Enzymatic synthesis and identification of oligosaccharides obtained by transgalactosylation of lactose in the presence of fructose using β-galactosidase from Kluyveromyces lactis

Shen, Qiuyun; Yang, Ruijin; Xiao, Hua; Ye, Fayin; Wang, He; Zhao, Wei; Wang, Kun

doi:10.1016/j.foodchem.2012.05.115

Cited by 42 publications

(12 citation statements)

References 27 publications

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“…Keto-disaccharides can be mainly synthesized by isomerization of the corresponding aldo-disaccharides using alkali, homogeneous or heterogeneous metal, and enzymatic catalysts. [7][8][9][10][11][12] Among the above methods, only alkali-catalyzed isomerization is versatile and can be reluctantly applied in food industry for reasons of food safety and productivity. However, even in alkalicatalyzed isomerization, a long time was required to attain reaction equilibrium and post-treatment processing was tedious.…”

mentioning

confidence: 99%

Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol

Gao

Kobayashi

Adachi

2016

Bioscience, Biotechnology, and Biochemistry

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Isomerization of disaccharides (maltose, isomaltose, cellobiose, lactose, melibiose, palatinose, sucrose, and trehalose) was investigated in subcritical aqueous ethanol. A marked increase in the isomerization of aldo-disaccharides to keto-disaccharides was noted and their hydrolytic reactions were suppressed with increasing ethanol concentration. Under any study condition, the maximum yield of keto-disaccharides produced from aldo-disaccharides linked by β-glycosidic bond was higher than that produced from aldo-disaccharides linked by α-glycosidic bond. Palatinose, a keto-disaccharide, mainly underwent decomposition rather than isomerization in subcritical water and subcritical aqueous ethanol. No isomerization was noted for the non-reducing disaccharides trehalose and sucrose. The rate constant of maltose to maltulose isomerization almost doubled by changing solvent from subcritical water to 80 wt% aqueous ethanol at 220 °C. Increased maltose monohydrate concentration in feed decreased the conversion of maltose and the maximum yield of maltulose, but increased the productivity of maltulose. The maximum productivity of maltulose was ca. 41 g/(h kg-solution).

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mentioning

confidence: 99%

Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol

Gao

Kobayashi

Adachi

2016

Bioscience, Biotechnology, and Biochemistry

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“…Other molecules possessing a hydroxyl group can be used as acceptor (e.g. sugars such as fructose [ 33 ], polyols such as glycerol [ 34 ], mannitol [ 35 ] and polyhydroxy acids such as gluconic acid [ 36 ]). In this case, the galactosyl moiety from lactose was transferred onto an ascorbic acid molecule as a result of activity of β-galactosidase produced by K. lactis .…”

Section: Resultsmentioning

confidence: 99%

Application of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside

Wojciechowska

Klewicki

Sójka

et al. 2017

Appl Biochem Biotechnol

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In view of a commonly known beneficial role and low stability of ascorbic acid, many efforts are constantly undertaken to produce its improved derivatives. This paper presents results on the synthesis of ascorbic acid galactoside using transgalactosylation properties of β-galactosidase from Kluyveromyces lactis and lactose as a donor of galactosyl moiety. The purpose of this study was to determine the influence of selected factors (concentration and molar ratio of substrates, amount of the enzyme preparation, pH of the solution, presence of different ions) on the course of transgalactosylation reaction. Research has shown that approx. 2.5% dry matter (d.m.; 12.7 g/L) of ascorbic acid galactoside is formed under favourable conditions (50% (w/v) substrates, sodium ascorbate and lactose at the molar ratio of 1.9:1, enzyme dose of 28,600 U/100 g lactose, pH = 7.0). The addition of Mg2+ or K+ ions to the reaction medium caused an increase in the final product content (even up to approx. 3.4% d.m., 17.2 g/L), while Na+ or Mn2+ had an adverse impact on the yield. The gathered data may be valuable for cosmetic or food industry.

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“…The recirculation cycle, or also known as the capacity of handling the feed stream, increases as the flow increases while the retention time reduces correspondingly as tabulated in Table 1. In the β-galactosidase-catalyzed reaction, lactose conversion is generally performed in two different pathways: (1) hydrolysis to glucose and galactose; and (2) transgalactosylation to GOS [34,35]. Hydrolysis occurs when water acts as galactosyl acceptor, yielding glucose and galactose as main products, [36,37].…”

Section: Biocatalytic Performance Of Psnf-β-galactosidasementioning

confidence: 99%

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

et al. 2020

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Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

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Enzymatic synthesis and identification of oligosaccharides obtained by transgalactosylation of lactose in the presence of fructose using β-galactosidase from Kluyveromyces lactis

Cited by 42 publications

References 27 publications

Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol

Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol

Application of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

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