Enzymatic fuel cells: Integrating flow-through anode and air-breathing cathode into a membrane-less biofuel cell design

Rincón, Rosalba A.; Lau, Carolin; Luckarift, Heather R.; Garcia, Kristen; Adkins, Emily; Johnson, Glenn R.; Atanassov, Plamen

doi:10.1016/j.bios.2011.06.029

Cited by 104 publications

(44 citation statements)

References 30 publications

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“…Recently, Cosnier et al followed the same route to induce porosity in a polymer film. [251] Many other works report the mixture of different materials to modulate the porosity for electroenzymatic reactions, increasing at the same time the conductivity of the material: Ag NP and CNTs, [252] reticulated vitreous carbon mixed with CNTs, [253] and CFs dipped in CNT dispersions. [209,254,255] Catalytic currents were increased by the addition of CNTs, but, surprisingly, some discrepancy on DET or MET processes exist between the works.…”

Section: A Challenging Issue: Overcoming Mass-transport Limitationmentioning

confidence: 99%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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Among sustainable alternatives to fossil fuel, proton exchange membrane fuel cells are promising devices that deliver electricity from hydrogen and oxygen, producing only water. The transformation of the fuel and oxidant however relies on rare‐metal catalysts. H2/O2 enzymatic biofuel cells thus emerge as sustainable biotechnological devices in which chemical catalysts are replaced by hydrogenases at the anode and multicopper oxidases at the cathode. This review discusses the recent breakthroughs and the limitations in all the components of H2/O2 biofuel cells: 1) Catalytic mechanisms involved in multicopper oxidases and hydrogenases, in addition to the new potential of enzymes from the biodiversity pool, which exhibit outstanding properties. 2) The molecular basis for oriented enzyme immobilization on the electrochemical interfaces as a prerequisite for a fast direct electron‐transfer rate. 3) The requirement for 3D networks to enhance current densities. 4) The production of H2 from biomass. 5) The history of H2/O2 biofuel cells and recent devices, which also highlights the remaining issues that will allow the use of such devices in low‐power applications.

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Section: A Challenging Issue: Overcoming Mass-transport Limitationmentioning

confidence: 99%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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“…The laccase reduction of dioxygen to water in the presence of different redox mediators or nanomaterials [28] has been studied in other electrochemical applications [29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Laccase biosensor based on screen-printed electrode modified with thionine–carbon black nanocomposite, for Bisphenol A detection

Portaccio

Tuoro

Arduini

et al. 2013

Electrochimica Acta

139

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a b s t r a c tThe relevance of Bisphenol A (BPA) in human health is well-known. For this reason we designed and developed a biosensor based on a bionanocomposite (laccase-thionine-carbon black)-modified screenprinted electrode. Thionine, a commercially available dye, was used as electrochemical mediator coupled with a nanostructured carbon black. By means of cyclic voltammetry, the interaction of thionine adsorbed on modified screen printed electrode with laccase/BPA reaction products has been studied. In addition, the immobilization of laccase by physical adsorption on the surface of thionine-carbon black modified screen printed electrodes was investigated. The response of the biosensor has been optimized in terms of enzyme loading, pH and applied potential reaching a linear concentration range of 0.5-50 M, a sensitivity of 5.0 ± 0.1 nA/M and a limit-of-detection (LOD) of 0.2 M. The developed biosensor has been also challenged in tomato juice samples contained in metallic cans where release of BPA due to the epoxy resin coating can be assumed. A satisfactory recovery value comprised between 92% and 120% was obtained.

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“…Despites its low-proton conductivity at the operative conditions of typical EFC devices (near neutral pH and 25 B T B 40°C), Nafion is still the most used electrolyte. Due to the high ionic resistance of the Nafion membrane, the use of membrane-less EFCs has also been explored [17,18], whereas only few reports have addressed the use of different polymer electrolyte alternative to Nafion [19]. Although recent research progress, EFC technology is still so far from being commercially spread, so that new materials and components must be developed and properly combined to maximize EFC final performance.…”

Section: Introductionmentioning

confidence: 99%

Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

et al. 2012

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We fabricated an enzyme fuel cell (EFC) device based on glucose as fuel and glucose oxidase (GOx) as biocatalyst. As a strategy to improve GOx stability, preserving at the same time the enzyme catalytic activity, we propose an immobilization procedure to entrap GOx in a polymer matrix based on Nafion and multiwalled carbon nanotubes. Circular dichroism (CD) spectra were recorded to study changes in the 3D structure of GOx that might be generated by the immobilization procedure. The comparison between the CD features of GOx immobilized and free in solution indicates that the shape of the spectra and position of peaks do not significantly change. The bioelectrocatalytic activity toward glucose oxidation of immobilized GOx was studied by cyclic voltammetry and chronoamperometry experiments. Such electrochemical experiments allow monitoring the rate of GOx-catalyzed glucose oxidation and extrapolating GOx kinetic parameters. Results demonstrate that immobilized GOx has high catalytic efficiency, due the maintaining of regular and well-ordered structure of the immobilized enzyme, as indicated by spectroscopic findings. Once investigated the electrode structure-property relationship, an EFC device was assembled using the GOx-based bioanode, and sulfonated poly ether ether ketone as electrolyte membrane.Polarization and power density curves of the complete EFC device were acquired, demonstrating the suitability of the immobilization strategy and materials to be used in EFCs.

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Enzymatic fuel cells: Integrating flow-through anode and air-breathing cathode into a membrane-less biofuel cell design

Cited by 104 publications

References 30 publications

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Laccase biosensor based on screen-printed electrode modified with thionine–carbon black nanocomposite, for Bisphenol A detection

Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

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Enzymatic fuel cells: Integrating flow-through anode and air-breathing cathode into a membrane-less biofuel cell design

Cited by 104 publications

References 30 publications

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Laccase biosensor based on screen-printed electrode modified with thionine–carbon black nanocomposite, for Bisphenol A detection

Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

Contact Info

Product

Resources

About

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective