Enzymatic reactions on immobilised substrates

Gray, Christopher; Weissenborn, Martin J.; Eyers, Patrick A.; Flitsch, Sabine L.

doi:10.1039/c3cs60018a

Cited by 79 publications

(51 citation statements)

References 246 publications

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“…Low thermal stability, narrow pH range, effective activity in water environment and the loss of catalytic activity after one cycle have been the greatest obstacles in the use of the enzymes in the multiple practical processes (Liese and Hilterhaus 2013;Gray et al 2013;DiCosimo et al 2013). However, the enormous catalytic potential offered by the enzymes for innumerable transformations, has stimulated intense studies aimed at the improvement of their properties (Mateo et al 2007;Brady and Jordon 2009;FernandezLafuente 2009;Garcia-Galan et al 2011;Cowan and Fernandez-Lafuente 2011;Rodrigues et al 2013).…”

Section: General Overviewmentioning

confidence: 99%

Enzyme immobilization by adsorption: a review

2014

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Endowed with unparalleled high catalytic activity and selectivity, enzymes offer enormous potential as catalysts in practical applications. These applications, however, are seriously hampered by enzymes' low thermal and chemical stabilities. One way to improve these stabilities is the enzyme immobilization. Among various tested methods of this process that make use of different enzyme-carrier interactions, immobilization by adsorption on solid carriers has appeared most common. According to these findings, in this review we present a comparative analysis of the literature reports on the recent trends in the immobilization of the enzymes by adsorption. This thorough study was prepared in order to provide a deeper understanding of the process. Both carriers, carrier modifiers and procedures developed for effective adsorption of the enzymes are discussed. The review may thus be helpful in choosing the right adsorption scheme for a given enzyme to achieve the improvement of its stability and activity for a specific application.

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Section: General Overviewmentioning

confidence: 99%

Enzyme immobilization by adsorption: a review

2014

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“…tyrosinases and peroxidases), are few systems employed for such immobilizations. [136–139] Recently, Sortase A (Srt A)-mediated ligation strategy has been established. [140,141] SrtA is a transpeptidase which recognizes a penta-peptide motif LPXTG (where X is any amino acid).…”

Section: Antibody Immobilization Chemistriesmentioning

confidence: 99%

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

Shen

Rusling

Dixit

2017

Methods

158

109

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Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.

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“…Gold nanoparticles can be utilized as surfaces for the study of enzymatic reactions, where their large surface area is a particular advantage. The ability of gold nanoparticles to present highly tailored surfaces, using self‐assembled monolayers (SAMs) of alkyl thiolates to display desired functionality, allows nanoparticles to mimic biomacromolecules and creates useful artificial platforms in water for the study biological systems .…”

Section: Figurementioning

confidence: 99%

A Bifunctional Spin Label for Ligand Recognition on Surfaces

et al. 2017

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In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin‐labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin‐labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy‐functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin‐labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose‐binding lectin, Con A, and the biotin‐binding protein avidin‐peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

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Enzymatic reactions on immobilised substrates

Cited by 79 publications

References 246 publications

Enzyme immobilization by adsorption: a review

Enzyme immobilization by adsorption: a review

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

A Bifunctional Spin Label for Ligand Recognition on Surfaces

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