Enzymatic saccharification of pretreated wheat straw: Comparison of solids-recycling, sequential hydrolysis and batch hydrolysis

Pihlajaniemi, Ville; Sipponen, Satu; Sipponen, Mika H.; Pastinen, Ossi; Laakso, Simo

doi:10.1016/j.biortech.2013.11.060

Cited by 26 publications

(28 citation statements)

References 22 publications

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“…9 In view of emerging commercial scale bioreneries, it is important to understand how the AH process affects properties of both hemicellulose and lignin. These structural polymers are covalently linked in wheat straw, 10 forming the lignin-carbohydrate network that is susceptible to degrade during AH.…”

Section: Introductionmentioning

confidence: 99%

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Autohydrolysis and aqueous ammonia extraction of wheat straw: effect of treatment severity on yield and structure of hemicellulose and lignin

et al. 2014

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Section: Introductionmentioning

confidence: 99%

“…9 Total reducing sugars in the AH liquors were analyzed according to the DNS method using D-xylose standards. 16 The difference between the reducing sugar concentrations obtained from the AH liquors aer and before the dilute acid hydrolysis was interpreted as the amount of polymeric carbohydrate in the samples.…”

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Autohydrolysis and aqueous ammonia extraction of wheat straw: effect of treatment severity on yield and structure of hemicellulose and lignin

et al. 2014

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“…Nos dias atuais, a crescente preocupação com o desenvolvimento sustentável vem dando ênfase à busca por fontes alternativas de energia, fazendo com que a utilização de combustíveis fósseis seja gradualmente substituída por renováveis [1]. Dentro desse contexto, os resíduos agroindustriais lignocelulósicos estão em posição de destaque, uma vez que possuem potencial considerável para a obtenção de etanol a partir de processos fermentativos [2].…”

Section: Introductionunclassified

Pré-tratamento hidrotérmico de resíduos do milho visando à produção de etanol de segunda geração

Rocha¹,

Souza

Silva

et al. 2017

Sci. Plena

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Esse trabalho avaliou o potencial de produção de etanol de segunda geração (E2G) a partir da palha e do sabugo de milho. Os resíduos do milho foram submetidos ao pré-tratamento hidrotérmico (PTH), hidrólise enzimática e fermentação. O PTH foi realizado em reator de aço inoxidável em duas condições: 170°C/15 min e 195°C/10 min, em agitação de 200 rpm. As frações quantificadas foram: celulose, hemicelulose, lignina, cinzas, proteína e extrativos. Após pré-tratamento hidrotérmico, a amostra de palha apresentou 70,7% de remoção de hemicelulose a 170°C/15 min e 89,7% a 195°C/10 min. A menor perda de celulose ocorreu na condição mais branda de temperatura (170°C). Em relação ao sabugo, a remoção de hemicelulose foi de 58,7% a 170°C/15 min e 67,8% a 195°C/10 min. A menor perda de celulose foi obtida na maior temperatura (195°C). As amostras pré-tratadas foram submetidas a experimentos de hidrólise enzimática e fermentação. Obteve-se 87,5% de eficiência na fermentação para a palha pré-tratada a 170°C e 15 min e 86,9% para o sabugo pré-tratado a 195°C e 10 min. Os resultados indicam que os resíduos do milho são promissores para a obtenção de etanol de segunda geração visto que cerca de 2.500,000 mil litros de etanol podem ser obtidos a partir do processamento da palha e do sabugo de milho nas regiões centro-oeste, sul e sudeste do Brasil, sem aumentar as áreas de cultivo.Palavras-chave: biomassa lignocelulósica, pré-tratamento hidrotérmico, bioetanol.This work evaluated the potential of second generation ethanol production from husk and corn cob. Corn residues were submitted to hydrothermal pretreatment, enzymatic hydrolysis, and fermentation. The hydrothermal pretreatment was carried out in a stainless steel reactor under two operational conditions: 170°C/15 min and 195°C/10 min. Cellulose, hemicellulose, lignin, ash, protein, and extractives were the quantified components. After hydrothermal pretreatment, husk presented hemicellulose removal of 70.7% (170°C/15 min) and 89.7% (195°C/10 min). The cellulose lost was lower at 170°C. Hemicellulose removal in the corn assay was 58.7 % (170°C/15 min) and 67.8% (195°C/10 min). The cellulose lost was lower at 195°C. Enzymatic hydrolysis and fermentation experiments were conducted after pretreatment step. It was reached 87.5% of fermentative efficiency for the pretreated husk and 86.9% for the pretreated cob. The results suggest that corn residues are promising for second generation ethanol production since almost 2.500.000 ethanol liters can be obtained from processing husk and corn cob at midwest, south and southeast regions in Brazil, without increasing the harvest areas.

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“…However, optimal process conditions are not necessarily identical in processes with and without enzyme recycling (Lindedam et al, 2013). Ouyang et al (2013) employed the enzyme bound on residual substrate as a more effective method for recovery, although another experimental study pointed out that there is no actual accumulation of enzyme activity with solids recycling (Pihlajaniemi et al, 2014). Rodrigues et al (2014) found that ultrafiltration on a lab scale allowed the recovery of free enzyme with a recovery of 80% and highlighted the thermal stability of enzymes as essential for recycling.…”

Section: Introductionmentioning

confidence: 99%

Continuous and Semicontinuous Reaction Systems for High-Solids Enzymatic Hydrolysis of Lignocellulosics

Gonzalez‐Quiroga

Bula

Padilla

et al. 2015

Braz. J. Chem. Eng.

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-An attractive operation strategy for the enzymatic hydrolysis of lignocellulosics results from dividing the process into three stages with complementary goals: continuous enzyme adsorption at low-solids loading (5% w/w) with recycling of the liquid phase; continuous liquefaction at high-solids content (up to 20% w/w); and, finally, continuous or semicontinuous hydrolysis with supplementation of fresh enzymes. This paper presents a detailed modeling and simulation framework for the aforementioned operation strategies. The limiting micromixing situations of macrofluid and microfluid are used to predict conversions. The adsorption and liquefaction stages are modeled as a continuous stirred tank and a plug flow reactor, respectively. Two alternatives for the third stage are studied: a train of five cascading stirred tanks and a battery of batch reactors in parallel. Simulation results show that glucose concentrations greater than 100 g L -1 could be reached with both of the alternatives for the third stage.

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Enzymatic saccharification of pretreated wheat straw: Comparison of solids-recycling, sequential hydrolysis and batch hydrolysis

Cited by 26 publications

References 22 publications

Autohydrolysis and aqueous ammonia extraction of wheat straw: effect of treatment severity on yield and structure of hemicellulose and lignin

Autohydrolysis and aqueous ammonia extraction of wheat straw: effect of treatment severity on yield and structure of hemicellulose and lignin

Pré-tratamento hidrotérmico de resíduos do milho visando à produção de etanol de segunda geração

Continuous and Semicontinuous Reaction Systems for High-Solids Enzymatic Hydrolysis of Lignocellulosics

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