Enzymatic-HPLC Method to Analyze<scp>D</scp>-3-Hydroxybutyric Acid in Rat Serum

Ishii, Takashi

doi:10.1271/bbb.100167

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…The serum concentration of β-hydroxybutyrate was analyzed by HPLC. (15) Liver triacylglycerol, (16) phospholipid (17) and cholesterol (18) concentrations were determined as described previously. The hepatic concentration of carnitine was analyzed by the method of Pearson et al (19) Serum and liver concentrations of sesamin and episesamin were analyzed by HPLC as described previously.…”

Section: Methodsmentioning

confidence: 99%

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

Ishii

2014

J. Clin. Biochem. Nutr.

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We studied the combined effect of sesamin (1:1 mixture of sesamin and episesamine) and soybean phospholipid on lipid metabolism in rats. Male rats were fed diets supplemented with 0 or 2 g/kg sesamin, and containing 0 or 50 g/kg soybean phospholipid, for 19 days. Sesamin and soybean phospholipid decreased serum triacylglycerol concentrations and the combination of these compounds further decreased the parameter in an additive fashion. Soybean phospholipid but not sesamin reduced the hepatic concentration of triacylglycerol. The combination failed to cause a strong decrease in hepatic triacylglycerol concentration, presumably due to the up-regulation of Cd36 by sesamin. Combination of sesamin and soybean phospholipid decreased the activity and mRNA levels of hepatic lipogenic enzymes in an additive fashion. Sesamin strongly increased the parameters of hepatic fatty acid oxidation enzymes. Soybean phospholipid increased hepatic activity of 3-hydroxyacyl-CoA dehydrogenase although it failed to affect the activity of other enzymes involved in fatty acid oxidation. Sesamin strongly increased hepatic concentration of carnitine. Sesamin and soybean phospholipid combination further increased this parameter, accompanying a parallel increase in mRNA expression of carnitine transporter. These changes can account for the strong decrease in serum triacylglycerol in rats fed a diet containing both sesamin and soybean phospholipid.

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Section: Methodsmentioning

confidence: 99%

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

Ishii

2014

J. Clin. Biochem. Nutr.

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Combined effect of sesamin and α-lipoic acid on hepatic fatty acid metabolism in rats

et al. 2012

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Sesamin and α-lipoic acid decreased serum lipid concentrations and the combination of these compounds further decreased the parameters in an additive fashion. These compounds reduced the hepatic concentration of triacylglycerol, the lignan being less effective in decreasing this value. The combination failed to cause a stronger decrease in hepatic triacylglycerol concentration. The combination of sesamin and α-lipoic acid decreased the activity and mRNA levels of hepatic lipogenic enzymes in an additive fashion. Sesamin strongly increased the parameters of hepatic fatty acid oxidation enzymes. α-Lipoic acid antagonized the stimulating effect of sesamin of fatty acid oxidation through reductions in the activity of some fatty acid oxidation enzymes and carnitine concentration in the liver. This may account for the failure to observe strong reductions in hepatic triacylglycerol concentration in rats given a diet containing both sesamin and α-lipoic acid.

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Physiological activities of the combination of fish oil and α-lipoic acid affecting hepatic lipogenesis and parameters related to oxidative stress in rats

Ishii

2017

Eur J Nutr

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Enzymatic-HPLC Method to AnalyzeD-3-Hydroxybutyric Acid in Rat Serum

Cited by 4 publications

References 17 publications

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

Combined effect of sesamin and α-lipoic acid on hepatic fatty acid metabolism in rats

Physiological activities of the combination of fish oil and α-lipoic acid affecting hepatic lipogenesis and parameters related to oxidative stress in rats

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