Enzymatic electrochemical biosensor for glyphosate detection based on acid phosphatase inhibition

Butmee, Preeyanut; Tumcharern, Gamolwan; Songsiriritthigul, Chomphunuch; Durand, Marie-José; Thouand, Gérald; Kerr, Margaret E.; Kalcher, Kurt; Samphao, Anchalee

doi:10.1007/s00216-021-03567-2

Cited by 17 publications

(16 citation statements)

References 40 publications

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“…In recent years, several studies have focused on detecting glyphosate residue in the soil. In these methods, the recognition units are mainly acid phosphatase [ 93 ], MIP [ 94 ], antibody [ 95 , 96 ], and aptamer [ 97 , 98 ]. Electrochemical spectroscopy, fluorescence, SERS, and electrophoresis can be used to detect glyphosate.…”

Section: Application Of Nanoparticle-based Sensors For Different Pest...mentioning

confidence: 99%

Recent Advances in Nanoparticle-Based Optical Sensors for Detection of Pesticide Residues in Soil

et al. 2023

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The excessive and unreasonable use of pesticides has adversely affected the environment and human health. The soil, one of the most critical natural resources supporting human survival and development, accumulates large amounts of pesticide residues. Compared to traditional spectrophotometry analytical methods, nanoparticle-based sensors stand out for their simplicity of operation as well as their high sensitivity and low detection limits. In this review, we focus primarily on the functions that various nanoparticles have and how they can be used to detect various pesticide residues in soil. A detailed discussion was conducted on the properties of nanoparticles, including their color changeability, Raman enhancement, fluorescence enhancement and quenching, and catalysis. We have also systematically reviewed the methodology for detecting insecticides, herbicides, and fungicides in soil by using nanoparticles.

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Section: Application Of Nanoparticle-based Sensors For Different Pest...mentioning

confidence: 99%

Recent Advances in Nanoparticle-Based Optical Sensors for Detection of Pesticide Residues in Soil

et al. 2023

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“…In literature, the most commonly described techniques for its determination are gas chromatography, ion chromatography, high-performance liquid chromatography (HPLC), chromatography–mass spectrometry, capillary electrophoreses, and enzyme-linked immunosorbent assay (ELISA) . Despite their selectivity and sensitivity, most of its determination procedures are expensive or require laborious sample derivatization. , Enzyme-immobilization-based electrochemical sensors have also been reported. , However, factors such as enzyme inhibition due to metal ion interferents, change of pH, and limited stability at varying temperatures restrict their widespread use . Electrochemical nonenzymatic sensors can offer stable, cost-effective, and rapid responses to analyte determination. , The current study has attained this robustness by adopting a two-dimensional carbon-based catalyst and a single-step sensor fabrication for its determination.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 Enzyme-immobilization-based electrochemical sensors have also been reported. 20,21 However, factors such as enzyme inhibition due to metal ion interferents, change of pH, and limited stability at varying temperatures restrict their widespread use. 22 Electrochemical nonenzymatic sensors can offer stable, cost-effective, and rapid responses to analyte determination.…”

Section: Introductionmentioning

confidence: 99%

A Non-Enzymatic Electrochemical Sensor for Glyphosate Adopting Surface Modified Screen-Printed Electrodes

Janjani

Bhardwaj

Kushwaha

2023

ACS Appl. Eng. Mater.

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In this study, a lab-on-chip electrochemical sensor was developed for ultratrace determination of glyphosate. A singlestep approach was adopted for the surface modification of screenprinted electrodes using reduced graphene oxide. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to characterize the electrochemical behavior of the modified electrode. The modified electrode exhibited lowered electron transfer resistance, improved current response, and demonstrated selectivity and sensitivity toward glyphosate. Differential pulse voltammetry was employed to quantify glyphosate. The detection limit for glyphosate was achieved up to 0.144 nmol L −1 for a wide concentration range of 1 to 1000 nM. The proposed sensor presents the advantage of nonenzymatically and specifically determining glyphosate and offers exceptional stability with a relative standard deviation of 3.3% for 10 days. Finally, the applicability of the developed technique was assessed by quantification of glyphosate in tap water and garden soil samples. The ease of fabrication and implementation of the proposed sensor can contribute to the quest for on-site determination of the ecological contaminants.

