Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study

Onoja, Emmanuel; Chandren, Sheela; Razak, Fazira Ilyana Abdul; Wahab, Roswanira Abdul

doi:10.1016/j.jtice.2018.05.049

Cited by 41 publications

(29 citation statements)

References 73 publications

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“…The reasons behind this have to do with its well-reported good stereospecificity, high enantioselectivity, and stability in organic solvents, as well as possessing good thermal stability, the capability of catalyzing a plethora of important industrial reactions. Among these reactions, CRL catalyzes several reactions including hydrolysis, transesterification, esterification, and interesterification which require mild optimal working conditions through an energy-saving route [1][2][3][4]. However, like any other forms of free enzymes, the free form of CRL is vulnerable to deactivation under harsh industrial settings [5].…”

Section: ■ Introductionmentioning

confidence: 99%

“…A versatile crosslinker, glutaraldehyde [22] was chosen to covalently bind the primary amino groups of CRL to the surface of OPL-silica support matrix (CRL/G/A/SiO2-M). This homobifunctional crosslinker activates the functional groups of different support matrices by linking the primary amino groups on the support surface with those of the protein molecules to be immobilized [3,4,[22][23][24].…”

Section: ■ Introductionmentioning

confidence: 99%

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Effect of Glutaraldehyde Concentration on Catalytic Efficacy of Candida rugosa Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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Till date, studies that investigated the effect of glutaraldehyde concentration on catalytic efficacy of biocatalyst developed with silica-derived from oil palm leaves (OPL) as support, are unknown. The study presents the preparation of a support consisting of silica extracted from OPL coated over magnetite (G/A/SiO2-M) for the immobilization of Candida rugosa lipase (CRL). Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. Fourier transform infrared (FTIR) spectra and immobilization parameters indicated that covalent bound CRL on functionalized OPL-derived silica-magnetite composite activated with 4% (v/v) glutaraldehyde solution (100 mM, pH 7.0) (CRL/G/A/SiO2-M) and pretreated in toluene, resulted in a protein loading and an immobilization yield of 68.3 mg/g and 74.3%, respectively. The resultant CRL/G/A/SiO2-M biocatalyst which specific activity was 61.9 U/g catalyzed the esterification production of 76.5% butyl butyrate in just 3 h, as confirmed by analyses of the purified ester using FTIR and 1H NMR spectroscopy. Hence, the finding envisages the promising use of G/A/SiO2-M support fabricated from discarded OPL as a carrier for immobilization and activation of CRL, in conjunction to being a good alternative source of renewable silica.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of Candida rugosa Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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“…The study by Ferreira et al [36], indicated that lipase from Geotrichum candidum required 34.50 kJ/mol of activation energy. While lipase from Aspergillus awamori as studied by Mostafa et al [37], required 32.75 kJ/mol activation energy, and 10.54 kJ/mol activation energy was needed by lipase from Candida rugosa as claimed by Onoja et al [38]. From the data, the activation energy was different according to the other kind of lipase.…”

Section: Effect Of Lipase Activity Towards Temperaturementioning

confidence: 88%

“…The value of Vmax represents the ability of the enzyme in utilizing the substrate to form product, while Km is the affinity of substrate towards the enzyme active site. A higher value of Vmax gives the maximum esterification rate of an enzyme [38], while a lower value of Km of an enzyme shows high affinity of the enzyme-substrate complex [37]. Higher Km value indicated that the enzyme does not bind as efficiently with the substrate and Vmax was only reached if the substrate concentration is high enough to saturate the enzyme.…”

Section: Kinetics Of Enzymementioning

confidence: 99%

Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil

Gusniah

Veny

Hamzah

2020

Bull. Chem. React. Eng. Catal.

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Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanuginosus, TLIM, and Candida Antarctica A (recombinant from Aspergillus oryzae), CALA. These lipases were compared based on their activity at different pH (6-10), temperature (30-50 °C), activation energy, and amount of lipase loading for hydrolysis of p-NPA into n-NP. The result indicates that among the lipase used in the study, CALA is the preferable biocatalyst in the hydrolysis of p-NPA due to the minimum energy required and higher enzymatic activity at 20 mg of enzyme loading. PcL and CALA used in the study gave the optimum activity at pH 9 except for TLIM at pH 8 and the optimum temperature at 40 °C. The kinetic data obtained for CALA in this reaction were Km = 57.412 mM and Vm = 70 µM/min. This finding shows that CALA is beneficial biocatalysts for the transesterification process to obtain a higher product with lower activation energy. Copyright © 2020 BCREC Group. All rights reserved

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“…Being the most important agricultural crop in Malaysia, palm oil is the fourth largest contributor to the nation's economy [4]. Nonetheless, biomass from oil palm industries generated from pruning, replanting and milling activities are currently underutilized, as apparent from the insignificant conversions of oil palm biomass into valueadded products [5]. The biomass of the oil palm constitutes oil palm fronds, oil palm trunk, empty fruit bunches, palm kernel shells, mesocarp fibers and palm oil mill effluent [6].…”

Section: ■ Introductionmentioning

confidence: 99%

Statistical Modelling of Ultrasonic-Aided Extraction of Elaeis guineensis Leaves for Better-Quality Yield and Total Phenolic Content

et al. 2019

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The present study highlighted the statistical modeling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time (X2: 5−55 min), the solvent-to-solid ratio (X3: 15:1−35:1 mL/g) and sonification amplitude (X4: 20−100 %). Under optimized conditions, the highest EY of 14.38% was attained using 50% (v/v) ethanol:water ratio, 55 min, 35 mL/g solvent-to-solid ratio, 60% sonication amplitude, whereas maximum TPC was 209.70 mg gallic acid equivalent (GAE)/g [50% (v/v) ethanol:water ratio, 30 min, 25 mL/g solvent-to-solid ratio, 60% sonication amplitude]. Second-order polynomial models of EY and TPC showed the R2 value corresponding to 0.9303 and 0.9500, respectively, indicating their significance (p < 0.05) to predict the responses. HPLC chromatograms revealed gallic acid and catechin were present in the UAE extracts. UAE technique afforded better EY (14.38%) and TPC (209.70 mg GAE/g) than maceration (3.73%, 85.23 mg GAE/g) and Soxhlet (6.86%, 102.13 mg GAE/g) extractions, as based on scanning electron micrographs of untreated, UAE, macerated and Soxhlet treated samples. Cell walls of ultrasonic-treated E. guineensis leaves were visibly disrupted to facilitate the higher release of bioactive plant materials, thus justifying the higher EY and TPC. The application of ultrasound appeared to remarkably increase the extraction efficiency of E. guineensis leaves to extract as compared to the conventional methods.

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Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study

Cited by 41 publications

References 73 publications

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil

Statistical Modelling of Ultrasonic-Aided Extraction of <i>Elaeis guineensis</i> Leaves for Better-Quality Yield and Total Phenolic Content

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