Enzymatic synthesis of fructooligosaccharides from date by-products using an immobilized crude enzyme preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033

Smaali, Issam; Jazzar, Souhir; Soussi, Asma; Muzard, Murielle; Aubry, Nathalie; Marzouki, M. Néjib

doi:10.1007/s12257-011-0388-9

Cited by 36 publications

(27 citation statements)

References 23 publications

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“…It was extensively reported that the hydrolytic activity of glycosidases, such as α/β‐glucosidases or β‐fructofuranosidases, could be shifted towards synthesis (trasnsglycosylating activity) using a high substrate concentration. Thus, the α‐glucosidase from the yeast Xanthophyllomyces dendrorhous produced IMOS (mainly panose) using 200–525 g l −1 maltose (Fernández‐Arrojo et al , ; Gutierrez‐Alonso et al , ) and β‐fructofuranosidases from Aspergillus awamori or yeast such as Schwanniomyces occidentalis and Saccharomyces cerevisiae synthesized FOS using different sucrose‐rich by‐products from a variety of palm tree dates (the first) or 600 g l −1 sucrose (the last two) (Álvaro‐Benito et al , ; Lafraya et al , ; Smaali et al , ). In fact, sucrose is a relatively cheap and renewable raw material that has already been widely employed in the synthesis of bioactive oligosaccharides (Monsan and Ouarné, ), and among them several hetero‐oligosaccharides.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

García-González

Plou

Cervantes

et al. 2019

Microbial Biotechnology

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Summary Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload‐sucrose reactions. These products were purified and structurally characterized by MS‐ESI and NMR techniques. The reaction mixture included new sugars showing a great variety of glycosidic bonds including α‐(1→1), α‐(1→3) and α‐(1→6) linkages. The main product synthesized was the trisaccharide isomelezitose, whose maximum concentration reached 81 g l−1, the highest amount reported for any unmodified enzyme or microbial extract. In addition, 51 g l−1 of the disaccharide trehalulose was also produced. Both sugars show potential nutraceutical and prebiotic properties. Interestingly, the sugar mixture obtained in the biosynthetic reactions also contained oligosaccharides such as esculose, a rare trisaccharide with no previous NMR structure elucidation, as well as erlose, melezitose and theanderose. All the sugars produced are naturally found in honey. These compounds are of biotechnological interest due to their potential food, cosmeceutical and pharmaceutical applications.

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Section: Introductionmentioning

confidence: 99%

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

García-González

Plou

Cervantes

et al. 2019

Microbial Biotechnology

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“…As the enzymatic treatment results in most cases in a material that is partially depolymerized, the currently existing literature is mostly focused on applications for oligosaccharides or hemicellulosic materials with a low degree of polymerization. These applications range from dietary supplements to hydrogels for the food or medical industry …”

Section: Enzymatic‐assisted Extraction Of Plant Polysaccharides In a mentioning

confidence: 99%

Enzymatic‐assisted extraction and modification of lignocellulosic plant polysaccharides for packaging applications

Martínez‐Abad

Ruthes

Vilaplana

2015

J of Applied Polymer Sci

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On the use of tris(nonylphenyl) phosphite as a chain extender in melt-blended poly(hydroxybutyrate-co-hydroxyvalerate)/ clay nanocomposites: Morphology, thermal stability, and mechanical properties J. González-Ausejo, E. Sánchez-Safont, J. Gámez-Pérez and L. Cabedo, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Characterization of polyhydroxyalkanoate blends incorporating unpurified biosustainably produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) A. Martínez-Abad, L. Cabedo, C. S. S. Oliveira, L. Hilliou, M. Reis and J. M. Lagarón, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Modification of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) properties by reactive blending with a monoterpene derivative L. Pilon and C. Kelly, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) films for food packaging: Physical-chemical ABSTRACT: Plant polysaccharides comprise the main renewable resource available in the biosphere for biomaterial production. However, the recalcitrant and heterogeneous structure of lignocellulosic biomass hinders the effective fractionation and exploitation of the polysaccharide components for the design of carbohydrate-based materials. Carbohydrate-active enzymes constitute a selective and versatile biotechnological tool that can assist during the biomass pretreatment steps to extract and fractionate the polysaccharide macromolecular components. Moreover, this enzymatic toolbox can be as well exploited for the tailored modification of the molecular structure of relatively pure polysaccharide components to achieve customized macroscopic properties. This review critically discusses the potential and challenges of the use of plant lignocellulosic polysaccharides and enzymatic modifications to design and prepare suitable materials for packaging applications in terms of their structure-property relations. Structural factors such as the molar mass and crystallinity of the polysaccharide fractions and functional factors such as water sensitivity and processability of the derived films are critical for the material performance. The global net primary production of carbon in the biosphere is estimated around 10 11 tonnes per year, 1 from which polysaccharides account for approximately 75% of all biomass. In this context, plant biomass represents the main renewable resource for biofuel and materials production, as a future replacement of fossil-based energy and goods. New schemes for the sustainable exploitation of biomass have emerged in the last decades (the "biorefinery" concept), which integrate chemical, mechanical, thermal, and biotechnological conversion processes for the generation of energy, platform chemicals, and bio-based polymeric materials. Different generations of biorefineries have been implemented, depending on the biomass source (e.g., agriculturederived crops and food waste, lignocellulosic biomass, marine feedstock, designer crops) and on the diversity of products that are converted (e.g., bioethanol after fermentation, syngas after thermochemical conversion, fin...

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“…A crude β-fructofuranosidase preparation from Aspergillus awamori NBRC4033 was covalently bound to chitosan by glutaraldehyde linkages and used for FOS synthesis from sucrose [143]. The obtained crude extract, after filtration and centrifugation, was directly used as an enzyme source in the immobilization process.…”

Section: Immobilized Carbohydrases In the Production Of Prebioticsmentioning

confidence: 99%

Potential Applications of Carbohydrases Immobilization in the Food Industry

Contesini

Figueira

Kawaguti

et al. 2013

IJMS

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Carbohydrases find a wide application in industrial processes and products, mainly in the food industry. With these enzymes, it is possible to obtain different types of sugar syrups (viz. glucose, fructose and inverted sugar syrups), prebiotics (viz. galactooligossacharides and fructooligossacharides) and isomaltulose, which is an interesting sweetener substitute for sucrose to improve the sensory properties of juices and wines and to reduce lactose in milk. The most important carbohydrases to accomplish these goals are of microbial origin and include amylases (α-amylases and glucoamylases), invertases, inulinases, galactosidases, glucosidases, fructosyltransferases, pectinases and glucosyltransferases. Yet, for all these processes to be cost-effective for industrial application, a very efficient, simple and cheap immobilization technique is required. Immobilization techniques can involve adsorption, entrapment or covalent bonding of the enzyme into an insoluble support, or carrier-free methods, usually based on the formation of cross-linked enzyme aggregates (CLEAs). They include a broad variety of supports, such as magnetic materials, gums, gels, synthetic polymers and ionic resins. All these techniques present advantages and disadvantages and several parameters must be considered. In this work, the most recent and important studies on the immobilization of carbohydrases with potential application in the food industry are reviewed.

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Enzymatic synthesis of fructooligosaccharides from date by-products using an immobilized crude enzyme preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033

Cited by 36 publications

References 23 publications

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

Enzymatic‐assisted extraction and modification of lignocellulosic plant polysaccharides for packaging applications

Potential Applications of Carbohydrases Immobilization in the Food Industry

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