Enzymatic Sacharification of Alkaline Pretreated Rice Straw by Cellulase From Cellulosimicrobium Sp. Mp1

Abstract

The effects of different physical and technological parameter such as time, substrate to liquid ratio, enzyme concentration, temperature, and pH on enzymatic saccharification of alkaline pretreated straw cellulose were studied. For alkaline pretreatment, the straw was incubated with 10 % NaOH at ratio 1:20 (w/v) at 90 °C for 1 hour. After the alkaline pretreatment the cellulose content increased from 50.2 % (w/w) to 67.3 % (w/w). Enzyme used for saccharification of treated and untreated straw was produced from Cellulosimicrobium sp MP1 which was isolated from termite gut. Results from research showed that the highest percentage of saccharification of alkaline pretreated straw was 69.91 %, corresponding to 10.58 mg/mL of reducing sugar. The hydrolysis conditions for reaching this highest saccharification yield were: temperature of 55 ºC, substrate to liquid ratio of 2 g/100 mL, enzyme concentration of 37.5 U/g, pH of 5.5 and hydrolysis time of 48 hours.