Enzymaticin situsaccharification of rice straw in aqueous-ionic liquid media using encapsulatedTrichoderma aureoviridecellulase

Abstract

BACKGROUND: Enzymatic in situ saccharification of lignocellulose in ionic liquids (ILs) has become a hot topic of research, but is hampered by the incompatibility of ILs with cellulase. The aim of this present work was to improve IL tolerance of the cellulase from Trichoderma aureoviride strain HS through an efficient encapsulation method, and thus to develop a compatible IL-cellulase system for biorefining.

Measurement of high pressure densities and atmospheric pressure viscosities of alkyl phosphate anion ionic liquids and correlation with the ε ∗ -modified Sanchez-Lacombe equation of state

“…Hydrogen bond basicity (β) of the ionic liquid is considered to be the dominant physical property for biomass dissolution [1] with the minimum value required for β being equal to 0.8 [2]. Application of alkyl phosphate anion ionic liquids with the objective of degrading or pretreating biomass have been studied for corn [10,11], rice straw [11,12], sugarcane bagasse [13][14][15], barley starch [16] and cellulose [3,17,18]. Application of alkyl phosphate anion ionic liquids with the objective of degrading or pretreating biomass have been studied for corn [10,11], rice straw [11,12], sugarcane bagasse [13][14][15], barley starch [16] and cellulose [3,17,18].…”

Biocatalysis and Biomass Conversion in Alternative Reaction Media

“…[136] Hybrid cellulase CLEAs containing as ilica core, prepared by physical adsorption of cellulose CLEAs on ah ighly porous silica support, exhibited twice as much activity as the regular CLEA, and settled better after the hydrolysis, thus facilitating its separation. [140] Ogino and co-workersw ent as tep furtheri ni ntegrationo fl ignocellulose to bioethanol. They have the added advantage that they can be magnetically separated from suspensions of other solids, as is the case with lignocellulosic biomass.…”

“…However, to our knowledge, such magnetic cellulase CLEAs have not yet been used in IL‐ or DES‐containing media. Xu and co‐workers used a cellulase from Trichoderma aureoviride , encapsulated in alginate beads, in the enzymatic in situ saccharification of rice straw pretreated in aqueous [emim][(MeO) 2 PO 2 ] . Ogino and co‐workers went a step further in integration of lignocellulose to bioethanol .…”

ChemInform Abstract: Biocatalysis and Biomass Conversion in Alternative Reaction Media

“…Xu and co-workers used ac ellulase from Trichoderma aureoviride,e ncapsulated in alginateb eads, in the enzymatic in situ saccharification of rice straw pretreated in aqueous [emim][(MeO) 2 PO 2 ]. [140] Ogino and co-workersw ent as tep furtheri ni ntegrationo fl ignocellulose to bioethanol. [141] They combined pretreatmenta nd saccharification with fermentation by ac ellulase-displaying, ionic liquidresistantyeast into ao ne-pot process.…”