Enzymatic synthesis of structured lipids with behenic acid at the sn-1, 3 positions of triacylglycerols

Abstract

A long chain saturated fatty acid (SFA), behenic acid, is incorporated into the -1, 3 positions of triacylglycerols in palm olein (POo) and high-oleic sunflower oil (HOS) by solvent-free interesterification catalyzed by Lipozyme RM IM. The enzymatic interesterified HOS (EIE-HOS) yielded 76.5% of BOO and BOB as compared to 45.6% in POo (EIE-POo). The-2 position of EIE-HOS displayed 5.3 mol% of SFA which is significantly lower compared to 13.5 mol% in EIE-POo ( < 0.001). The -1, 3 positions of EIE-POo exhibited greater amount of behenic acid (82.0 mol%) in relation to EIE-HOS (64.0 mol%) ( < 0.001). Due to the greater variety of constitutive triacylglycerol, EIE-POo showed greater differences between onset (To) and offset temperature (Tf) in the melting endotherms (76.99 °C) as compared to EIE-HOS (68.65 °C), and may offer more intensive cooling sensation and flavor release.

Characterization of structured lipids produced through interesterification of blends comprising beef tallow, milk, and vegetable oil using infrared spectroscopy

“…This result could be associated with location of fatty acids on glycerol backbone and chain length of fatty acids. SMP of lipids was reduced since the sn‐2 position of mono‐di and triacylglycerol is conserved with high unsaturated and short chain fatty acids (Kok et al, 2018). The fatty acid profiles of the interesterified lipids in this study were in agreement with previous research (Kowalska et al, 2015; Meng et al, 2011).…”

Effects of a novel medium–long–medium‐type structured lipid synthesized using a two‐step enzymatic method on lipid metabolism and obesity protection in experimental mice

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Obesity can significantly increase the risks of diabetes, atherosclerosis, hyperlipidemia, hypertension, and other diseases; therefore, it has emerged as a major public health concern worldwide (Moreira et al, 2017). Obesity is mainly caused by the excessive intake of dietary energy but insufficient calorie consumption, resulting in systematic accumulation of fat in the body (Kok et al, 2018). Obesity can be effectively prevented and controlled by optimizing the type of fats in the diet and developing low-calorie fats.

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Desenvolvimento e caracterização de carreadores lipídicos nanoestruturados formulados com gorduras interesterificadas para a entrega de fistosteróis livres

“…Outro ponto que pode ter contribuído para esse resultado está relacionado a uma melhor solubilidade do FP em maiores proporções de lipídio líquido pela formação de TAGs com pontos de fusão mais baixo após a interesterificação. Além disso, a interesterificação parece ter contribuído de forma positiva para absorvido pelo organismo durante o processo digestivo, sendo uma parte excretada (Kok et al, 2018;. Essa característica direciona este NLC para a aplicação em alimentos com baixo teor calórico.…”