Enzymatic reduction of hexavalent chromium by hexavalent chromium tolerant Pseudomonas ambigua G-1.

Horitsu, Hiroyuki; Futo, Satoshi; MIYAZAWA, Yoshimi; OGAI, Shusuke; Kawai, Keiichi

doi:10.1271/bbb1961.51.2417

Cited by 95 publications

(65 citation statements)

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“…In specific cases, hexavalent Cr serves as the terminal electron acceptor in the respiratory process of certain bacteria. This mechanism will indeed detoxify Cr (VI) while providing energy for bacterial cell growth [42].…”

Section: Resultsmentioning

confidence: 99%

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

2017

J App Biol Biotech

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Heavy metals can cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Bioremediation of polluted waters using these bacteria could be an alternative to conventional remediation methods. The aim of this study was to isolate and characterize heavy metal resistant probiotics. Soil samples were collected for the isolation of probiotics. Morphological, biochemical, and molecular characterization was performed for the isolates. In addition, a preliminary heavy metal minimum inhibitory concentration test was done followed by atomic absorption spectrometry (AAS) analysis analysis. Four Enterococci (BT1, BT2, MC1, and MC2) and Bacillus acidiproducens (SM1 and SM2) were isolated. Moreover, all the isolates demonstrated probiotic characteristics. BT1 and BT2 were able to tolerate mercury (Hg), cadmium (Cd), lead (Pb), and chromium (Cr) but they demonstrated poor Hg removal abilities (0.75-1.42%). MC1 and MC2 isolates could grow in medium supplemented with Cd, Pb, and Cr, respectively. MC1 showed the highest level of Pb removal (43.00% ± 0.776%) and Cd removal (46.19% ± 7.651%) from broth media. Yet, SM1 and SM2 isolates tolerated only Pb and Cr. SM2 had the ability to remove the highest amount of Cr (43.06% ± 7.991%). These reasonable heavy metal removal abilities could be further studied for efficient use in bioremediation.

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Section: Resultsmentioning

confidence: 99%

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

2017

J App Biol Biotech

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“…Cr(VI) reduction is shown to be cometabolic (not participating in energy conservation) in certain species of bacteria, but is predominantly dissimilatory/respiratory under anaerobic conditions (Ishibashi et al, 1990). In the latter process, Cr(VI) serves as a terminal electron acceptor in the membrane electron-transport respiratory pathway, a process resulting in energy conservation for growth and cell maintenance (Horitsu et al, 1987). Most micro-organisms are sensitive to Cr(VI), but some microbial species are resistant and can tolerate high levels of chromate.…”

Section: Biological Cr(vi) Reduction and Removalmentioning

confidence: 99%

“…In the latter process, Cr(VI) serves as a terminal electron acceptor in the membrane electron-transport respiratory pathway, a process resulting in energy conservation for growth and cell maintenance (Horitsu et al, 1987;Ishibashi et al, 1990). In the dissimilatory/respiratory process, electrons are donated from the electron donor to Cr(VI) via NADH (Chirwa & Wang, 1997a).…”

Section: Proposed Cr(vi) Reduction Mechanismsmentioning

confidence: 99%

“…In other studies, two pathways of Cr(VI) reduction are suggested for gram-negative bacteria (Figure 1). The first mechanism suggests Cr(VI) reduction mediated by a soluble reductase with NADH serving as the electron donor either by necessity (Horitsu et al, 1987) or for maximum activity (Ishibash et al, 1990). The NADH-dehydrogenase pathway is expected to predominate under aerobic conditions.…”

Section: Membrane Pathwaymentioning

confidence: 99%

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Biological Cr(VI) Reduction: Microbial Diversity, Kinetics and Biotechnological Solutions to Pollution

Chirwa¹,

Molokwane²

2011

Biodiversity

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“…Further evidence suggested that cytochrome c548 was involved in the reduction of Cr (VI) by membrane vesicles. In the presence of H2 and excess of hydrogenase, cytochrome C3, a periplasmic protein, in the soluble cell free fraction of D. vulgaris (Lovley and Coates, 1997), reduced Cr (VI), 50 times faster than did the Cr (VI) reductase of P. ambigua with NADH and NADPH, as electron donor (Horitsu et al, 1987). Under anaerobic condition Cr (VI) reduction is due to the action of enzymes associated with membranes of the electron transfer system (Cervantes and Campos, 2007).…”

Section: Introductionmentioning

confidence: 98%

Chromium (Vi) Reduction by Streptococcus Species Isolated from the Industrial Area of Abeokuta, Ogun State, Nigeria

Wani¹,

Zainab²,

Wasiu³

et al. 2015

Research J. of Microbiology

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Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study is to check the bacterial species for their tolerance towards multiple metals, antibiotics and plant growth promoting activity and further check whether these bacteria are reducing Cr (VI). Bacterial strains were isolated from metal contaminated soils of Abeokuta. All of the isolates showed tolerance to lead, zinc and chromium (VI). Bacterial specie also showed tolerance towards antibiotics, 100% of the isolates were tolerant to Septrin, Chloramphenicol, Sparfloxacin, Amoxicillin, Augmentin, Tarivid and Streptomycin, whereas 83.33% were tolerant to Gentamycin and Pefloxacin and 33.33% were resistant to Ciprofloxacin. All bacterial species were positive to ammonia, whereas strain PZ3 and PZ4 were found to be positive to HCN. Among all the strains, only Streptococcus spp. PZ4 showed reduction of Chromium (VI). Maximum reduction (85%) of chromium (VI) was observed at pH 7 by Streptococcus spp. PZ4. Similarly, Streptococcus spp. PZ4 reduced the chromium considerably at pH 5 (51.25%), pH 6 (72.5%), pH 8 (67.5%) and at pH 9 (45%), at a concentration of 100 µg Cr mLG after 120 h of incubation. Streptococcus spp. PZ4 1 reduced chromium (VI) at a concentration of 50 µg Cr mLG (47.5%), 100 µg Cr mLG (91.25%) and 1 1 150 µg Cr mLG (134.17%). Due to above properties strains could therefore be used a s 1 bioremediators of metals in soils contaminated with heavy metals and can also increase the yield of various crops under heavy metal contamination.

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Enzymatic reduction of hexavalent chromium by hexavalent chromium tolerant Pseudomonas ambigua G-1.

Cited by 95 publications

References 0 publications

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

Biological Cr(VI) Reduction: Microbial Diversity, Kinetics and Biotechnological Solutions to Pollution

Chromium (Vi) Reduction by Streptococcus Species Isolated from the Industrial Area of Abeokuta, Ogun State, Nigeria

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