Enzymatic hydrolysis of pretreated rice straw

Vlasenko, Elena; Ding, Hanshu; Labavitch, John M.; Shoemaker, Sharon P.

doi:10.1016/s0960-8524(96)00169-1

Cited by 187 publications

(85 citation statements)

References 36 publications

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“…The results from different workers with a cellulase fraction of Trichoderma sp. (IMB-Tr) and Trichoderma reesei obtained 30% reducing sugars from alkali pretreated paddy straw 12 while Vlasenko et al(1997) showed the release of 37-46% sugars using 6.7% FPU/g of commercial Trichoderma reesei derived enzyme.…”

Section: Resultsmentioning

confidence: 99%

Paddy straw as substrate for ethanol production

2007

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Pretreatment of paddy straw with 2% sodium hydroxide at 15 psi for 1 h resulted in 83% delignifi cation. The hydrolysis of alkali treated paddy straw with a commercial preparation of cellulase for 2 h at 50°C resulted in release of 65% total reducing sugars. Maximum sugars were released at enzyme loading of 1.5% (v/v) .The fermentation of hydrolysate supplemented with nutrients by S. cerevisiae resulted in the production of 20-30 g L -1 ethanol after 48 h incubation which was further improved with addition of yeast nitrogen base and inoculated with 1% (w/v) yeast cells.

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Section: Resultsmentioning

confidence: 99%

Paddy straw as substrate for ethanol production

2007

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“…Vlasenko et al (1997) have found that after acid pretreatment (10% solid mater, 0.8% acid, 160°C) followed by enzyme treatment (cellulose 100 L + cellubiase) the yield of glucose was 43 g L −1 or 43% conversion of cellulose to glucose. The results in present study is slightly less than Valsenko et al (1997) reported.…”

Section: Discussionmentioning

confidence: 99%

Bioethanol Production from Rice Straw

Yoswathana¹,

Phuriphipat²,

Treyawutthiwat³

et al. 2010

Energy Research Journal

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Problem statement: Rice straw has been treated with different chemical (acid, alkali) and physical (subcritical water, ultrasound) methods to convert lignocellulose material to sugar. In addition enzyme treatment of pretreated samples has been applied to improve the conversion of lignocellulose material in sugar. Approach: Sulfuric acid at concentration of 1-9% was applied for acid treatment. For alkali treatment was sodium hydroxide solution at concentration of 1-5% used. Subcritical water treatment carried out at 160°C (5 bar) and 200°C (15 bar) for 10 min. Ultrasound was applied as combination method after acid pretreatment. The condition during ultrasound treatment was 40 W at 50°C and 10 min. Finally the pretreated sample was fermented using Saccharomyces cerevisiae yeast and the amount of produced ethanol was measured. Results: Acid treatment at 121°C, 15 min is an effective pretreatment method for converting lignocellulose to sugar. Up to 21.45% sugar w/w could be measure after acid treatment. Combination of chemical pretreatment and subsequent enzyme treatment increased the sugar yield drastically. Up to 37 and 28% sugar w/w could be achieved for acid and alkali pretreated samples respectively. Subcritical Water (SCW) treatment method is an effective physical method. SCW treatment at 200°C and 10 min followed by enzyme treatment yielded up to 17% sugar w/w. Combination of acid pretreatment with ultrasonic before enzyme treatment increased the conversion of lignocelluloses to sugar. Sugar yield up to 44% w/w after combination of acid and ultrasonic pretreatment and subsequent enzyme treatment could be achieved. Fermentation of pretreated rice straw shown that after 3 days fermentation most of sugar (55-65%) will be converted to bioethanol. The remaining sugar could not be converted in ethanol even after 6 days fermentation. Under these conditions, the maximum ethanol of 1.69% (v/v) was obtained. Conclusion: The combination method of acid pretreatment combined with ultrasound and subsequent enzyme treatment result the highest conversion of lignocelluse in rice straw to sugar and consequently, highest ethanol concentration after 6 days fermentation with S. cerevisae yeast.

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“…The biomass is exposed to liquid ammonia or CO 2 at high temperature and pressure for a short period of time, followed by a sudden drop in pressure. Unlike steam explosion, AFEX does not solubilize hemicelluloses (Vlasenko et al, 1997) but does require recovery of the ammonia for cost and environmental reasons (Sun and Cheng, 2002). CO 2 explosion is not as effective as AFEX or steam explosion (Dale and Moreira, 1982).…”

Section: Physico-chemical Pretreatmentmentioning

confidence: 99%