving the aptamers specific to bind to the selected bacteria. Compared with conventional methodologies, the detection approach using aptamers is a rapid, low-cost form of analysis. Objective This review summarizes obtention methods and applications of aptamers in the food industry and biotechnology. Besides, different techniques with aptamers are presented, which enable more effective target detection. Conclusion Applications of aptamers as biosensors, or the association of aptamers with nanomaterials, may be employed in analyses by colorimetric, fluorescence, or electrical devices. Additionally, more efficient ways of sample preparation are presented, which can support food safety to provide human health, with a low-cost method for contaminant detection. Key points • Aptamers are promising for detecting contaminants outbreaks. • Studies are needed to identify aptamers for different targets.

show abstract

Section: Aptamers Conjugationmentioning

confidence: 99%

An overview and future prospects on aptamers for food safety

Schmitz

Valério

Oliveira

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…12 However, unlike thiol-ene photopolymers, the share hold by thiol-ene latexes has remained limited, and no commercial product has emerged yet. 13,14 The main examples result from a miniemulsion photopolymerization, [15][16][17][18][19][20][21][22][23][24] and to a less extent, dispersion 25,26 and emulsion 12,27 (photo)polymerizations. Suspension photopolymerization has been also described, [28][29][30][31][32] but this process does not result in polymer colloids (average particle diameter < 1 µm), but in micrometre solid beads (0.1 -10 mm).…”

Section: Introductionmentioning

confidence: 99%

“…The most used process in this field is miniemulsion thiolene polymerization. [15][16][17][18][19][20][21][22][23][24] Involving smaller and therefore less scattering monomer droplets (50 -500 nm) than an emulsion polymerization, it has the added advantage of imparting greater radiation penetration within the reactor. In addition, Please do not adjust margins Please do not adjust margins the desired particle nucleation mode occurs via radical capture into the monomer droplets, subsequently converted into polymer particles.…”

Section: Introductionmentioning

confidence: 99%

Droplet nucleation in miniemulsion thiol–ene step photopolymerization

Vidal

Schmutz

et al. 2021

Polym. Chem.

View full text Add to dashboard Cite

show abstract

“…22,29,30 The synthesis of nanoparticles via thiol-ene miniemulsion polymerization allows the development of different kinds of material that could not be straightforwardly produced through other polymerization techniques, such as waterborne polyesters, some works were able to synthesize high molecular poly(thioetherester) (PTEE) via thiol-ene polymerization in miniemulsion 22,30 ; in addition, thiol-ene polymerization is a versatile technique that can be applied to the polymerization of biobased monomers from vegetable oils containing double bonds that are less reactive toward traditional free radical polymerization. 31,32 Also, in the past few years this click chemistry reaction has drawn attention to its use in the production of biomaterials, especially PTEE which is biodegradable 33,34 and no cytotoxic. 30 Therefore, this work aims to synthesize nanocomposites suitable to biomedical applications by encapsulating MNPs in situ via thiol-ene polymerization in miniemulsion and to utilize the PTEE/MNPs-oleic acid (OA) hybrid nanoparticles for hyperthermia treatment of tumors.…”

Section: Introductionmentioning

confidence: 99%

Superparamagnetic biobased poly(thioether‐ester) via thiol‐ene polymerization in miniemulsion for hyperthermia

Santos

Machado

Santin

et al. 2020

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Thiol-ene polymerization has been pointed out as a promising technique to produce biobased polymers for biomedical applications due to its advantages, including mild conditions and rapid reaction rates without the formation of byproducts. Therefore, in this study different concentrations of magnetic nanoparticles (MNPs) were incorporated in poly(thioether-ester) (PTEE) nanoparticles by thiol-ene miniemulsion polymerization of biobased monomers to form both linear and branched cross-linked polymers. Loading efficiencies up to approximately 95% (thermogravimetric analysis) of the MNPs within the polymer matrix were obtained. In addition, the substitution of the dithiol 1,4-butanedithiol (64.2%) for the tetrathiol PTEMP (95.8%), increased the encapsulation efficiency by about 30%. Hybrid nanoparticles presented average mean diameters between 95 and260 nm with polydispersity index between 0.13 and 0.42 by DLS, negative zeta potentials around −45 mV and superparamagnetic behavior. The hyperthermia assays performed on breast cells (MDA-MB 231) have shown that the cell death was dependent on the exposure time to the AC magnetic field and the reduction in cell viability was approximately 35%. These results demonstrated the production of superparamagnetic PTEE nanoparticles via thiol-ene polymerization and highlight the promising application of these biobased materials for cancer treatment by hyperthermia.

show abstract

Enzymatically catalyzed degradation of poly (thioether-ester) nanoparticles

Cited by 23 publications

References 30 publications

An overview and future prospects on aptamers for food safety

An overview and future prospects on aptamers for food safety

Droplet nucleation in miniemulsion thiol–ene step photopolymerization

Superparamagnetic biobased poly(thioether‐ester) via thiol‐ene polymerization in miniemulsion for hyperthermia

Contact Info

Product

Resources

About