Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes

Soybean Peroxidase‐Catalyzed Treatment of Azo Dyes with or without Fe° Pretreatment

“…pH Optimization for Single-Step Process. Enzymatic treatment has proven to be pH dependent (Chiong et al, 2016;Kalsoom et al, 2013, Mazloum et al, 2016; any enzyme has an optimal pH for maximal efficiency, therefore, it is important to determine the optimal value. Figure 1 shows the pH optimization with a constant of H 2 O 2 concentration and sufficient enzyme for stringency as described in the methodology for the single-step process.…”

“…For DB38 at pH 3.6, with 2.5 mM H 2 O 2 , 3% color remaining was achieved with 3.0 U/mL; with a higher concentration of enzyme there was no increase in the color removal percentage. Chiong et al (2016) needed 0.186 U/mL of SBP at pH 5 with 2 mM H 2 O 2 to treat 0.09 mM Methyl Orange dye during a 1-hour reaction (where one unit of enzymatic activity was the amount of enzyme that catalyzes 1.0 lmol of H 2 O 2 per minute at 25 8C and pH 6.0 with guaiacol as substrate). The substrate concentration used by Chiong et al (2016) was 6 to 12 times less than that used in this research, which results in a lower concentration of SBP needed; also, it should be noted, the definition of U/mL was different.…”

“…Chiong et al (2016) needed 0.186 U/mL of SBP at pH 5 with 2 mM H 2 O 2 to treat 0.09 mM Methyl Orange dye during a 1-hour reaction (where one unit of enzymatic activity was the amount of enzyme that catalyzes 1.0 lmol of H 2 O 2 per minute at 25 8C and pH 6.0 with guaiacol as substrate). The substrate concentration used by Chiong et al (2016) was 6 to 12 times less than that used in this research, which results in a lower concentration of SBP needed; also, it should be noted, the definition of U/mL was different. On the other hand, Mazloum (2014), using the same definition of U/mL SBP as used in this research, required SBP concentrations of 0.75 U/mL SBP for 95% color removal of 1 mM COG and 85% color removal of 1 mM AR4.…”

Fibrous material based on a combination of poly(acrylic acid‐co‐hydroxyethyl methacrylate) with iron ions as a heterogeneous Fenton catalyst for dye oxidative decomposition

“…At present, global textile industries consume more than 10,000 tons of dyes each year, and about 10% of those left in effluents are directly discharged into water bodies . Thus, dye wastewater has become one of our main pollutants, and it can induce serious pollution in available water resources . The dyes in wastewater are toxic, carcinogenic, and/or mutagenic compounds that have a high molecular weight, complex composition, and high chroma; additionally, most of them are nonbiodegradable .…”

Textiles

“…Three different agro-waste, wheat bran (WB), wheat straw (WS) and orange peel (OP) were investigated as substrates for solid state cultivation of white rot fungus, Cyathus bulleri and respective culture filtrates were examined for extracellular enzymes such as peroxidases, and laccases. Results indicated that the WB culture filtrate showed maximum decolorization efficiency (70-85%) followed by OP (60-75%) and WS (40-60%) Chiong et al, (2016). investigated the potential use of soybean peroxidase and Luffa acutangula (luffa) peroxidase, extracted from bio-wastes of soybean hulls and luffa skin peels respectively, for enzymatic degradation of azo dye methyl orange from liquid effluents.…”