Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina

Jagtap, Sujit Sadashiv; Dhiman, Saurabh Sudha; Kim, Tae Su; Li, Jinglin; Lee, Jung-Kul

doi:10.1016/j.biortech.2013.01.118

Cited by 31 publications

(13 citation statements)

References 26 publications

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“…Strain maintenance and enzyme production and characterization P. adiposa SKU714 and A. gemina KJS114 were isolated from soil samples taken from Sorak Mountain, the Republic of Korea. The isolation and identification of the strains were performed as described by previous studies [8,9]. The identified strains were deposited at the Korean Culture Center of Microorganisms (KCCM) under the accession numbers 11187P (P. adiposa SKU714) and 11186P (A. gemina KJS114 KCCM).…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, a thermochemical pretreatment was applied, using 2% NaOH over a range of temperatures from 80°C to 100°C [9]. Before the pretreatment, the canola biomass contained 34.4% cellulose, 29% lignin, and 20.1% hemicelluloses; after pretreatment, it contained 46.9% cellulose, 10.2% lignin, and 8.3% hemicelluloses.…”

Section: Thermochemical Pretreatment Of Biomassmentioning

confidence: 99%

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Phytoremediation of metal-contaminated soils by the hyperaccumulator canola (Brassica napus L.) and the use of its biomass for ethanol production

Dhiman

Selvaraj

et al. 2016

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Section: Methodsmentioning

confidence: 99%

Section: Thermochemical Pretreatment Of Biomassmentioning

confidence: 99%

Phytoremediation of metal-contaminated soils by the hyperaccumulator canola (Brassica napus L.) and the use of its biomass for ethanol production

Dhiman

Selvaraj

et al. 2016

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“…Sin embargo, lo anterior puede ocasionar un aumento en la concentración de subproductos inhibidores del proceso fermentativo como furfural y 5-hidroximetilfurfural (Saha et al 2005, Camesasca et al 2015, Martínez et al 2015. El contenido de lignina soluble se midió en el licor resultante del PT2 debido a que la lignina se solubiliza en solución alcalina acuosa (Jagtap et al 2013). Las condiciones que generaron mayor pérdida de lignina corresponden al 3 % de NaOH (57.07 % calculado a partir del % ASL).…”

Section: Pretratamientounclassified

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

Rodríguez

Castrillo

Velázquez

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: residuo lignocelulósico, diseño factorial, lignina, sacarificación. RESUMENEl uso de residuos lignocelulósicos constituye una fuente abundante y de bajo costo, que genera, además, una solución al problema de su disposición final. El objetivo del trabajo fue evaluar el efecto de distintas condiciones de pretratamiento secuencial ácido-base de aserrín de pino, sobre la liberación de azúcares reductores en la etapa de sacarificación. El material lignocelulósico se pretrató en dos etapas: la primera, se realizó con ácido sulfúrico (PT1) y la segunda con hidróxido de sodio (PT2), ambas con tres niveles: 1, 2 y 3 % mediante un diseño 3 2 factorial. La hidrólisis enzimática se realizó a 50 ºC y 150 rpm en frascos de 50 mL. Se tomaron muestras a las 0.15, 24, 48, 72 y 96 h. Se determinó el porcentaje de lignina ácido soluble y la concentración de azúcares reductores. El nivel óptimo de cada factor que favoreció la mayor deslignificación correspondió a 3 % de ácido sulfúrico y 3 % de hidróxido de sodio. La producción de azúcares reductores no se vio afectada por el PT2, pero sí por el PT1, alcanzando un máximo para el nivel 0 del mismo (2 % de ácido sulfúrico). Se optimizó el pretratamiento secuencial ácido-base y se comprobó además que éste mejora la hidrólisis enzimática respecto al aserrín de pino sin pretratar. La cantidad de azúcares reductores obtenidos fue comparable a la obtenida por otros autores.Key words: lignocellulosic waste, factorial design, lignin, saccharification. ABSTRACTThe use of lignocellulosic waste constitutes an abundant and inexpensive source; it also offers a solution to the problem of waste disposal. The aim of the study was to evaluate the effect of different conditions of a sequential acid-base pretreatment of pine sawdust, through the release of reducing sugars in the saccharification step. The lignocellulosic material was pretreated in two stages: acid pretreatment (PT1), using sulfuric acid and basic pretreatment (PT2), employing sodium hydroxide at three levels each one: 1, 2 and 3 % in a factorial design. Enzymatic hydrolysis was carried out at 50 ºC and 150 rpm in 50 mL vials. Samples were taken at 0.15, 24, 48, 72 and 96 h. The percentage of acid soluble lignin and the concentration of reducing sugars were determined. The Rev. Int.

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“…However, a mixture of cellulase components from different origins, generally named as cellulase cocktail, brings about higher activities. CBH I and CBH II have a strong synergism in solubilizing microcrystalline cellulose (MCC) [13], and CBH and EG, or CBH, EG, and BGL are capable to make a stronger synergism when degrading MCC and soluble cellulose [14,15]. In addition, cellulosomes from some of anaerobic bacteria and fungi provide a high synergism in hydrolyzing MCC but show no function when encountered by soluble cellulose [16].…”

Section: Introductionmentioning

confidence: 98%

A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

Zhao

Wang

Tong

et al. 2015

Appl Biochem Biotechnol

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Compared to Trichoderma reesei RUT-C30 cellulase (Trcel), Penicillium oxalicum 16 cellulase (P16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (MCC), possessed a 10-fold higher β-glucosidase (BGL) activity, but obtained somewhat lower other cellulase component activities. The optimal temperature and pH of β-1,4-endoglucanase, cellobiohydrolase, and filter paperase from P16cel were 50-60 °C and 4-5, respectively, but those of BGL reached 70 °C and 5. The cellulase cocktail of P16cel and Trcel had a high synergism when solubilizing MCC and generated 1.7-fold and 6.2-fold higher glucose yields than P16cel and Trcel at the same filter paperase loading, respectively. Additional low concentration of fructose enhanced the glucose yield during enzymatic hydrolysis of MCC; however, additional high concentration of monosaccharide (especially glucose) reduced cellulase activities and gave a stronger monosaccharide inhibition on Trcel. These results indicate that P16cel is a more excellent cellulase than Trcel.

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Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina

Cited by 31 publications

References 26 publications

Phytoremediation of metal-contaminated soils by the hyperaccumulator canola (Brassica napus L.) and the use of its biomass for ethanol production

Phytoremediation of metal-contaminated soils by the hyperaccumulator canola (Brassica napus L.) and the use of its biomass for ethanol production

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

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