Enzymatic Recovery of Elemental Palladium by Using Sulfate-Reducing Bacteria

Lloyd, Jon; Yong, Ping; Macaskie, Lynne E.

doi:10.1128/aem.64.11.4607-4609.1998

Cited by 272 publications

(135 citation statements)

References 19 publications

(21 reference statements)

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“…Bioreduction of Pd(II) by S. oneidensis cells resulted in recoveries higher than 90% of Pd associated with biomass when organic electron donors lactate or formate were used, regardless of the presence of O 2 or NO 3 -. Lloyd et al (1998) also found that sparging of cultures with air (O 2 ) only had a minor effect on Pd precipitation on D. desulphuricans. Interestingly, the slower bioreduction with H 2 gas resulted in relatively more small particles of bioPd on the cells, as can be observed by transmission electron microscopy (TEM).…”

Section: Discussionmentioning

confidence: 86%

“…The enzyme responsible for Pd(II) reduction has not been identified in studies with D. desulphuricans, but the involvement of a periplasmic hydrogenase was implicated by the consumption of hydrogen as electron donor and by the inhibition of the bioreduction with 0.5 mM Cu 2+ . A combination of hydrogenase with cytochrome c 3 activity has also been proposed (Lloyd et al, 1998). Since S. oneidensis has both [Ni/Fe] and [Fe] hydrogenases as well as cytochrome c 3 (Heidelberg et al, 2002), the proposed mechanism of Pd(II) reduction by D. desulphuricans could be valid for S. oneidensis.…”

Section: Discussionmentioning

confidence: 98%

“…Much like the process of gold precipitation by Pseudomonas aeruginosa described by Karthikeyan and Beveridge (2002), precipitation inside the periplasm was readily observed by TEM. It is likely that the participation of hydrogenase is required to initiate Pd(0) deposition on the cells (Lloyd et al, 1998;Baxter-Plant et al, 2003). The reduction of Pd(II) to Pd(0) later becomes self-sustaining via the ability of crystalline solid Pd(0) to absorb hydrogen and autocatalytically reduce more Pd(II) (Yong et al, 2002b).…”

Section: Discussionmentioning

confidence: 99%

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Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Windt

Aelterman

Verstraete

2005

Environmental Microbiology

332

196

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Microbial reduction of soluble Pd(II) by cells of Shewanella oneidensis MR-1 and of an autoaggregating mutant (COAG) resulted in precipitation of palladium Pd(0) nanoparticles on the cell wall and inside the periplasmic space (bioPd). As a result of biosorption and subsequent bioreduction of Pd(II) with H2, formate, lactate, pyruvate or ethanol as electron donors, recoveries higher than 90% of Pd associated with biomass could be obtained. The bioPd(0) nanoparticles thus obtained had the ability to reductively dehalogenate polychlorinated biphenyl (PCB) congeners in aqueous and sediment matrices. Bioreduction was observed in assays with concentrations up to 1000 mg Pd(II) l(-1) with depletion of soluble Pd(II) of 77.4% and higher. More than 90% decrease of PCB 21 (2,3,4-chloro biphenyl) coupled to formation of its dechlorination products PCB 5 (2,3-chloro biphenyl) and PCB 1 (2-chloro biphenyl) was obtained at a concentration of 1 mg l(-1) within 5 h at 28 degrees C. Bioreductive precipitation of bioPd by S. oneidensis cells mixed with sediment samples contaminated with a mixture of PCB congeners, resulted in dechlorination of both highly and lightly chlorinated PCB congeners adsorbed to the contaminated sediment matrix within 48 h at 28 degrees C. Fifty milligrams per litre of bioPd resulted in a catalytic activity that was comparable to 500 mg l(-1) commercial Pd(0) powder. The high reactivity of 50 mg l(-1) bioPd in the soil suspension was reflected in the reduction of the sum of seven most toxic PCBs to 27% of their initial concentration.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Windt

Aelterman

Verstraete

2005

Environmental Microbiology

332

196

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“…A promising alternative for removing Pd from solutions is the use of bacteria. The bioreductive deposition of small Pd(0) nanoparticles onto the cell surfaces of Desulfovibrio desulfuricans has been studied in detail (Lloyd et al 1998;Yong et al 2002a,b) and recovery of Pd from real processing waste was demonstrated (Yong et al 2002a). The properties of such nanoparticles usually differ significantly from those of the bulk material (Seifert 2004).…”

