Enzymatic Oxidative Transformation of Chlorophenol Mixtures

Bollag, Jean Marc; Chu, Horng-Lun; Rao, María A.; Gianfreda, Liliana

doi:10.2134/jeq2003.0063

Cited by 27 publications

(5 citation statements)

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“…The typical catalytic action of laccase involves oxidation of the phenolic substrates by H‐abstraction transferring the electrons to dioxygen (one‐electron oxidation). This results in the formation phenoxy radicals that in turn may undergo disproportionation to quinones or coupling to humic substance‐like polymerization products . However, in our study, p ‐benzoquinone was not observed in HPLC elution profiles of phenol samples treated with laccase; only dark colored products that partly precipitated were found.…”

Section: Resultscontrasting

confidence: 79%

Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge

Carabajal

Perullini

Jobbágy

et al. 2015

CLEAN Soil Air Water

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Research Article Removal of Phenol by Immobilization of Trametes versicolor in Silica-Alginate-Fungus Biocomposites and Loofa SpongeWhite-rot fungi have potential in organic pollutant degradation. Immobilization of microorganisms has been successfully used for bioremediation. In this work, 25 isolates of Argentinean white-rot fungi were tested for their tolerance toward up to 10 mM phenol in agar plates. Seven isolates were further evaluated for their ability to grow on plates with 2,6-dimethoxyphenol, gallic acid, 2,4-dichlorophenol, or guaiacol (7.5 mM), or with phenol as sole carbon source. Best results were obtained with Trametes versicolor, Irpex lacteus, Lentinus tigrinus, and Pleurotus lindquistii. The ability of immobilized cultures of T. versicolor BAFC 2234 to remove phenol, was studied. Silica-alginate-fungus biocomposites resulted in phenol removal (10 mM) of up to 48% (mainly attributable to biosorption) within 14 days. Immobilized on Luffa aegyptica, it removed between 62 and 74% of phenol (15 mM) in three repeated cycles over a period of 23 days. Laccase was the main oxidative enzyme detected, and the purified enzyme oxidized 84% of phenol (0.5 mM) in vitro within 4 h, while 43% was converted by a purified Mn-peroxidase. The phenol phytotoxicity decreased noticeably. The concentrations of phenol removed are among the highest reported so far, thus this strain of T. versicolor may have good prospects for application in industrial wastewater treatment.

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Section: Resultscontrasting

confidence: 79%

Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge

Carabajal

Perullini

Jobbágy

et al. 2015

CLEAN Soil Air Water

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“…Laccases offer several advantages of great interest for biotechnological applications. They exhibit broad substrate specificity and are thus able to oxidize a broad range of xenobiotic compounds including chlorinated phenolics (Royarcand & Archibald, 1991; Roper et al , 1995; Ullah et al , 2000; Schultz et al , 2001; Bollag et al , 2003), synthetic dyes (Chivukula & Renganathan, 1995; Rodriguez et al , 1999; Wong & Yu, 1999; Abadulla et al , 2000; Nagai et al , 2002; Claus et al , 2002; Soares et al , 2002; Peralta‐Zamora et al , 2003; Wesenberg et al , 2003; Zille et al , 2003), pesticides (Nannipieri & Bollag, 1991; Jolivalt et al , 2000; Torres et al , 2003) and polycyclic aromatic hydrocarbons (Johannes et al , 1996; Collins et al , 1996; Majcherczyk et al , 1998; Majcherczyk & Johannes, 2000; Cho et al , 2002; Pozdnyakova et al , 2004). They can bleach Kraft pulp (Reid & Paice, 1994; Paice et al , 1995; Bourbonnais & Paice, 1996; Call & Mucke, 1997; Monteiro & de Carvalho, 1998; de Carvalho et al , 1999; Sealey et al , 1999; Balakshin et al , 2001; Lund et al , 2003; Sigoillot et al , 2004) or detoxify agricultural byproducts including olive mill wastes or coffee pulp (D'Annibale et al , 2000; Tsioulpas et al , 2002; Velazquez‐Cedeno et al , 2002) (for review see Durán & Esposito, 2000; Durán et al , 2002; Mayer & Staples, 2002).…”

Section: Laccases In Environmental Biotechnologymentioning

confidence: 99%

Fungal laccases – occurrence and properties

2006

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Laccases of fungi attract considerable attention due to their possible involvement in the transformation of a wide variety of phenolic compounds including the polymeric lignin and humic substances. So far, more than a 100 enzymes have been purified from fungal cultures and characterized in terms of their biochemical and catalytic properties. Most ligninolytic fungal species produce constitutively at least one laccase isoenzyme and laccases are also dominant among ligninolytic enzymes in the soil environment. The fact that they only require molecular oxygen for catalysis makes them suitable for biotechnological applications for the transformation or immobilization of xenobiotic compounds.

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“…The contaminant considered was 2,4,5-trichlorophenol, a compound that is commonly used in both the timber industry -to protect recently cut wood from fungal attack before it is removed from the forest-and in agriculture as it is a precursor in the synthesis of various herbicides (Annachhatre and Gheewala, 1996;Bollag et al, 2003). As 2,4,5-trichlorophenol is sparingly soluble in water (Czaplika, 2004), it was first mixed with quartz sand (in the proportions required to generate the doses indicated below) before being added to the soil, and this mixture was then shaken for 48 hours in a rotary shaker to achieve homogeneity (Moscoso et al, 2007).…”

Section: Contamination Experimentsmentioning

confidence: 99%

Evaluation of various tests for the diagnosis of soil contamination by 2,4,5-trichlorophenol (2,4,5-TCP)

Bello

Trasar-Cepeda

Leirós

et al. 2008

Environmental Pollution

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The concentration of a toxic substance present in a soil cannot be considered as the only criteria to classify such soil as contaminated. AbstractThe response of different types of soils to contamination with 2,4,5-triclorophenol was studied to test the validity of the concept of generic reference levels (GRL), the main criterion used to define soil contamination. Soil samples were artificially contaminated with doses of between 0 and 5000 mg kg -1 of 2,4,5-triclorophenol, and analysed by various tests. Where possible, the response of soils to the contaminant was modelled by a sigmoidal dose-response curve in order to estimate the ED 50 values. The tests provided different responses, but only microbial biomass-C and dehydrogenase and urease activities demonstrated soil deterioration in response to contamination. The results suggest that the diagnosis of soil contamination has been greatly simplified in the legislation by the provision of a single figure for each compound, and that the GRL concept could perhaps be substituted by measurement of ED 50 values, which better reflect the alteration of a soil due to the presence of a xenobiotic substance.

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Enzymatic Oxidative Transformation of Chlorophenol Mixtures

Cited by 27 publications

References 0 publications

Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge

Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge

Fungal laccases – occurrence and properties

Evaluation of various tests for the diagnosis of soil contamination by 2,4,5-trichlorophenol (2,4,5-TCP)

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