Enzymatic synthesis of 2-deoxyglucose-containing maltooligosaccharides for tracing the location of glucose absorption from starch digestion

Lee, Byung Hoo; Koh, Dong Wan; Territo, Paul R.; Park, Cheon Seok; Hamaker, Bruce R.; Yoo, Sangjun

doi:10.1016/j.carbpol.2015.06.012

Cited by 9 publications

(3 citation statements)

References 39 publications

(45 reference statements)

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“…The study revealed a close relationship between cumulative weight gain and energy bioavailability: high cumulative weights correlated with high bioavailable energy. Studies reveal that food nutrients are transformed in vivo following absorption to substrates that can produce metabolically useful energy (Lee et al., ; Sitrin, ). This transformation is done by intestinal enzymes, and the energy produced may either be stored or used to conduct biological processes such as growth and body maintenance (French, Epstein, Jeffery, Blundell, & Wardle, ).…”

Section: Discussionmentioning

confidence: 99%

Effect of sweet potato endogenous amylase activation on in vivo energy bioavailability and acceptability of soy‐enriched orange‐fleshed sweet potato complementary porridges

Birungi

Nabubuya

Mukisa

2018

Food Science & Nutrition

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Energy bioavailability can be influenced by food matrix factors and processing conditions or treatments. In this study, the effects of endogenous sweet potato amylase enzyme activation and slurry solids content of soy‐enriched orange‐fleshed sweet potato (OFSP) porridges on in vivo energy bioavailability (energy, weight gain, and feed efficiency ratio) and porridge acceptability were determined. Fifty‐six weanling albino rats were randomly assigned to two blocks each having eight groups of seven rats. The rats were housed in individual cages in a well‐ventilated animal house. The intervention block had rats fed on activated porridges (held at 75°C for 15 min), while rats in the control block were fed on nonactivated porridges (boiled at 90–95°C for 10 min). The rats were fed for 28 days on 50 ml of porridge per rat per day. The four groups per block were each fed on porridges with varying amounts of total solids content (10%, 15%, 20%, and 25%). Weight gain, energy bioavailability, and feed efficiency ratio were determined at the end of the feeding period. Consumer acceptability of activated and nonactivated porridges at 25% solids content was determined using a nontrained human panel (n = 40). Activation of amylases did not significantly (p > .05) affect the bioavailable energy, cumulative weight gain, and feed efficiency of the rats. Increasing slurry solids content of activated and nonactivated porridges significantly (p < .05) increased feed efficiency ratio (−14.6 ± 11.7 to 102.3 ± 2.3), weight gain (−1.4 to 5.6 g ± 1.9 g), and bioavailable energy (702.8 ± 16.2 to 1242.8 ± 12.2 kcal). Activation of amylases reduced porridge viscosity but did not significantly influence the overall acceptability. This work demonstrates the opportunity of utilizing sweet potato amylases to facilitate the preparation of complementary porridges with appropriate viscosity and increased energy density.

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Section: Discussionmentioning

confidence: 99%

Effect of sweet potato endogenous amylase activation on in vivo energy bioavailability and acceptability of soy‐enriched orange‐fleshed sweet potato complementary porridges

Birungi

Nabubuya

Mukisa

2018

Food Science & Nutrition

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“…The enzymatic reaction was terminated with the boiling water bath. 60 Enzymatic halohydrations of D-galactal, D-glucal, and L-fucal has been reported by Liu and his co-workers. 2halo-2-deoxy sugars were prepared by Chloroperoxidase-catalyzed Bromohydration and iodohydration reactions of three glycals in good yields (Scheme -19).…”

Section: Enzymatic Syntheses Of 2-dgmentioning

confidence: 94%

A review on synthetic methods for 2-deoxy-D-glucose

Singh¹,

Singh²,

Gupta³

2023

Arkivoc

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2-Deoxy-D-glucose (2-DG) is a non-metabolizable glucose analog that has shown promising pharmacological activities and has been used to study the role of glucose in cancer cells. 2-DG is an inhibitor of glycolysis, potential Energy Restriction Mimetic agent and inhibits pathogen-associated molecular patterns. Its radioisotope derivatives have application as tracers. Recently, 2-DG has been used as an anti-COVID-19 drug lowering the need for supplemental oxygen. In this review, different synthetic strategies for preparation of 2-DG including enzymatic synthesis have been discussed. The understanding of these methods would help in developing therapeutics or diagnostic agents aimed at exploring therapeutic targets related with energy metabolism.

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“…Currently, limited data exist as to the site of absorption of glucose produced from dietary starch breakdown within the small intestine. On‐going work has suggested promise in inserting bacterially synthesized 2‐deoxyglucose in starch molecules and tracking for this purpose . While SGLT1 and GLUT2 (which are expressed predominantly in the proximal regions of the small intestine, GLUT7 is expressed in limited amounts in the ileum.…”

Section: Absorption Of Glucose In the Gastrointestinal Tractmentioning

confidence: 99%

Starch digestion in the upper gastrointestinal tract of humans

Gill¹,

Wilcox

Pearson

et al. 2018

Starch Stärke

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Starch provides a large proportion of the dietary energy consumed worldwide. The breakdown of dietary starch is driven by α-amylase produced by the salivary glands and pancreatic acini and is completed by a range of brushborder bound enzymes. This enzymatic digestion is aided by mechanical and secretory actions of the gastrointestinal tract. The absorption of the resultant glucose in the small intestine is primarily driven by two separate transport proteins À SGLT1 and GLUT2. The control of processes that govern starch digestion is complex and still not fully understood, although it appears that the human gut has the ability to sense both glucose and non-sweet glucose oligomers. Recent work has also suggested that variations in the genes encoding for α-amylase also appear to be associated with health outcomes. The authors consider the physiological factors that govern starch digestion and absorption, consider other dietary factors that may impact on this process and attempt to highlight the limitations in current knowledge to help focus future research needs in relation to starch digestion the upper gastrointestinal tract.

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Enzymatic synthesis of 2-deoxyglucose-containing maltooligosaccharides for tracing the location of glucose absorption from starch digestion

Cited by 9 publications

References 39 publications

Effect of sweet potato endogenous amylase activation on in vivo energy bioavailability and acceptability of soy‐enriched orange‐fleshed sweet potato complementary porridges

Effect of sweet potato endogenous amylase activation on in vivo energy bioavailability and acceptability of soy‐enriched orange‐fleshed sweet potato complementary porridges

A review on synthetic methods for 2-deoxy-D-glucose

Starch digestion in the upper gastrointestinal tract of humans

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