Enzymatic hydrolysis of steam-exploded sugarcane bagasse using high total solids and low enzyme loadings

Ramos, Luiz Pereira; Silva, Larissa da; Ballem, Annielly Comelli; Pitarelo, Ana Paula; Chiarello, Luana Marcele; Silveira, Marcos Henrique Luciano

doi:10.1016/j.biortech.2014.10.087

Cited by 97 publications

(44 citation statements)

References 28 publications

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“…Steam explosion has been proposed as one of the most efficient methods to deconstruct the plant cell wall macromolecular organization. [5][6][7][8] This process acts both chemically and physically by exposing the plant biomass to high pressure steam at temperatures ranging from 170 to 230 °C for reaction times varying from 2 to 30 min in the absence or presence of an exogenous acid or basic catalyst. When pretreatment is performed in the presence of an acid catalyst, such as in the case of sulfuric (H 2 SO 4 ) or phosphoric (H 3 PO 4 ) acids, the requirements for time and temperature are decreased considerably, depending on the acid strength and its actual concentration in relation to the biomass dry mass.…”

Section: Introductionmentioning

confidence: 99%

“…Also, hemicelluloses are almost completely removed and lignin is modified to a deeper extend, leading to cellulosic materials that are more susceptible to acid or enzymatic hydrolysis. 4,[6][7][8][9] The use of dilute H 3 PO 4 as a pretreatment catalyst, compared to strong acids such as H 2 SO 4 , results in lower sugar losses and less accumulation of furan compounds in the reaction medium. [10][11][12][13] Also, H 3 PO 4 is less corrosive and can act as an additional source of nutrients for microbial growth, particularly in the form of ammonium phosphate.…”

Section: Introductionmentioning

confidence: 99%

“…Cellic ® CTec2 corresponds to the second generation of these enzymes and many studies have already shown its superior performance for a variety of feedstocks, pretreatment technologies and process designs. 8,16 Ethanol can be produced from substrate hydrolysates by fermentation of pentoses (C5 fraction) and hexoses (C6 fraction). The efficient co-fermentation of these sugar streams is challenging because they follow different metabolic pathways that are rarely found together in naturally occurring microorganisms but developments in genetic and/or metabolic engineering have already achieved excellent results during the last decade or so.…”

Section: Introductionmentioning

confidence: 99%

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Ethanol Production from Sugarcane Bagasse Using Phosphoric Acid-Catalyzed Steam Explosion

Pitarelo¹,

Fonseca²,

Chiarello³

et al. 2016

Journal of the Brazilian Chemical Society

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The steam explosion was carried out in the absence (autohydrolysis) and presence of phosphoric acid to evaluate the effects of temperature (180 and 210 °C), acid concentration (0 and 19 mg g -1 , dry basis) and pretreatment time (5 and 10 min) on the structure and reactivity of sugarcane bagasse. Glucan recovery was used as the main response factor for pretreatment optimization through a central composite design. Autohydrolysis at 210 °C for 10 min had a good pretreatment performance but phosphoric acid catalysis (19 mg g -1 ) resulted in better yields under considerably milder conditions (180 °C, 5 min). Hydrolysis of both substrates for 96 h using 8 wt.% total solids and 30 mg g -1 Cellic ® CTec2 (Novozymes) provided total glucose yields of 75% in average. The production of cellulosic ethanol was assessed by both separate and simultaneous hydrolysis and fermentation using Saccharomyces cerevisiae. Freeze-drying of pretreatment water solubles reduced the concentration of furfural, hydroxymethylfurfural and acetic acid by more than 80% and this eliminated their inhibitory effect on yeast fermentation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethanol Production from Sugarcane Bagasse Using Phosphoric Acid-Catalyzed Steam Explosion

Pitarelo¹,

Fonseca²,

Chiarello³

et al. 2016

Journal of the Brazilian Chemical Society

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“…The condition ranges chosen were based on the other researcher's previous work. [14][15][16][17] The factors were constructed in half level factorial designs of 2 5-1 to screen their effect on the response of glucose production. The validation run for factorial analysis was carried by comparing the experimental values with the predicted model generated by Design Expert software.…”

Section: Experimental Designmentioning

confidence: 99%

Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6

Hashim

Yussof

Hong

et al. 2017

JPS

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“…Such production process depends on five sequential steps involving: (1) collection and preparation of plant biomass; (2) pretreatment for increasing the susceptibility of plant polysaccharides to bioconversion at high process yields; (3) enzymatic hydrolysis for converting plant polysaccharides into fermentable sugars; (4) microbial fermentation for cellulosic ethanol production; and, finally, (5) the recovery of ethanol by distillation [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Production of cellulosic ethanol from steam-exploded Eucalyptus urograndis and sugarcane bagasse at high total solids and low enzyme loadings

Chiarello

Ramos

Neves

et al. 2016

Sustain Chem Process

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Cellulosic ethanol is one of the most important biotechnological products to mitigate the consumption of fossil fuels and to increase the use of renewable resources for fuels and chemicals. By performing this process at high total solids (TS) and low enzyme loadings (EL), one can achieve significant improvements in the overall cellulosic ethanol production process. In this work, steam-exploded materials were obtained from Eucalyptus urograndis chips and sugarcane bagasse to be subsequently used for enzymatic hydrolysis at high TS (20 wt%) and relatively low EL (13.3 FPU g −1 TS of Cellic CTec3 from Novozymes). Also, the fermentability of their corresponding hydrolysates was tested using an industrial strain of Saccharomyces cerevisiae (Thermosacc Dry from Lallemand). Enzymatic hydrolysis of steam-treated E. urograndis reached 125 g L −1 of glucose in 72 h, while steam-treated bagasse gave yields 25 % lower. Both substrate hydrolysates were easily converted to ethanol, giving yields above 25 g L −1 and productivities of 2.3 g L −1 h −1 for eucalypt and 2.2 g L −1 h −1 for bagasse after only 12 h of fermentation. Under the conditions used in this study, sugarcane bagasse glucans showed the potential to boost the ethanol production from sugarcane culms by 31 %, from the 80 L t −1 of first generation to a total production of 105 L t −1. On the other hand, E. urograndis plantations are able to achieve cellulosic ethanol productivities of 2832.2 L ha −1 year −1 , which was 57.8 % higher than the projected value of 1794.5 L ha −1 year −1 that was obtained for sugarcane bagasse.

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Enzymatic hydrolysis of steam-exploded sugarcane bagasse using high total solids and low enzyme loadings

Cited by 97 publications

References 28 publications

Ethanol Production from Sugarcane Bagasse Using Phosphoric Acid-Catalyzed Steam Explosion

Ethanol Production from Sugarcane Bagasse Using Phosphoric Acid-Catalyzed Steam Explosion

Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6

Production of cellulosic ethanol from steam-exploded Eucalyptus urograndis and sugarcane bagasse at high total solids and low enzyme loadings

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