Section: Introductionmentioning

confidence: 99%

Manufacturing and characterization of Pd nanoparticles formed on immobilized bacterial cells

Pollmann

Merroun

Raff

et al. 2006

Lett Appl Microbiol

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Aims: To fabricate and analyse Pd nanoparticles on immobilized bacterial cells. Methods and Results: Biological ceramic composites (biocers) were used as a template to produce Pd(0) nanoparticles. The metal‐binding cells of the uranium mining waste pile isolate, Bacillus sphaericus JG‐A12 were used as a biological component of the biocers and immobilized by using sol‐gel technology. Vegetative cells and surface‐layer proteins of this strain are known to bind high amounts of Pd(II) that can be reduced to Pd(0) particles by the addition of a reducing agent. Sorption of Pd(II) by the biocers from a metal complex solution was studied by inductively coupled plasma mass spectroscopy analyses. After embedding into sol‐gel ceramics, the cells retained their Pd(II)‐binding capability. Pd(0) nanoclusters were produced by the addition of hydrogen as reducing agent after the sorption of Pd(II). The interactions of Pd(0) with the biocers and the formed Pd(0) nanoparticles were investigated by extended X‐ray absorption fine structure spectroscopy. The particles had a size of 0·6–0·8 nm. Conclusions: Bacterial cells that were immobilized by embedding into sol‐gel ceramics were used as a template to produce Pd nanoclusters of a size smaller than 1 nm. These particles possess interesting physical and chemical properties. Significance and Impact of the Study: The use of embedded bacterial cells as template enabled the fabrication of immobilized Pd(0) nanoparticles. These particles are highly interesting for technical applications, such as the development of novel catalysts.

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“…compounds. Besides SO 4 2− , SRB as a group are known to respire Fe(III), U(VI), Cr(VI), Tc(VII), Mo(VI), and Pd(II) (Lloyd, 2003;Lloyd, Yong, & Macaskie, 1998). Bio-reduction can lead to precipitation of metal oxides, phosphates, and carbonates (Lloyd, Mabbett, Williams, & Macaskie, 2001).…”

mentioning

confidence: 99%

pH‐dependent speciation and hydrogen (H₂) control U(VI) respiration by Desulfovibrio vulgaris

Kushwaha

Marcus

Rittmann

2018

Biotech & Bioengineering

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In situ bioreduction of soluble hexavalent uranium U(VI) to insoluble U(IV) (as UO ) has been proposed as a means of preventing U migration in the groundwater. This work focuses on the bioreduction of U(VI) and precipitation of U(IV). It uses anaerobic batch reactors with Desulfovibrio vulgaris, a well-known sulfate, iron, and U(VI) reducer, growing on lactate as the electron donor, in the absence of sulfate, and with a 30-mM bicarbonate buffering. In the absence of sulfate, D. vulgaris reduced >90% of the total soluble U(VI) (1 mM) to form U(IV) solids that were characterized by X-ray diffraction and confirmed to be nano-crystalline uraninite with crystallite size 2.8 ± 0.2 nm. pH values between 6 and 10 had minimal impact on bacterial growth and end-product distribution, supporting that the mono-nuclear, and poly-nuclear forms of U(VI) were equally bioavailable as electron acceptors. Electron balances support that H transiently accumulated, but was ultimately oxidized via U(VI) respiration. Thus, D. vulgaris utilized H as the electron carrier to drive respiration of U(VI). Rapid lactate utilization and biomass growth occurred only when U(VI) respiration began to draw down the sink of H and relieve thermodynamic inhibition of fermentation.

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Enzymatic Recovery of Elemental Palladium by Using Sulfate-Reducing Bacteria

Cited by 272 publications

References 19 publications

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Manufacturing and characterization of Pd nanoparticles formed on immobilized bacterial cells

pH‐dependent speciation and hydrogen (H₂) control U(VI) respiration by Desulfovibrio vulgaris

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Enzymatic Recovery of Elemental Palladium by Using Sulfate-Reducing Bacteria

Cited by 272 publications

References 19 publications

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls

Manufacturing and characterization of Pd nanoparticles formed on immobilized bacterial cells

pH‐dependent speciation and hydrogen (H2) control U(VI) respiration by Desulfovibrio vulgaris

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pH‐dependent speciation and hydrogen (H₂) control U(VI) respiration by Desulfovibrio vulgaris