Enzymatic Saccharification of Laminaria japonica by Cellulase for the Production of Reducing Sugars

Abstract

Enzymatic saccharification of Laminaria japonica seaweed biomass was optimized by four independent factors (enzyme dose, hydrolysis time, pH, and temperature) using response surface methodology (RSM). To confirm the significance of the quadratic model, an analysis of variance (ANOVA) was performed, and the F-value of 8.76 showed that the regression model was highly significant (≤0.1%). In the accuracy study, average recoveries were in the range of 97.00% to 98.32%. The optimum experimental conditions were an enzyme dose of 8.2%, a hydrolysis time of 26 h, a pH of 4.1, and a temperature of 43°C. Temperature was the most important factor in the enzymatic saccharification. A relatively low temperature and short hydrolysis time were shown to improve the yield of reducing sugars.

Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

“…Utilizing information obtained from previous enzymatic hydrolysis [28], Celluclast ® 1.5 L (8.17% v/w biomass ), a hydrolysis time of 26.4 h, a pH of 4.1, and a temperature of 42.6 • C were selected as the predicted conditions. Enzymatic hydrolysis was conducted after the hydrothermal acid pretreatment under the predicted conditions using RSM.…”

“…Previous studies have reported combined treatment (sequential hydrolysis) to increase the reducing sugar yield [28]. Therefore, this study was aimed to perform sequential hydrolysis using hydrothermal acid pretreatment followed by enzymatic hydrolysis to determine the RS y .…”