Enzymatic processing of natural fibres: white biotechnology for sustainable development

Shahid, Mohammad Hasan; Mohammad, Faqeer; Chen, Guoqiang; Tang, Ren‐Cheng; Xing, Tieling

doi:10.1039/c6gc00201c

Cited by 103 publications

(44 citation statements)

References 232 publications

(301 reference statements)

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“…White biotechnology must contribute to the development of lignocellulose biorefineries by providing tailor-made microbial and enzymatic biocatalysts enabling “greener” and more efficient biotransformation routes for the complete use of both polysaccharides and lignin as the main biomass constituents [4, 5]. …”

Section: Introductionmentioning

confidence: 99%

Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Sáez-Jiménez

Rencoret

Rodrı́guez-Carvajal

et al. 2016

Biotechnol Biofuels

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BackgroundDespite claims as key enzymes in enzymatic delignification, very scarce information on the reaction rates between the ligninolytic versatile peroxidase (VP) and lignin peroxidase (LiP) and the lignin polymer is available, due to methodological difficulties related to lignin heterogeneity and low solubility.ResultsTwo water-soluble sulfonated lignins (from Picea abies and Eucalyptus grandis) were chemically characterized and used to estimate single electron-transfer rates to the H2O2-activated Pleurotus eryngii VP (native enzyme and mutated variant) transient states (compounds I and II bearing two- and one-electron deficiencies, respectively). When the rate-limiting reduction of compound II was quantified by stopped-flow rapid spectrophotometry, from fourfold (softwood lignin) to over 100-fold (hardwood lignin) lower electron-transfer efficiencies (k 3app values) were observed for the W164S variant at surface Trp164, compared with the native VP. These lignosulfonates have ~20–30 % phenolic units, which could be responsible for the observed residual activity. Therefore, methylated (and acetylated) samples were used in new stopped-flow experiments, where negligible electron transfer to the W164S compound II was found. This revealed that the residual reduction of W164S compound II by native lignin was due to its phenolic moiety. Since both native lignins have a relatively similar phenolic moiety, the higher W164S activity on the softwood lignin could be due to easier access of its mono-methoxylated units for direct oxidation at the heme channel in the absence of the catalytic tryptophan. Moreover, the lower electron transfer rates from the derivatized lignosulfonates to native VP suggest that peroxidase attack starts at the phenolic lignin moiety. In agreement with the transient-state kinetic data, very low structural modification of lignin, as revealed by size-exclusion chromatography and two-dimensional nuclear magnetic resonance, was obtained during steady-state treatment (up to 24 h) of native lignosulfonates with the W164S variant compared with native VP and, more importantly, this activity disappeared when nonphenolic lignosulfonates were used.ConclusionsWe demonstrate for the first time that the surface tryptophan conserved in most LiPs and VPs (Trp164 of P. eryngii VPL) is strictly required for oxidation of the nonphenolic moiety, which represents the major and more recalcitrant part of the lignin polymer.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0615-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Sáez-Jiménez

Rencoret

Rodrı́guez-Carvajal

et al. 2016

Biotechnol Biofuels

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“…The combination of the dyeing properties with antioxidative and antimicrobial activity of phenazines is very desirable in the textile industry. It prevents both fading of textiles and colonization of bacteria; therefore, it can be used for medical purposes, especially in hospitals with strict sanitary requirements as well as by immunocompromised patients [31].…”

Section: Discussionmentioning

confidence: 99%

Structure and Bioactive Properties of Novel Textile Dyes Synthesised by Fungal Laccase

Polak

Wlizło

Pogni

et al. 2020

IJMS

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Novel sustainable processes involving oxidative enzymatic catalysts are considered as an alternative for classical organic chemistry. The unique physicochemical and bioactive properties of novel bio-products can be obtained using fungal laccase as catalyst. Among them are textile biodyes synthesised during oxidation of substrates belonging to the amine and methoxy organic derivatives. The process of synthesis occurs in mild conditions of pH, temperature, and pressure, and without using harmful oxidants. The effect of fungal laccase activity on the substrates mixture transformation efficiency was analysed in terms of antimicrobial dye synthesis on a large scale. Three new phenazine dyes, obtained in the presence of laccase from Cerrena unicolor, were studied for their structure and properties. The phenazine core structure of the products was a result of tri-molecular transformation of aminomethoxybenzoic acid and aminonaphthalene sulfonic acid isomers. One of the compounds from the synthesised dye, namely 10-((2-carboxy-6-methoxyphenyl)amino)-11-methoxybenzo[a]phenazine-8-carboxylic acid, was able to inhibit the growth of Staphylococcus aureus. The high concentration of substrates (5 g/L) was efficiently transformed during 72 h in the mild conditions of pH 4 with the use of laccase with an activity of 200 U per g of the substrates mixture. The new bioactive dye exhibited excellent dyeing properties with concomitant antibacterial and antioxidative activity. The proposed enzyme-mediated synthesis represents an alternative eco-friendly route for the synthesis of novel antimicrobial compounds with high importance for the medical textile industry.

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“…Biotechnology has various industrial applications including the manufacture of chemicals and biopharmaceuticals, bio-polymers and bio plastics, food, feed and biofuels [16]. White biotechnology or industrial biotechnology uses enzymes and micro-organisms to make bio-based products, including chemicals, food and feed, bioenergy, and textiles [10,[18][19][20][21][22]. Gray biotechnology is comprised from technological solutions created to protect the environment, like in the case of oil spills and purifying sewage water [23].…”

Section: Definition Of Bioeconomymentioning

confidence: 99%

Bioeconomic Assessment of Microalgal Production

Özçimen¹,

İnan²,

Koçer³

et al. 2018

Microalgal Biotechnology

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Today, microalgae play an important role for the worldwide biofuel demand, together with the production of high value-added products used in pharmaceutical, nutraceutical and cosmetic industries. In 2014, the European Union adopted a strategy for developing the bioeconomy, by utilizing microalgae which represent an emerging biological resource of great importance for its potential applications in different fields. Huge potential of tiny microalgae could support a microalgae-based biorefinery and microalgae-based bioeconomy opening up vast opportunities in the global algae business. Nevertheless, in spite of having been studied for over 50 years now, there are still only just a few corporations that are cultivating algae on a large or commercial scale due to operational and capital cost. Techno-economic modeling is a powerful tool for guiding research priorities and assessing the economics, environmental impact and sustainability of microalgal productions. In this chapter, microalgal productions are assessed within bioeconomical aspects and case-studies on microalgal biorefinery are discussed.

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Enzymatic processing of natural fibres: white biotechnology for sustainable development

Abstract: Industrial biotechnology has the potential to fulfil many key criteria of greener textile production.

Cited by 103 publications

References 232 publications

Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Structure and Bioactive Properties of Novel Textile Dyes Synthesised by Fungal Laccase

Bioeconomic Assessment of Microalgal Production

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