Enzymatic production of biohydrogen

A synthetic biochemistry molecular purge valve module that maintains redox balance

“…In vitro biotransformation systems have been reported in recent years involving systems of over 30 enzymes 13,14 . One of the first modern studies in this area was an artificial pathway that produced hydrogen from starch 1,[15][16][17] . The concept was recently advanced with a creative system that generated hydrogen from cellobiose at nearly 100% yields 16 .…”

Novel Hydrogen Bioreactor and Detection Apparatus

“…2a. Our apparatus mimics a similar system at Oak Ridge National Laboratory, which has been described previously in a number of biohydrogen experiments [13,14,16,[19][20][21], with Table 1. The custom bioreactor designed for this system is shown in Fig.…”

“…This concept was first pioneered by Woodward [13,14], who demonstrated the conversion of glucose and glucose 6-phosphate to hydrogen using first two, then eleven enzymes (respectively), and Zhang [15], who enabled the use of a lowercost substrate, starch. This later discovery, of enzymatic oxidation of a nonphosphorylated substrate completely to hydrogen and CO 2 , opened up the possibility of economical large-scale enzymatic hydrogen production.…”

Biofuel From Cellulosic Mass with Incentive for Feed Industry Employing Thermophilic Microbes

“…Hydrogen gas has been perceived one of future renewable energy resources as well as an environmentally compatible one as it does not evolves the "greenhouse gas" CO 2 at combustion (Abraham, 2002;Hansel & Lindblad, 1998;Matsunaga et al, 2000;Woodward et al, 1996).Further combustion of H 2 liberates large amounts of energy per unit weight and is easily converted to electricity by fuel cells (Miyamoto, 1993;Ueno et al, 1995;Rupprecht et al,2006). Hydrogen may be produced by electrolysis of water, thermocatalytic reformation of hydrogen-rich organic compounds and biological processes.…”