Enzymatic production of xylooligosaccharides from alkali solubilized xylan of natural grass (Sehima nervosum)

Samanta, A.K.; Jayapal, Natasha; Kolte, Atul P.; Senani, S.; Sridhar, Manpal; Suresh, K; Sampath, K. T.

doi:10.1016/j.biortech.2012.02.036

Cited by 110 publications

(73 citation statements)

References 29 publications

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“…Mandelli et al (2014) evaluated the enzymatic production of XOs from sugarcane bagasse (cellulose 43.1%, hemicelluloses 25.2%, and lignin 22.9%) using a mixture of xylanase and feruloyl esterase. Samanta et al (2012) studied the production of XOs by xylanase hydrolysis from alkali solubilized xylan of natural grass (cellulose 37.25 ± 0.95%, hemicelluloses 28.10 ± 0.04%, and lignin 4.80 ± 0.30%). The results revealed that the highest xylobiose and xylotriose yields can be obtained at pH values from 5.03 to 5.11, temperatures from 40.33 to 45.19 °C, and reaction times from 10.11 to 16.55 h with xylanase doses from 13.20 to 17.41 U/g substrate.…”

Section: Enzymatic Hydrolysis Methodsmentioning

confidence: 99%

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

2016

BioResources

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Section: Enzymatic Hydrolysis Methodsmentioning

confidence: 99%

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

2016

BioResources

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“…The enzymatic hydrolysis of hard shell almond yielded 34.0% of XOs with 70% of purity [126]. A process for producing XOs from Sehima nervosum grass through enzymatic hydrolysis yielded 11 g/100 g xylan of xylobiose [127]. The treatment of wheat bran with the commercial xylanase preparation enzymes, produced a yield of approximately 31.2% of XOs, with a purity of 95% (w/w) and degree of polymerization of 2-7 [128].…”

Section: Xylanasesmentioning

confidence: 99%

Microbial Glycosidases for Nondigestible Oligosaccharides Production

Bezerra¹,

Montibra

Hansen

et al. 2017

Enzyme Inhibitors and Activators

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There is much interest in the study and production of nondigestible oligosaccharides (NDOs), due to their bioactivities and beneficial effects to the human health. The main approach in the production of NDOs relies on the action of glycosidases performing hydrolysis or transglycosylation of polysaccharides and sugars. In this chapter, a description of the main microbial glycosidases used for NDOs production, their sources, their principal properties, and a description of the production processes with the better results obtained are discussed.

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“…The xylan was weighed and ground in a grinder-mixer and stored at room temperature untill further analyses. The true and relative yield of xylan was calculated (Samanta et al 2012). The extraction condition that yielded highest xylan was followed for bulk extraction of xylan.…”

Section: Extraction Of Xylanmentioning

confidence: 99%

“…The enzyme hydrolysate from all the treatment combinations was collected and analyzed for reducing sugars (Somogyi 1952). The generation of XOS having different degree of polymerization was detected on thin layer chromatographic (TLC) plates (Samanta et al 2012). The compositional analysis of XOS mixture in the enzyme hydrolysate was carried out in HPLC; as described previously.…”

Section: Analysis Of Xylanmentioning

confidence: 99%

Value addition of corn husks through enzymatic production of xylooligosaccharides

Samanta¹,

Kolte²,

Elangovan³

et al. 2016

Braz. arch. biol. technol.

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Corn husks are the major wastes of corn industries with meagre economic significance. The present study was planned for value addition of corn husk through extraction of xylan, followed by its enzymatic hydrolysis into xylooligosaccharides, a pentose based prebiotic. Compositional analysis of corn husks revealed neutral detergent fibre 68.87%, acid detergent fibre 31.48%, hemicelluloses 37.39%, cellulose 29.07% and crude protein 2.68%. Irrespective of the extraction conditions, sodium hydroxide was found to be more effective in maximizing the yield of xylan from corn husks than potassium hydroxide (84% vs. 66%). Application of xylanase over the xylan of corn husks resulted into production of xylooligosaccharides with different degree of polymerization namely, xylobiose and xylotriose in addition to xylose monomer. On the basis of response surface model analysis, the maximum yield of xylobiose (1.9 mg/ml) was achieved with the enzymatic hydrolysis conditions of pH 5.8, temperature 44°C, enzyme dose 5.7U/ml and hydrolysis time of 17.5h. Therefore, the corn husks could be used as raw material for xylan extraction vis a vis its translation into prebiotic xylooligosaccharides.

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Enzymatic production of xylooligosaccharides from alkali solubilized xylan of natural grass (Sehima nervosum)

Cited by 110 publications

References 29 publications

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

Microbial Glycosidases for Nondigestible Oligosaccharides Production

Value addition of corn husks through enzymatic production of xylooligosaccharides

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