Enzymatic iron and uranium reduction by sulfate-reducing bacteria

Lovley, Derek R.; Roden, Eric E.; Phillips, Elizabeth; Woodward, Joan C.

doi:10.1016/0025-3227(93)90148-o

Cited by 483 publications

(334 citation statements)

References 45 publications

Supporting

Mentioning

309

Contrasting

Unclassified

Order By: Relevance

“…Owing to this ability to reduce and accumulate heavy metals and radionuclides (Jones et al, 1976;Lovley et al, 1993a;Chardin et al, 2002), sulfate-reducing bacteria (SRBs) have drawn particular attention for potential applications in heavy metal immobilization. It has been well documented that SRBs can reductively precipitate redox metals through enzymatic pathways (Lovley and Phillips, 1992;Abdelouas et al, 1998) or can simply precipitate metals as metallic sulfides.…”

Section: Introductionmentioning

confidence: 99%

Impact of elevated nitrate on sulfate-reducing bacteria: a comparative Study of Desulfovibrio vulgaris

Joyner

et al. 2010

The ISME Journal

View full text Add to dashboard Cite

Sulfate-reducing bacteria have been extensively studied for their potential in heavy-metal bioremediation. However, the occurrence of elevated nitrate in contaminated environments has been shown to inhibit sulfate reduction activity. Although the inhibition has been suggested to result from the competition with nitrate-reducing bacteria, the possibility of direct inhibition of sulfate reducers by elevated nitrate needs to be explored. Using Desulfovibrio vulgaris as a model sulfate-reducing bacterium, functional genomics analysis reveals that osmotic stress contributed to growth inhibition by nitrate as shown by the upregulation of the glycine/betaine transporter genes and the relief of nitrate inhibition by osmoprotectants. The observation that significant growth inhibition was effected by 70 mM NaNO 3 but not by 70 mM NaCl suggests the presence of inhibitory mechanisms in addition to osmotic stress. The differential expression of genes characteristic of nitrite stress responses, such as the hybrid cluster protein gene, under nitrate stress condition further indicates that nitrate stress response by D. vulgaris was linked to components of both osmotic and nitrite stress responses. The involvement of the oxidative stress response pathway, however, might be the result of a more general stress response. Given the low similarities between the response profiles to nitrate and other stresses, less-defined stress response pathways could also be important in nitrate stress, which might involve the shift in energy metabolism. The involvement of nitrite stress response upon exposure to nitrate may provide detoxification mechanisms for nitrite, which is inhibitory to sulfate-reducing bacteria, produced by microbial nitrate reduction as a metabolic intermediate and may enhance the survival of sulfate-reducing bacteria in environments with elevated nitrate level.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of elevated nitrate on sulfate-reducing bacteria: a comparative Study of Desulfovibrio vulgaris

Joyner

et al. 2010

The ISME Journal

View full text Add to dashboard Cite

show abstract

“…Based on findings that a number of Fe-and S-reducing bacteria also reduce U(VI), enzymatically as well as indirectly [7][8][9][10] , a strategy under development for remediating U-contaminated soil and groundwater is based on promoting reductive precipitation of low solubility U(IV) minerals. Emerging techniques rely on injecting organic carbon (OC) into contaminated sediments in order to stimulate direct or indirect microbial U(VI) reduction to U(IV) solids 2-4 .…”

mentioning

confidence: 99%

Reoxidation of Bioreduced Uranium under Reducing Conditions

Wan

Tokunaga

Brodie

et al. 2005

Environ. Sci. Technol.

164

185

View full text Add to dashboard Cite

“…The group of uranium-reducing microorganisms now includes several sulfate-reducing bacteria, and mechanistic investigation of Desulfovibrio vulgaris has shown that the respiratory electron transport protein cytochrome c, catalyzes the U(VI) reduction (Lovley et al 1993b). Sulfate-reducing bacteria do not appear to obtain energy for growth from the process, however (Lovley et al 1993a).…”

Section: Dissimilatory Metal Reductionmentioning

confidence: 99%