Enzymatic Polymerization

Kobayashi, Shiro; Uyama, Hiroshi; Kimura, Shunsaku

doi:10.1021/cr990121l

Cited by 863 publications

(603 citation statements)

References 354 publications

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“…However, the premixing of the reactive streams before the formation of droplets often resulted in the blockage of small opening of the microfluidics. Enzymatic reaction has been extensively studied owing to its low toxicity, mild reaction, stereochemistry, and high reaction velocity, high enantio-, regio-and chemo-selectivity [24]. Horseradish peroxidise/hydrogen peroxide (HRP/H 2 O 2 ) is a common enzymatic system, where peroxidases (oxidoreductases) catalyzes the oxidation of donors using H 2 O 2 , resulting in polyphenols linked at the aromatic ring by C-C and C-O coupling of phenols [25].…”

Section: Introductionmentioning

confidence: 99%

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

Ke¹,

Liu²,

Wang

et al. 2014

Express Polym. Lett.

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Abstract. Biocompatible and biodegradable carboxymethyl cellulose (CMC) has been modified with 4-hydroxybenzylamine (CMC-Ph) in order to prepare CMC-based microgels through the horseradish peroxidise/hydrogen peroxide enzymatic reaction. CMC-Ph was identified as a blend, and the amount of the grafted 4-hydroxybenzylamine per 100 units of CMC was between 17 and 23 according to the molecular weight of CMC. Through a special designed co-flowing microfluidic device, CMC-Ph microgels were prepared with the radius from 100 to 500 µm via adjusting the flow rates of the disperse phase and the continuous phase, respectively. The chondrocytic cell line ATDC5 was encapsulated in the CMC-Ph microgels. The cell-laden microgels were cultured for up to 40 days, illustrating the biocompatibility of CMC-Ph and the microfluidic approach through the enzymatic crosslinking reaction primarily. CMC-Ph showed a great promise to encapsulate the cells for further fabrication of the injectable scaffolds.

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Section: Introductionmentioning

confidence: 99%

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

Ke¹,

Liu²,

Wang

et al. 2014

Express Polym. Lett.

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“…These difficulties pose a serious barrier for non-specialists of organic chemistry to participate in cellulose chemistry. The second approach to access cellulose derivatives is enzymatic polymerization of chemically modified glucoside monomers to the corresponding cellulose derivatives (bottom-up approach) [19][20][21]. The most potential advantage of this approach is perfect structural homogeneity of the resultant cellulose derivatives.…”

Section: Introductionmentioning

confidence: 99%

Cellulose Chemistry Meets Click Chemistry: Syntheses and Properties of Cellulose-Based Glycoclusters with High Structural Homogeneity

et al. 2011

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-1,4-Glucans having oligosaccharide appendages (O-/N-linked -maltoside and O-/N-linked -lactoside) at 6C positions of all repeating units can be readily prepared from cellulose through a two step strategy composed of: (1) regio-selective and quantitative bromination/azidation to afford 6-azido-6-deoxycellulose; and (2) the subsequent Cu + -catalyzed coupling with oligosaccharides having terminal alkyne. The resultant cellulose derivatives showed improved water solubility in comparison to native cellulose; they, however, bound to carbohydrate-binding proteins in a rather non-specific manner. Molecular dynamics calculations revealed that these properties are attributable to rigid sheet-like structures of the cellulose derivatives and the subsequent exposure of their hydrophobic moieties to solvents.

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“…[1][2][3] Various biopolymers and non-natural synthetic polymers have been synthesized under ecofriendly and milder conditions by enzyme-catalyzed polymerizations. A selective enzyme catalyzes a specific polymerization reaction with high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic polymerization of acrylamide with hydrogen peroxide catalyzed by water-soluble anionic iron(III) 5,10,15,20-tetrakis-(2',6'-dichloro-3'-sulfonatophenyl)porphyrin

Angrish¹,

Chauhan²

2004

Arkivoc

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The biomimetic polymerization of acrylamide (1) with hydrogen peroxide catalyzed by water soluble iron(III) 5,10,15,20-tetrakis-(2′,6′-dichloro-3′-sulfonatophenyl)porphyrin (3) gave high yield of polyacrylamide (2) in the molecular weight range of 1,30,000-1,50,000 g mol -1 in the absence and presence of 2,4-pentanedione at an ambient temperature in nitrogen atmosphere. The molar ratio of 3: H 2 O 2 : 1 was 1: 100: 3300. Yields were high at pH 4.5 but dropped at pH 7.2. Finally no polymer was formed at pH 9.2 in the absence of 2,4-pentanedione, whereas the yield raised to 99% in the presence of 2,4-pentanedione.

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Enzymatic Polymerization

Cited by 863 publications

References 354 publications

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

Preparation of carboxymethyl cellulose based microgels for cell encapsulation

Cellulose Chemistry Meets Click Chemistry: Syntheses and Properties of Cellulose-Based Glycoclusters with High Structural Homogeneity

Biomimetic polymerization of acrylamide with hydrogen peroxide catalyzed by water-soluble anionic iron(III) 5,10,15,20-tetrakis-(2',6'-dichloro-3'-sulfonatophenyl)porphyrin

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