Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design

Abstract

The enzymatic conversion of lignocellulosic material to sugars can provide a carbon source for the production of energy (fuels) and a wide range of renewable products. However, the efficiency of this conversion is impaired due to product (sugar) inhibition. Even though several studies investigate how to overcome this challenge, concepts on the process to conduct the hydrolysis are still scarce in literature. Aqueous two-phase systems (ATPS) can be applied to design an extractive reaction due to their capacity to partition solutes to different phases in such a system. This work presents strategies on how to conduct extractive enzymatic hydrolysis in ATPS and how to explore the experimental results in order to design a feasible process. While only a limited number of ATPS was explored, the methods and strategies described could easily be applied to any further ATPS to be explored. We studied two promising ATPS as a subset of a previously high throughput screened large set of ATPS, providing two configurations of processes having the reaction in either the top phase or in the bottom phase. Enzymatic hydrolysis in these ATPS was performed to evaluate the partitioning of the substrate and the influence of solute partitioning on conversion. Because ATPS are able to partition inhibitors (sugar) between the phases, the conversion rate can be maintained. However, phase forming components should be selected to preserve the enzymatic activity. The experimental results presented here contribute to a feasible ATPS-based conceptual process design for the enzymatic conversion of lignocellulosic material.

An Ionic Liquid-Based Biorefinery Approach for Duckweed Utilization

“…This is substantially faster than observed using more “traditional” lignocellulosic biomass such as miscanthus or sugarcane bagasse, which have been show to take around 24–72 h to reach full near-maximum yield under the similar enzyme loadings. 68 , 76 − 78 Such accelerated rates are extremely important, allowing for a potential trade-off between enzyme loading and hydrolysis duration. As pretreatment severity increased, hydrolysis kinetics tended to increase slightly, likely as a result of increased disruption of the biomass structure and, thus, higher substrate accessibility.…”

Renewable Carbohydrates: Advancements in Sustainable Glucose Production and Optimization

“…General outline of the process to produce glucose from sugarcane bagasse include preparation of Sugarcane Bagasse, pretreatment, enzymatic hydrolysis, fermentation, separation and purification and glucose concentration. Previous work done using the feedstock to produce glucose are found in Roslan & Salimi (2019), Lv et al (2022) and Bussamra et al (2020). Glucose produced from rice husk can find applications in various industries, similar to glucose produced from other lignocellulosic biomass sources.…”

Extraction of phytochemicals from date palm ( Phoenix dactylifera L.) seeds by enzymatic hydrolysis

“…Enzymatic hydrolysis (EH) has been used to produce sugar from different kinds of agricultural waste products that include rice hulls (Díaz et al, 2014), sugar cane bagasse (Bussamra et al, 2020), sweet sorghum (Pengilly et al, 2015), and wood residues (Álvarez et al, 2016). The most commonly used enzymes for carbohydrate hydrolysis are cellulases and amylases (Dashtban et al, 2009).…”