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“…We contend that carbon‐based materials exhibit promising electrical properties, electrocatalytic sites, large specific surface area/porosity, and low cost that is well‐suited for in‐field environmental sensing. [ 36 , 37 , 38 ] Researchers have detected glyphosate fluorescently with carbon and graphene dots, [ 39 , 40 , 41 , 42 ] electrochemically with carbon paste and screen printed electrodes functionalized with reduced graphene oxide, [ 43 , 44 , 45 , 46 , 47 ] and optically with a graphene/zinc oxide nanocomposite. [ 48 ] However, carbon dot synthesis typically requires various toxic chemical reagents as well as high thermal or electrical energy input; [ 49 ] graphene synthesis usually involves mechanical/chemical exfoliation, thermal decomposition of silicon carbide or chemical vapor deposition, [ 50 ] while screen, aerosol, gravure and inkjet printed graphene require ink formulation and post‐print annealing, masks or stencils, and aggressive solvents like N ‐methyl‐2‐pyrrolidone (NMP) and N , N ‐dimethyl formamide (DMF) or even low boiling point solvents that suffer from poor graphene dispersion.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Laser‐Induced Graphene Biosensor for Electrochemical Sensing of the Herbicide Glyphosate

et al. 2022

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synchronization with the adoption of genetically modified crops that possess glyphosate resistance. [1] Glyphosate maintains the largest share of herbicide use with an application of 113.4 million kg in the United States and 747 million kg globally, as reported in 2014. [2] Although glyphosate is largely believed to be nontoxic to animals and humans, its accumulation in ground water after heavy rains and movement into surface waters has increased concern about its larger environmental and human health impact. [3] Notably, glyphosate has been detected in concentrations up to 27.8 µg L −1 in 44% of Midwestern stream samples from a study performed during the 2013 Midwestern growing season. [4] A South Carolina and Minnesota study showed positive glyphosate detection in farmers' urine during their application cycle of up to 3.2 µg L −1 , [5] while a similar study in Wisconsin confirmed a maximum sample concentration of 12 µg kg −1 . [6] Perhaps more worrisome, the International Agency for Research on Cancer (IARC) classified glyphosate as a "probable human carcinogen," a claim highly contested by the Environmental Protection Agency (EPA) and European Food Safety Authority (EFSA), [7] though critiques have been made regarding both the EPA's and European Food Safety Authority's analysis Glyphosate is a globally applied herbicide yet it has been relatively undetectable in-field samples outside of gold-standard techniques. Its presumed nontoxicity toward humans has been contested by the International Agency for Research on Cancer, while it has been detected in farmers' urine, surface waters and crop residues. Rapid, on-site detection of glyphosate is hindered by lack of field-deployable and easy-to-use sensors that circumvent sample transportation to limited laboratories that possess the equipment needed for detection. Herein, the flavoenzyme, glycine oxidase, immobilized on platinumdecorated laser-induced graphene (LIG) is used for selective detection of glyphosate as it is a substrate for GlyOx. The LIG platform provides a scaffold for enzyme attachment while maintaining the electronic and surface properties of graphene. The sensor exhibits a linear range of 10-260 µm, detection limit of 3.03 µm, and sensitivity of 0.991 nA µm −1 . The sensor shows minimal interference from the commonly used herbicides and insecticides: atrazine, 2,4-dichlorophenoxyacetic acid, dicamba, parathion-methyl, paraoxon-methyl, malathion, chlorpyrifos, thiamethoxam, clothianidin, and imidacloprid. Sensor function is further tested in complex river water and crop residue fluids, which validate this platform as a scalable, direct-write, and selective method of glyphosate detection for herbicide mapping and food analysis.

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Enzymatic electrochemical biosensor for glyphosate detection based on acid phosphatase inhibition

Cited by 17 publications

References 40 publications

Recent Advances in Nanoparticle-Based Optical Sensors for Detection of Pesticide Residues in Soil

Recent Advances in Nanoparticle-Based Optical Sensors for Detection of Pesticide Residues in Soil

A Non-Enzymatic Electrochemical Sensor for Glyphosate Adopting Surface Modified Screen-Printed Electrodes

Enzymatic Laser‐Induced Graphene Biosensor for Electrochemical Sensing of the Herbicide Glyphosate

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