Enzymatic hydrolysis of microalgae proteins using serine proteases: A study to characterize kinetic parameters

Sedighi, Mehdi; Jalili, Hasan; Darvish, Maliheh; Sadeghi, Somayeh; Siadat, Seyed Omid Ranaei

doi:10.1016/j.foodchem.2019.01.111

Cited by 43 publications

(27 citation statements)

References 34 publications

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“…We report here the existence of AMPs from the microalgae T. suecica , with bactericidal effect against Gram− and Gram+ human pathogenic bacteria. The antibacterial properties of protein hydrolysates were described for the microalgae Dunaliella salina [35] and Chlorella sorokiniana [36], but the specific peptides responsible for this activity were not determined. Thus, to our knowledge, this is the first report of antimicrobial activity associated with peptides in microalgae.…”

Section: Discussionmentioning

confidence: 99%

Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement

et al. 2019

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The outburst of microbial resistance to antibiotics creates the need for new sources of active compounds for the treatment of pathogenic microorganisms. Marine microalgae are of particular interest in this context because they have developed tolerance and defense strategies to resist the exposure to pathogenic bacteria, viruses, and fungi in the aquatic environment. Although antimicrobial activities have been reported for some microalgae, natural algal bioactive peptides have not been described yet. In this work, acid extracts from the microalga Tetraselmis suecica with antibacterial activity were analyzed, and de novo sequences of peptides were determined. Synthetic peptides and their alanine and lysine analogs allowed identifying key residues and increasing their antibacterial activity. Additionally, it was determined that the localization of positive charges within the peptide sequence influences the secondary structure with tendency to form an alpha helical structure.

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Section: Discussionmentioning

confidence: 99%

Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement

et al. 2019

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“…In that concern, anticancer bioactive peptides have attracted researchers with their multifunctional potency, sensitivity and stability, and they have shown beneficial activities with the presence of carotenoids, flavonoids, phenolic acids and so on. However, some publications reported that the anticancer peptides from the enzymatic hydrolysis preparation of natural or synthetic peptides derived from food protein sources provide diverse bioactive components, which show anticancer activities [58,59]. A few studies have reported the cytotoxicity on human cancer cells using marine algae-derived peptides, as shown in Table 2.…”

Section: Anticancer Microalgal Peptidesmentioning

confidence: 99%

A Systemic Review on Microalgal Peptides: Bioprocess and Sustainable Applications

et al. 2021

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Nowadays, microalgal research is predominantly centered on an industrial scale. In general, multipotent bioactive peptides are the advantages over focal points over utilitarian nourishment as well as nutraceuticals. Microalgal peptides are now profoundly connected with biological properties rather than nutritive. Numerous techniques are employed to purify active peptides from algal protein using enzymatic hydrolysis; it is broadly used for numerous favorable circumstances. There is a chance to utilize microalgal peptides for human well-being as nutritive enhancements. This exhaustive survey details the utilization of microalgal peptides as antioxidant, anti-cancerous, anti-hypersensitive, anti-atherosclerotic, and nutritional functional foods. It is also exploring the novel technologies for the production of active peptides, for instance, the use of algal peptides as food for human health discovered restrictions, where peptides are sensitive to hydrolysis protease degradation. This review emphasizes the issue of active peptides in gastrointestinal transit, which has to be solved in the future, and prompt impacts.

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“…Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme as the trend to use natural food as an additive (Sadeghi et al, 2019). Enzymatic hydrolysis is preferred over chemical hydrolysis since the process can be controlled, and the final product can be further developed (Iltchenco et al, 2017).…”

Section: Extraction Of Collagen From the Skin Of Kacang Goat And Production Of Itsmentioning

confidence: 99%

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Hakim

Pratiwi

Jamhari

et al. 2021

Trop. Anim. Sci. J.

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The study was designed to determine the potential of collagen hydrolysate produced from the skin of Kacang goat through chymotrypsin hydrolysis to be used as an inhibitor of angiotensin converting enzyme (ACE). This research was conducted in three replications, with the measured parameters include ACE inhibitory potential and collagen hydrolysate fractionation. The results showed that collagen extraction of Kacang goat skin by chymotrypsin hydrolysis yielded 9.74% (dry matter, v/v) collagen, with pH at 6.6. The extracted collagen contained α1, α2, and β collagen chains with molecular weights of 151 kDa, 141 kDa, and 240 kDa, respectively. Furthermore, the collagen hydrolysis produced protein peptides confirmed at molecular weights of 43 to 107 kDa. The hydrolysate fractionation at molecular weights of <3 kDa, 3-5 kDa, and >5 kDa showed proteins concentrations of 2.33 mg/mL, 3.81 mg/mL, and 3.93 mg/mL, respectively. The hydrolysate fractionation with molecular weight <3 kDa showed to have ACE inhibition activity with the IC50 value of 0.47 mg/mL. The study concluded that collagen hydrolysate extracted from the skin of Kacang goat had a promising potential as a source of antihypertensive agent.

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Enzymatic hydrolysis of microalgae proteins using serine proteases: A study to characterize kinetic parameters

Cited by 43 publications

References 34 publications

Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement

Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement

A Systemic Review on Microalgal Peptides: Bioprocess and Sustainable Applications

Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

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