“…We prepared recombinant human G-EM by expression in Escherichia coli,a sr eportedp reviously. [22] As ap rototypical acceptors ubstrate, we employed af luorescently labeled disaccharide Mana1-2Mana-C 3 H 6 NH-Dansyl (2)i nl ight of our previousG -EMs tudy, [23] with Glca1-3Mana-F (1)a st he glycosyl donor.A si np revious studies, [24][25][26][27][28] the assay was conducted in phosphate buffer (pH 7.0) containing 1, 2 (1/2,1 :4), and recombinant human G-EM (Scheme 1). After 30 min at 37 8C, HPLC analysis of the reaction mixture revealed an ew peak, which was assigneda s3 [23] ( Figure 1A).…”