Enzymatic hydrolysis by Humicola lanuginosa lipase of polycaprolactone monolayers

Grozev, Nikolay A.; Svendsen, Allan; Verger, R.; Panaı̈otov, I.

doi:10.1007/s003960200001

Cited by 16 publications

(23 citation statements)

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“…The system of differential equations which correspond to the proposed kinetic model are solved for the steady state and presented elsewhere [8][9][10]. Here, we give the final solutions for the surface concentrations C S and C P .…”

Section: Interfacial Hydrolysis Kinetic Modelmentioning

confidence: 99%

“…For the interpretation of the experimental data DA(t), we have followed the theoretical approach thoroughly developed in [8][9][10]. Accordingly, the decrease in the monolayer area is considered as a direct result from the solubilization of the progressively appearance at the interface soluble peptide residues as a result of the fragmentation of the chain A.…”

Section: Interpretation Of the Obtained Experimental Data Da(t)mentioning

confidence: 99%

“…The Langmuir technique has proven its advantage to control over the interfacial quality of the surface by means of surface pressure and monolayer composition. The method has been successfully utilized for variety of substrate (monolayer)-enzyme systems, for example-Dipalmytoilphosphatidylcholine (DPPC)-phospholipase A 2 (PLA 2 ) in the presence of b-cyclodextrin [6], poly(D-L-lactide)-cutinase [7], poly (lactic acid)-poly (glycolic acid)-Humicola Lanuginose Lipase (HLL) [8], polycaprolactone-Humicola Lanuginose Lipase (HLL) monolayers [9], alpha gliadin-Savinase [10]. The physicochemical characterization of these substrate-enzyme systems has been achieved by measuring the surface parameters -surface pressure, surface potential, and molecular area during the course of the occurred enzyme reactions.…”

Section: Introductionmentioning

confidence: 99%

“…In order to analyze the mechanisms of degradation of insulin molecules, a previously developed kinetic model for alpha gliadin-Savinase system [8][9][10] was fitted to the obtained experimental data. The measured decrease in the monolayer area versus time is a direct result from the random scission of the peptide bonds leading to the progressive fragmentation of the polypeptide chain and a subsequent solubilization of the amino acid residues in the adjacent aqueous phase.…”

Section: Introductionmentioning

confidence: 99%

“…In principle, the polymer monolayers at the air-water interface and in particular, protein monolayers are substrates for the enzyme action of the proteases suitably organized at a molecular level [8][9][10] . Once formed at the interface, the monolayer represents structurally well defined and interfacially controlled substrate for the enzyme action.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging

Balashev

Ivanova

Mircheva

et al. 2011

Journal of Colloid and Interface Science

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Section: Interfacial Hydrolysis Kinetic Modelmentioning

confidence: 99%

Section: Interpretation Of the Obtained Experimental Data Da(t)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging

Balashev

Ivanova

Mircheva

et al. 2011

Journal of Colloid and Interface Science

Self Cite

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Enzymatic Degradation of Oligo(ε‐caprolactone)s End‐Capped with Phenylboronic Acid Derivatives at the Air–Water Interface

Roßberg

Rottke

Schulz

et al. 2016

Macromol. Rapid Commun.

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The influence of terminal functionalization of oligo(ε-caprolactone)s (OCL) with phenylboronic acid pinacol ester or phenylboronic acid on the enzymatic degradation behavior at the air-water interface is investigated by the Langmuir monolayer degradation technique. While the unsubstituted OCL immediately degrades after injection of the enzyme lipase from Pseudomonas cepacia, enzyme molecules are incorporated into the films based on end-capped OCL before degradation. This incorporation of enzymes does not inhibit or suppress the film degradation, but retards it significantly. A specific binding of lipase to the polymer monolayer allows studying the enzymatic activity of bound proteins and the influence on the degradation process. The functionalization of a macromolecule with phenyl boronic acid groups is an approach to investigate their interactions with diol-containing biomolecules like sugars and to monitor their specified impact on the enzymatic degradation behavior at the air-water interface.

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Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

Machatschek

Schulz

Lendlein

2018

Macromol. Rapid Commun.

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Langmuir monolayers provide a fast and elegant route to analyze the degradation behavior of biodegradable polymer materials. In contrast to bulk materials, diffusive transport of reactants and reaction products in the (partially degraded) material can be neglected at the air–water interface, allowing for the study of molecular degradation kinetics in experiments taking less than a day and in some cases just a few minutes, in contrast to experiments with bulk materials that can take years. Several aspects of the biodegradation behavior of polymer materials, such as the interaction with biomolecules and degradation products, are directly observable. Expanding the technique with surface‐sensitive instrumental techniques enables evaluating the evolution of the morphology, chemical composition, and the mechanical properties of the degrading material in situ. The potential of the Langmuir monolayer degradation technique as a predictive tool for implant degradation when combined with computational methods is outlined, and related open questions and strategies to overcome these challenges are pointed out.

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Enzymatic hydrolysis by Humicola lanuginosa lipase of polycaprolactone monolayers

Cited by 16 publications

References 0 publications

Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging

Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging

Enzymatic Degradation of Oligo(ε‐caprolactone)s End‐Capped with Phenylboronic Acid Derivatives at the Air–Water Interface

Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

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