Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids

Gorke, Johnathan T.; Okrasa, Krzysztof; Louwagie, Andrew; Kazlauskas, Romas J.; Srienc, Friedrich

doi:10.1016/j.jbiotec.2007.04.001

Cited by 68 publications

(49 citation statements)

References 29 publications

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“…The conversion was very low in [Emim]COOCF 3 , the single water-miscible of the tested ILs. Higher conversions in water-immiscible than in water-miscible ILs were also reported by other authors for lipase-catalyzed transesterification reactions (Gorke et al, 2007). ILs with BF 4 − anion were more efficient as reaction media than [Bmim]PF 6 , considering both activity and enantioselectivity.…”

Section: Solvent Engineering Of 2-octanol Acylation Reaction Catalyzesupporting

confidence: 74%

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Applications of Ionic Liquids to Increase the Efficiency of Lipase Biocatalysis

Péter¹,

Paul²,

Ursoiu³

2011

Ionic Liquids: Applications and Perspectives

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Section: Solvent Engineering Of 2-octanol Acylation Reaction Catalyzesupporting

confidence: 74%

“…This miscibility seems to be influenced by the ability of their anions to form hydrogen bonds, not by the overall polarity of the molecule. From the series of ILs with the same (1-butyl-3-methylimidazolium) (Gorke et al, 2007). The explanation is probably the stronger hydrogen-bond accepting ability of water-miscible IL's anions.…”

Section: Introductionmentioning

confidence: 99%

Applications of Ionic Liquids to Increase the Efficiency of Lipase Biocatalysis

Péter¹,

Paul²,

Ursoiu³

2011

Ionic Liquids: Applications and Perspectives

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“…In the academic literature, designer solvents such as ionic liquids 12 have been suggested as useful for both PHA extraction as well as a medium for in vitro enzymatic PHA synthesis. 12 Ionic liquids are attractive because they have a low vapor pressure at typical process conditions and have been demonstrated to be environmentally benign. Unfortunately, it has also been demonstrated that small amounts of water contamination combined with the dense ionic matrix of these liquids often results in unwanted hydrolysis of the polymer chains at the extraction temperatures.…”

Section: Introductionmentioning

confidence: 99%

Solubility and degradation of polyhydroxyalkanoate biopolymers in propylene carbonate

2009

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New biobased materials and chemicals require different processing strategies than petroleum-derived commodities. The extraction and recovery of polyhydroxyalkanoate (PHA) biopolymers from the residual cellular biomass is particularly difficult because the polymer is accumulated within the cell. PHAs have low solubility in many classical polymer solvents and are most often dissolved using undesirable chlorinated solvents. The solubility kinetics is greatly diminished when these polymers are highly crystalline. Here, 1,2-propylene carbonate is used to dissolve highly crystalline polyhydroxybutyrate at ambient pressures and moderate temperatures. We have used kinetic studies of the dissolution of the crystalline material to determine the energy barrier for dissolution of the system. Further, the degradation of polyhydroxybutyrate and similarly prepared PHA block copolymers were studied during this extraction process using molecular weight characterization by gel permeation chromatography. Finally, we have used these findings to extract PHA block copolymers from dried biomass at the bench scale.

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“…Almost all polymerization reactions were conducted in organic solvents, such as: toluene [40], 1,4-dioxane [41], isopropyl ether [42], isooctane [43], diphenyl ether [44], cyclohexane [45]. The reaction was also carried out in supercritical carbon dioxide [46,47], ionic liquids [48][49][50], and recently, in aqueous dispersion [51]. The best solvent for eROP with CALB seems to be toluene [52].…”

Section: Linear and Star Ester Oligomersmentioning

confidence: 99%