Enzymatic production of pyruvate from fumarate — an application of microbial cyclic-imide-transforming pathway

Ogawa, Jun; Soong, Chee-Leong; Ito, Masashi; Shimizu, Sakayu

doi:10.1016/s1381-1177(00)00024-2

Cited by 32 publications

(15 citation statements)

References 7 publications

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“…These process alternatives can be divided into three different approaches using: (i) enzymes (Burdick and Schaeffer, 1987;Eisenberg et al, 1997); (ii) resting cells (Izumi et al, 1982, Ogawa et al, 2001Schinschel and Simon, 1993); and (iii) fermentation processes (Li et al, 2001a(Li et al, , 2001b(Li et al, , 2002Yokota et al, 1994). In comparison to the other approaches, fermentation methods offer the opportunity to produce pyruvate from the sustainable, lowcost substrate, glucose, with high product/substrate yield, while avoiding the coproduction of unwanted byproducts like H 2 O 2 , as is usually the case when enzymatic methods are employed.…”

Section: Introductionmentioning

confidence: 99%

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Zelić

Gostović

Vuorilehto

et al. 2004

Biotech & Bioengineering

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Using the pyruvate production strain Escherichia coli YYC202 ldhA::Kan different process alternatives are studied with the aim of preventing potential product inhibition by appropriate product separation. This strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. Continuous experiments with cell retention, repetitive fed-batch, and an in situ product recovery (ISPR) process with fully integrated electrodialysis were tested. Although the continuous approach achieved a high volumetric productivity (QP) of 110 g L(-1) d(-1), this approach was not pursued because of long-term production strain instabilities. The highest pyruvate/glucose molar yield of up to 1.78 mol mol(-1) together with high QP 145 g L(-1) d(-1) and high pyruvate titers was achieved by the repetitive fed-batch approach. To separate pyruvate from fermentation broth a fully integrated continuous process was developed. In this process electrodialysis was used as a separation unit. Under optimum conditions a (calculated) final pyruvate titer of >900 mmol L(-1) (79 g L(-1)) was achieved.

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Section: Introductionmentioning

confidence: 99%

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Zelić

Gostović

Vuorilehto

et al. 2004

Biotech & Bioengineering

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“…In recent years, biocatalytic reactions and fermentation technology have started to replace conventional synthesis (Ogawa et al, 2001;Li et al, 2001a;Yokota et al, 1994;Gu et al, 2005). Several methods have been suggested to recover pyruvic acid from fermentation broth.…”

Section: Introductionmentioning

confidence: 99%

Study of a cleaner extraction of pyruvic acid from fermentation broth

Jin¹,

Liu²,

Xu³

et al. 2011

Afr. J. Biotechnol.

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“…Basically, there are four different known approaches to produce pyruvic acid: (1) chemical synthesis and oxidation (Tsujino et al, 1992;Ai and Ohdan, 1995), (2) enzymes (Burdick and Schaeffer, 1987;Eisenberg et al, 1997), (3) resting cells (Izumi et al, 1982;Schinschel and Simon, 1993;Ogawa et al, 2001), and (4) fermentation processes (Yokota et al, 1994;Li et al, 2001a;2001b;Causey et al, 2004). In comparison with these approaches, the fermentation processes is regarded as one of the most promising routes for the production of pyruvic acid.…”

Section: Introductionmentioning

confidence: 99%

Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glabrata TP19 in batch fermentation

Zhang

Nian-fa

2007

J. Zhejiang Univ. - Sci. B

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Abstract:Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 2 3 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R 2 ) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.

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Enzymatic production of pyruvate from fumarate — an application of microbial cyclic-imide-transforming pathway

Cited by 32 publications

References 7 publications

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Study of a cleaner extraction of pyruvic acid from fermentation broth

Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glabrata TP19 in batch fermentation

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