Enzymatic esterification of eugenol and benzoic acid by a novel chitosan-chitin nanowhiskers supported Rhizomucor miehei lipase: Process optimization and kinetic assessments

Manan, Fatin Myra Abd; Attan, Nursyafreena; Zakaria, Zainoha; Keyon, Aemi Syazwani Abdul; Wahab, Roswanira Abdul

doi:10.1016/j.enzmictec.2017.09.004

Cited by 42 publications

(25 citation statements)

References 60 publications

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“…Literature has shown that this additional treatment transforms the lipase into a 'frozen' active state (open conformation) on the surface of the support [14]. Our observation is in agreement with similar studies of earlier researchers [34][35][36]. Subsequent protein loadings were lower as the supports were not pretreated with toluene.…”

Section: Effect Of Glutaraldehyde Concentrationsupporting

confidence: 91%

“…Toluene molecules bind to the hydrophobic lid of CRL and trigger the lid to open. This facilitates access of the substrates into the active site of the lipase, thereby resulting in higher lipase activity and immobilization efficiency [36]. The outcome seen in this study is consistent with the findings reported in the literature [37][38] for lipase immobilization that also uses a hydrophobic solvent to activate the lipase.…”

Section: Effect Of Glutaraldehyde Concentrationsupporting

confidence: 90%

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Effect of Glutaraldehyde Concentration on Catalytic Efficacy of Candida rugosa Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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Till date, studies that investigated the effect of glutaraldehyde concentration on catalytic efficacy of biocatalyst developed with silica-derived from oil palm leaves (OPL) as support, are unknown. The study presents the preparation of a support consisting of silica extracted from OPL coated over magnetite (G/A/SiO2-M) for the immobilization of Candida rugosa lipase (CRL). Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. Fourier transform infrared (FTIR) spectra and immobilization parameters indicated that covalent bound CRL on functionalized OPL-derived silica-magnetite composite activated with 4% (v/v) glutaraldehyde solution (100 mM, pH 7.0) (CRL/G/A/SiO2-M) and pretreated in toluene, resulted in a protein loading and an immobilization yield of 68.3 mg/g and 74.3%, respectively. The resultant CRL/G/A/SiO2-M biocatalyst which specific activity was 61.9 U/g catalyzed the esterification production of 76.5% butyl butyrate in just 3 h, as confirmed by analyses of the purified ester using FTIR and 1H NMR spectroscopy. Hence, the finding envisages the promising use of G/A/SiO2-M support fabricated from discarded OPL as a carrier for immobilization and activation of CRL, in conjunction to being a good alternative source of renewable silica.

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Section: Effect Of Glutaraldehyde Concentrationsupporting

confidence: 91%

Section: Effect Of Glutaraldehyde Concentrationsupporting

confidence: 90%

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of Candida rugosa Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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“…This is because the temperature threshold was exceeded, hence the surplus breaking of the ionic, intramolecular hydrogen bonds, van der Waals forces and hydrophobic interactions that stabilizes the enzyme tertiary structure is heightened. These changes increase structural over-flexibility and disrupt the active conformation of the enzyme which lower activity of DehSN1 (Manan et al, 2018). On contrary, dehalogenase activity of DehSN1 was at its lowest at 50 °C (with enzyme specific activity 0.061 µmol CI¯/min/mg), which is probably because of the thermal deactivation of the DehSN1 dehalogenase protein structure.…”

Section: Characterization Of Dehsn1mentioning

confidence: 99%

Purification and characterization of dehalogenase from Bacillus cereus SN1 isolated from cow dung

Ismail¹,

Edbeib²,

Seman³

et al. 2018

MJM

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This study was aimed to characterize a dehalogenase derived from Bacillus cereus SN1 isolated from cow dung. Methodology and results: Cell-free extract of Bacillus cereus SN1 was purified using ion exchange and gel filtration chromatography. Fraction B2 of gel filtration gave the highest enzyme specific activity (0.155 μmol CI¯/min/mg). The results of SDS-PAGE showed the enzyme was 25 kDa in size. The enzyme reached its optimum activity at 30 °C at pH 6, and was inhibited by Mercury(II) sulfate (HgSO4). The Km and kcat values were 0.2 mM and 1.22/sec, respectively. The partial dehalogenase gene sequence was amplified using Group I dehalogenase primers. The amplified gene sequence was designated as DehSN1. Conclusion, significance and impact of study: Dehalogenase from Bacillus cereus strain SN1 revealed new characteristics of dehalogenase protein. The findings indicated that the DehSN1 dehalogenase is a promising candidate for further studies as a bioremediation agent for agricultural applications.

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“…Suitability of this mathematical and statistical tool has been established in a myriad of experimental trials for different processes. In fact, the BBD has been proven excellent for evaluating and observing influences, as well as interactions of multiple factors in any given process; benefits that are unseen in the one-variable-at-a-time technique [26][27]. Thus, the objective of this study was to seek the best aforementioned UAE conditions for maximizing the extraction yield and total phenolic content of E. guineensis leaves extract.…”

Section: ■ Introductionmentioning

confidence: 99%

Statistical Modelling of Ultrasonic-Aided Extraction of Elaeis guineensis Leaves for Better-Quality Yield and Total Phenolic Content

et al. 2019

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The present study highlighted the statistical modeling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time (X2: 5−55 min), the solvent-to-solid ratio (X3: 15:1−35:1 mL/g) and sonification amplitude (X4: 20−100 %). Under optimized conditions, the highest EY of 14.38% was attained using 50% (v/v) ethanol:water ratio, 55 min, 35 mL/g solvent-to-solid ratio, 60% sonication amplitude, whereas maximum TPC was 209.70 mg gallic acid equivalent (GAE)/g [50% (v/v) ethanol:water ratio, 30 min, 25 mL/g solvent-to-solid ratio, 60% sonication amplitude]. Second-order polynomial models of EY and TPC showed the R2 value corresponding to 0.9303 and 0.9500, respectively, indicating their significance (p < 0.05) to predict the responses. HPLC chromatograms revealed gallic acid and catechin were present in the UAE extracts. UAE technique afforded better EY (14.38%) and TPC (209.70 mg GAE/g) than maceration (3.73%, 85.23 mg GAE/g) and Soxhlet (6.86%, 102.13 mg GAE/g) extractions, as based on scanning electron micrographs of untreated, UAE, macerated and Soxhlet treated samples. Cell walls of ultrasonic-treated E. guineensis leaves were visibly disrupted to facilitate the higher release of bioactive plant materials, thus justifying the higher EY and TPC. The application of ultrasound appeared to remarkably increase the extraction efficiency of E. guineensis leaves to extract as compared to the conventional methods.

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Enzymatic esterification of eugenol and benzoic acid by a novel chitosan-chitin nanowhiskers supported Rhizomucor miehei lipase: Process optimization and kinetic assessments

Cited by 42 publications

References 60 publications

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Purification and characterization of dehalogenase from Bacillus cereus SN1 isolated from cow dung

Statistical Modelling of Ultrasonic-Aided Extraction of <i>Elaeis guineensis</i> Leaves for Better-Quality Yield and Total Phenolic Content

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