Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase

Tran, Dang-Thuan; Yeh, Kuei-Ling; Chen, Ching‐Lung; Chang, Jo‐Shu

doi:10.1016/j.biortech.2011.12.145

Cited by 192 publications

(76 citation statements)

References 34 publications

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“…The conversion of crude microalgal oil to biodiesel increased with increasing methanol to oil ratio. This reached a maximum and started to decrease when methanol to oil ratio was further increased [8]. A similar trend was observed in this study (Fig.…”

Section: Effect Of Methanol Amountsupporting

confidence: 90%

See 1 more Smart Citation

In situ biodiesel production from wet Chlorella vulgaris under subcritical condition

Tsigie

Huynh

Ismadji

et al. 2012

Chemical Engineering Journal

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Section: Effect Of Methanol Amountsupporting

confidence: 90%

“…The lipids are then converted into fatty acid alkyl esters. The extraction efficiency depends on factors such as microalgae species, method of cell wall disruption and solvent used for extraction [5][6][7][8]. Cell disruption prior to extraction can increase the amount of extractable oil.…”

Section: Introductionmentioning

confidence: 99%

In situ biodiesel production from wet Chlorella vulgaris under subcritical condition

Tsigie

Huynh

Ismadji

et al. 2012

Chemical Engineering Journal

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“…Replacing the chemical catalysts used in biodiesel production with biocatalysts shall further enhance the environmental acceptability of the process and lipases could play a prominent role in this sector. They catalyse the transesterification of triacylglycerol with short chain alcohols and immobilization of the enzyme is especially advantageous as it enhances stability and permits reusability (5). The enzyme used in this study was the purified lipase derived from Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Palanisamy¹,

Kumaresan²,

Jeyaseelan³

2015

Iran J Biotech

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Background:Lipase is an enzyme with immense application potential. Ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. Enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. Objective: The objectives of the study were to immobilize the lipase from Bacillus sp. PS35 by cross-linking and adsorption onto styrene-divinyl benzene (Sty-Dvb) hydrophobic resin and to comparatively characterize the free and immobilized lipase preparations. The work also aimed to apply the immobilized lipase for catalysing the fatty acid methyl ester (FAME) synthesis from palm oil and optimize the process parameters for maximizing the yield. Materials and Methods: In this study, the purified lipase from Bacillus sp. PS35 was immobilized by adsorption onto styrene-divinyl benzene hydrophobic resin with gluteraldehyde cross-linking. Results: The immobilized enzyme showed better pH and temperature stabilities than the free lipase. Organic solvent stability was also enhanced, with the relative activity in the presence of methanol being shifted from 53% to 81%, thereby facilitating the enzyme's application in fatty acid methyl ester synthesis. It exhibited remarkable storage stability over a 30-day period and after 20 repetitive uses. Cross-linking also reduced enzyme leakage by 49%. The immobilized lipase was then applied for biodiesel production from palm oil. Methanol and oil molar ratio of 5:1, three step methanol additions, and an incubation temperature of 50°C were established to be the ideal conditions favoring the transesterification reaction, resulting in 97% methyl ester yield. Conclusions: These promising results offer scope for further investigation and process scale up, permitting the enzyme's commercial application in a practically feasible and economically agreeable manner.

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“…The upper polar phase was discarded and the lower phase containing the chloroform and microalgal lipid was separated for further use. Chloroform was evaporated in a hood under ambient temperature 20 . The yield of extraction from microalgal oil was 12 .…”

Section: Oil Extractionmentioning

confidence: 99%

Enzymatic Production of Biodiesel from Microalgal Oil using Ethyl Acetate as an Acyl Acceptor

et al. 2015

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Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase

Cited by 192 publications

References 34 publications

In situ biodiesel production from wet Chlorella vulgaris under subcritical condition

In situ biodiesel production from wet Chlorella vulgaris under subcritical condition

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Enzymatic Production of Biodiesel from Microalgal Oil using Ethyl Acetate as an Acyl Acceptor

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