Enzymatic determination of urinary citrate based on flow injection system using NUV spectroscopy and PLS regression

Sun, Qiyong; Tu, Jiawei; Yaroshenko, Irina; Kirsanov, Dmitry; Legin, Andrey; Wang, Ping

doi:10.1016/j.snb.2017.04.027

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Several analytical methods have been based on kinetic systems applying enzymes in solution and immobilized in areas such as clinical biochemistry [1][2][3][4][5]. Some of the characteristics inherent to developing analytical determinations based on the use of enzymes are sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system

et al. 2023

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A stopped-flow microfluidic system to monitor glutathione peroxidase (GPx) activity and evaluate potential inhibitors of the enzyme has been developed based on the integration of the microfluidic chip in the reaction/detection zone. This integration supposes the physical alignment at the optimal location of the microfluidic channel, both the magnetically retained enzyme microreactor (MREµR) and the remote luminescence detection using a focused bifurcated fiber optic bundle (BFOB) connected to a conventional spectrofluorometer detector. The method is based on the coupling of two competitive oxidative chemical reactions, in which glutathione (GSH) and homovanillic acid (HVA) competed for their interaction with hydrogen peroxide in the presence of the magnetically retained GPx-MNPs. The biocatalytic reaction was followed by monitoring the fluorescence of the biphenyl-HVA dimer formed. The dynamic range of the calibration graph was 0.45–10 µmol L−1, expressed as GSH concentration with a detection limit of 0.1 µmol L−1 (r2 = 0.9954, n = 10, r = 3). The precision expressed as the relative standard deviation (RSD%) was between 0.5 and 3.9%. The stopped-flow microfluidic system showed a sampling frequency of 25 h−1. The method was applied to the study of GPx inhibition provided by three inhibitory compounds, two metallic ions Hg(II) and Cu(II) and t-butyl hydroperoxide, and their presence in liquid samples, as water, milk, and edible oil. Recovery values between 88.7 and 99.4% were achieved in all instances. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system

et al. 2023

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Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

et al. 2020

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In this review, we cite references from the period between 2015 and 2020 related to the use of a flow-based system as a tool to obtain a modern analytical system for speeding up data production and improving performance. Based on a great deal of concepts for automatic systems, there are several research groups introduced in the development of flow-based systems to increase sample throughput while retaining the reproducibility and repeatability as well as to propose new platforms of flow-based systems, such as microfluidic chip and paper-based devices. Additionally, to apply a developed system for on-site analysis is one of the key features for development. We believe that this review will be very interested and useful for readers because of its impact on developing novel analytical systems. The content of the review is categorized following their applications including quality control and food safety, clinical diagnostics, environmental monitoring and miscellaneous.

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部分的最小二乗法によるフローインジェクション分析の迅速化—フェナントロリン吸光光度法を用いる鉄の定量による検証—

et al. 2021

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Enzymatic determination of urinary citrate based on flow injection system using NUV spectroscopy and PLS regression

Cited by 3 publications

References 36 publications

Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system

Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

部分的最小二乗法によるフローインジェクション分析の迅速化—フェナントロリン吸光光度法を用いる鉄の定量による検証—

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