Enzymatically Active Ultrathin Pepsin Membranes

Abstract

Enzymatically active proteins enable efficient and specific cleavage reactions of peptide bonds.Covalent coupling of the enzymes permits immobilization, which in turn reduces autolysis-induced deactivation. Ultrathin pepsin membranes were prepared by facile interfacial polycondensation of pepsin and trimesoyl chloride.T he pepsin membrane allows for simultaneous enzymatic conversion and selective removal of digestion products.T he large water fluxes through the membrane expedite the transport of large molecules through the pepsin layers.T he presented method enables the large-scale production of ultrathin, cross-linked, enzymatically active membranes.

Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms

“…8 Raaijmakers et al prepared an ultrathin active skin layer by interfacial polymerization of pepsin and trimesoyl chloride on a polyacrylonitrile (PAN) membrane. 9 fluoride (PVDF) membrane via hydrophobic adsorption to prepare a membrane-based nanoscale proteolytic reactor. 10 It was also reported that enzyme could be immobilized on different membranes via covalent bonding, such as PVDF and polyamide membrane, 11,12 and their surface properties profoundly influenced the structure, orientation, and activity of the bound enzyme.…”

Biocatalytic membrane: Go far beyond enzyme immobilization

“…Moreover, biocatalytic membrane can control the molecular weight of product by its separation function. For example, Raaijmakers et al.fabricated a biocatalytic UF membrane via facile interfacial polycondensation of pepsin and trimesoyl chloride on a porous support, and the pore size of this membrane could be adjusted by changing trimesoyl chloride concentration in order to obtain the desirable peptide size in the permeate [42]. However, normally the pore size distribution of the membrane is not uniform, resulting in an uneven molecular weight product.…”

“…peptide and oligosaccharide production) because the product molecular weight can be controlled by optimizing permeate flux (i.e. retention time) [42]. However, the product molecular weight distribution largely depends on the membrane properties, concentration polarization, and fouling layer formation.…”

Catalytically Active Hollow Fiber Membranes with Enzyme‐Embedded Metal–Organic Framework Coating

“…(Bio)catalytic active membranes, which are conventional membranes functionalized with (bio)catalysts, combine the catalytic reactions and the separation of reactants and products in one step. Given the immobilization of the (bio)catalysts onto the membranes, catalyst recycling is possible . We envision the immobilization of enzyme‐active ZIF‐8 MOFs onto commercial hollow fiber membranes, combining the benefits of biocatalytic membranes and enzymatic‐active MOFs, allowing continuous enzymatic reaction without the need of MOF separation from the products.…”