Enzymatic synthesis of 6- and 6′-O-linoleyl-α-d-maltose: From solvent-free to binary ionic liquid reaction media

Effects of Particle Size of Sucrose Suspensions and Pre‐incubation of Enzymes on Lipase‐Catalyzed Synthesis of Sucrose Oleic Acid Esters

“…In contrast, biocatalytic synthesis occurs in mild reaction conditions, thereby reducing the formation of undesirable by-products and lowering energy consumption during processing [12]. In the literature, numerous approaches for lipase-catalyzed reactions have been conducted, including polar solvents [13][14][15], ionic liquids [16][17][18], microwave assistance [19], ultrasound irradiation [20], and supercritical carbon dioxide [21], to overcome the poor miscibility of acyl donors and acceptors reviewed in the publications [22,23].…”

“…In contrast, biocatalytic synthesis occurs in mild reaction conditions, thereby reducing the formation of undesirable by-products and lowering energy consumption during processing [12]. In the literature, numerous approaches for lipase-catalyzed reactions have been conducted, including polar solvents [13][14][15], ionic liquids [16][17][18], microwave assistance [19], ultrasound irradiation [20], and supercritical carbon dioxide [21], to overcome the poor miscibility of acyl donors and acceptors reviewed in the publications [22,23].Recently, saccharide fatty acid esters have been enzymatically synthesized in solvent-free media in our laboratory, by making use of the desired product, monoester, to enhance the miscibility of acyl acceptor and donor, saccharide and fatty acid, respectively [24][25][26][27]. A closedloop bioreactor system operated under continuous recirculation was utilized in the preparation of sugar esters using a stoichiometrical feed of substrates on a 10-g scale consisting of a reservoir open to atmosphere at 65 °C on a hot plate, a peristaltic pump and a packed-bed bioreactor (PBBR) filled with immobilized Rhizomucor Abstract The effects of high-speed homogenization, high-intensity ultrasound, and their combination were evaluated for the reduction of the particle size of sucrose crystals to enhance solvent-free lipase-catalyzed synthesis of sucrose oleate at 65 °C.…”

Regioselective Dehydration of Sugar Thioacetals under Mild Conditions

“…There have been recent reports on selective transformations of unprotected sugars and their derivatives using both biocatalytic 5,6,7,8 and chemical approaches 9,10,11 . De-oxygenation/dehydration of sugars is of particular interest and only a few approaches have been described.…”

Ionic Liquids: Efficient Media for the Lipase-Catalyzed Michael Addition

“…Very recently, the lipase-catalyzed esterification [ 20 ], ester-hydrolysis [ 21 ], and transesterification [ 22 ] reactions in the IL media have been widely reported. Furthermore, many reports have demonstrated that the ILs with the anions PF 6 – and BF 4 – were suitable reaction media for the lipase-catalyzed transesterification [ 22 , 23 ] and esterification [ 24 , 25 ] reactions. However, the anions PF 6 – and BF 4 – are hydrolytically unstable, which results in a propensity to decompose and release corrosive HF [ 26 , 27 ].…”