Enzymatic Production and Adipocyte Differentiation Inhibition of Low-Molecular-Weight-Alginate

Abstract

In this study, we investigated the extraction condition of alginate from Laminaria japonica, the enzymatic degradation of the extracted alginate, and the inhibitory activity of the degraded alginate on the differentiation of 3T3-L1 preadipocytes. The optimal conditions for the efficient extraction, precipitation, and recovery of alginate from the brown seaweed L. japonica were 1% for Na2CO3 concentration, 80℃ for extraction temperature, and ethanol for precipitation solvent. In the enzymatic reaction for the production of low-molecular-weight alginate (LMWA) by using alginate lyase from Flavobacterium sp., the initial concentration of Laminaria alginate was 3%. The low-molecular-weight degree from alginate was independent with the enzyme concentration, and the optimal concentration of alginate lyase was found to be 5 unit/ml. Through the enzymatic reaction with 5 unit/ml of alginate lyase at 37℃ for 3 hr, the viscosity and molecular weight of LMWA were 4.5 cp and 307 kDa, respectively. Treatment with LMWA significantly suppressed the accumulation of lipid droplet and triglyceride in 3T3-L1 preadipocytes with a dose-dependent manner. Therefore, it seems that LMWA treatment could inhibit the differentiation of 3T3-L1 preadipocytes. These results indicate that LMWA or the degraded alginate produced by alginate lyase enzyme can be useful for the development of anti-obesity biosubstances.

Introductory Chapter: Seaweed-Derived Biomaterials

“…Injectable alginate implants have been found to possess great potential in inhibiting the damaging events after myocardial infarction, leading to myocardial repair and tissue reconstruction [6]. Park et al [7] depolymerised alginate following extraction from Laminaria japonica, and they found that the alginate lyase enzyme-treated 307 kDa alginate reduced the accumulation of lipid droplet and triglyceride in 3 T3-L1 preadipocytes in a dose-dependent manner and can be developed further for antiobesity treatment. The TNF-α secretion-inducing activity of alginate oligomers following enzymatic (lyase) hydrolysis of polyguluronate and polymannuronate differed in RAW264.7 cells and was dependent on the oligomer structures [8].…”

Preparation of Stick Type Solid Glue as Paper Adhesive Using Mixed Seaweed Extract

“…Research Article 270 | Journal of Conservation Science Vol.35, No.4, 2019 These substances have been used in optical films for microorganisms, pharmacological carriers (Lai et al, 2003), and membranes (Perron et al, 2002). Alginic acid with low molecular is used to suppress adipocyte differentiation (Park et al, 2015) and senescence (Choi et al, 1992) in the biomedical field. It has been used also in the agricultural field as an application composite material for eco-friendly farm materials (Kang and Jung, 2016) and as an adsorbent and a material that can be used for qualitative and quantitative analyses (Choi and Choi, 2004;Kang et al, 2011).…”