Enzymatic removal of H‐2 alloantigens from the surface of P815‐(X2) mouse tumor cells

Thomas, Katherine; Engers, Howard; Cerottini, Jean‐Charles; Brunner, K. T.

doi:10.1002/eji.1830060405

Cited by 12 publications

(3 citation statements)

References 15 publications

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“…Complement-dependent antibody-mediated lysis of 'Cr-Iabeiled target cells was used to determine the alloaniibody litres of sera from alloimmune mice and was carried out as described previously [28].…”

Section: Methodsmentioning

confidence: 99%

Dissociation of the Humoral and Cell‐mediated Responses to Alloantigens in Mice by Sublethal Whole‐Body Irradiation

Engers

Louis

1979

Scand J Immunol

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Spleen cells from mice exposed to 500 rad sublethal whole-body irradiation before immunization with 100 x 10(6) allogeneic spleen cells exhibited an enhanced cytolytic T-lymphocyte (CTL) activity. In contrast, this treatment completely abrogated the humoral alloantibody response, as measured by complement-mediated lysis. In addition to the observed dissociation between primary humoral and cell-mediated responses, the character of the in vitro secondary CTL response of spleen cells obtained from mice sublethally irradiated before alloimmunization was also modified. Although these spleen cells were perfectly capable of mounting a secondary response upon restimulation in vitro with intact allogeneic spleen cells, they were no longer capable of responding to subcellular alloantigen preparations. These results suggest that the cellular requirements for the induction of secondary CTL responses in vitro depend upon the nature of the antigen used for restimulation.

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Section: Methodsmentioning

confidence: 99%

Dissociation of the Humoral and Cell‐mediated Responses to Alloantigens in Mice by Sublethal Whole‐Body Irradiation

Engers

Louis

1979

Scand J Immunol

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“…22), trypsinization of living cells does not completely remove H-2coded molecules from the cell surface. Thus, P815 tumour cells treated with trypsin could be lysed by either alloantibody and complement or CTL from C57BL/6 mice immunized with P815 [28]. In addition, H-2-coded antigens could be demonstrated by indirect immunofluorescence in trypsinized cells [7,28].…”

Section: B-cell Reactivity Against Sc-fitc Cannot Be Induced By Trypsmentioning

confidence: 99%

“…Thus, P815 tumour cells treated with trypsin could be lysed by either alloantibody and complement or CTL from C57BL/6 mice immunized with P815 [28]. In addition, H-2-coded antigens could be demonstrated by indirect immunofluorescence in trypsinized cells [7,28]. Since it was not tested whether cells treated with trypsin could induce CTL, it is not known whether those cells retained immunogenicity.…”

Section: B-cell Reactivity Against Sc-fitc Cannot Be Induced By Trypsmentioning

confidence: 99%

Cell Surface Structures Required for B‐Cell Activation with Haptenated Syngeneic Cells

Ramos

1983

Scand J Immunol

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The requirement for H-2-coded antigens on the cell surface of stimulator cells used for induction of B-cell responsiveness against haptenated (fluorescein isothiocyanate (FITC)) syngeneic cells was studied using H-2-less F9 teratocarcinoma cell lines. I found that FITC-labelled F9 cells, in contrast to normal spleen cells, could not induce hapten-specific antibody synthesis. The effect of treatment of stimulator cells with glutaraldehyde or trypsin before or after hapten labelling was also analysed. It was found that regardless of the order of treatment, hapten-specific antibody synthesis could not be induced by cells treated with glutaraldehyde or trypsin. In addition, hapten-specific B-cell unresponsiveness could not be induced by FITC-labelled glutaraldehyde-treated syngeneic lymphocytes. However, cold targets treated with trypsin or glutaraldehyde efficiently blocked T-cell-mediated cytotoxicity. The requirement for H-2-coded antigens for B-cell activation against haptenated syngeneic lymphocytes is discussed.

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Education of human lymphocytes against mouse cells: specific recognition of H‐2 antigens

et al. 1977

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INSERM U 25, HBpital Necker, ParisXeno-sensitization of human peripheral blood lymphocytes (PBL) against mouse lymphoid cells has been studied in vivo in a local graft-vs. -host (GVH) assay and in virro in a mixed lymphocyte culture-cell-mediated lympholysis system. Human PBL were injected into the footpads of mice rendered unresponsive by total-body irradiation, and these were subsequently tested for the presence of cytotoxic cells in the draining popliteal lymph node (LN). In spite of a definite PBL-induced LN proliferation, n o cytotoxic activity was detected against mouse target cells. In contrast with the GVH assay, human PBL collected after 7 days of in vitro culture in the presence of irradiated mouse cells, were strongly cytotoxic against mouse target cells. The antigenic specificities recogniied by in vitro educated cells were primarily those coded for by the H-2 complex. Only target cells with a n H-2 haplotype identical to that of the sensitizing mouse strain or with a t least an H-2D end in common with that strain were killed by xeno-sensitized lymphocytes. Mouse target cells derived from congenic resistant strains remained unaffected. It was verified that in vitro educated PBL depleted of B cells retained their cytotoxic effect, indicating that non-B cells and probably T cells were involved in the response .

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Enzymatic removal of H‐2 alloantigens from the surface of P815‐(X2) mouse tumor cells

Cited by 12 publications

References 15 publications

Dissociation of the Humoral and Cell‐mediated Responses to Alloantigens in Mice by Sublethal Whole‐Body Irradiation

Dissociation of the Humoral and Cell‐mediated Responses to Alloantigens in Mice by Sublethal Whole‐Body Irradiation

Cell Surface Structures Required for B‐Cell Activation with Haptenated Syngeneic Cells

Education of human lymphocytes against mouse cells: specific recognition of H‐2 antigens

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