Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts

Carr, Timothy P.; Andresen, Catharine J.; Rudel, Lawrence L.

doi:10.1016/0009-9120(93)90015-x

Cited by 501 publications

(305 citation statements)

References 8 publications

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“…as described [32]. Triacylglycerol was then quantified spectrophotometrically using an enzymatic assay kit (GPO-PAP; Horiba-ABX, Montpellier, France).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Il6 leads to hyperinsulinaemia, liver inflammation and reduced body weight in mice

et al. 2008

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Aims/hypothesis IL-6 is released by the adipose tissue and increased circulating levels in obesity are associated with hyperinsulinaemia and insulin resistance. Short-term experiments suggest that increased IL-6 release by the skeletal muscle following exercise may improve insulin sensitivity. Methods In order to examine the effect of chronically elevated IL-6 levels, we overexpressed Il6 in skeletal muscle in mice using an electro-transfer procedure.Results Circulating IL-6 levels were increased and the animals rapidly lost both weight and body fat, but food intake was unchanged, which is consistent with the finding that IL-6 increased energy expenditure. Insulin levels were inappropriately elevated and combined with hypoglycaemia in spite of reduced 2-deoxy-D-glucose uptake by skeletal muscle. Insulin-stimulated glucose uptake by skeletal muscles ex vivo was reduced, probably due to the decreased amounts of glucose transporter (GLUT)-4. Beta cell insulin content was increased, while apparent beta cell mass was unchanged. Circulating serum amyloid A cluster levels were increased tenfold due to a pronounced proinflammatory state in the liver with infiltration of inflammatory cells. However, no liver steatosis was found, which may be accounted for by concomitant AMP kinase activation. Conclusions/interpretation Chronically elevated IL-6 levels lead to inappropriate hyperinsulinaemia, reduced body weight, impaired insulin-stimulated glucose uptake by the skeletal muscles and marked inflammation in the liver. Thus, the pleiotrophic effects of chronically elevated IL-6 levels preclude any obvious usefulness in treating obesity or its associated metabolic complications in man, despite the fact that weight reduction may be expected.

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“…as described [32]. Triacylglycerol was then quantified spectrophotometrically using an enzymatic assay kit (GPO-PAP; Horiba-ABX, Montpellier, France).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Il6 leads to hyperinsulinaemia, liver inflammation and reduced body weight in mice

et al. 2008

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“…Plasma total cholesterol concentration was measured enzymatically as previously described [25] using regents purchased from Roche Diagnostics (Indianapolis, Ind, USA). The HDL cholesterol quantification was performed using the total cholesterol assay after precipitation of apolipoprotein-B-containing lipoproteins (Thermo Electron Corp, Melbourne, Australia).…”

Section: Plasma Lipid Analysesmentioning

confidence: 99%

“…Triton X-100 (10% in chloroform; vol/vol) was added to liver lipid extracts to solubilize the lipids as previously described [25]. Quantification of total cholesterol, free cholesterol, phospholipids, and triglycerides were performed in duplicate.…”

Section: Liver Lipid Quantificationsmentioning

confidence: 99%

Phytosterol stearate esters elicit similar responses on plasma lipids and cholesterol absorption but different responses on fecal neutral sterol excretion and hepatic free cholesterol in male Syrian hamsters

et al. 2011

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“…Plasma concentrations of TC and triglyceride (TG) were determined using commercially available reagents (cholesterol/HP and triglyceride/GB; Roche Diagnostics, Indianapolis, IN, USA) with an enzymatic colorimetric method previously adapted for microtiter plates (Carr et al, 1993). Cholesterol standards were prepared as dilutions of a stock solution (Cholesterol Calibrator 200 mg/dl; Sigma Chemical, St Louis, MO, USA) and TG standards were created from a stock solution of oil with t-octylphenoxypolyethoxyethanol added for solubility (Triton X-100, Sigma Chemical, St Louis, MO, USA).…”

Section: Sample Analysismentioning

confidence: 99%

Probiotic capsules do not lower plasma lipids in young women and men

et al. 2007

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Objective: To investigate the effect of probiotic capsules on plasma lipids. Design: A randomized, single-blinded, placebo-controlled, parallel-arm trial. Subjects: Fifty-five normocholesterolemic subjects ages 18-36 (33 premenopausal women and 22 men). Intervention: Each subject consumed either three probiotic capsules each containing a total of 10 9 colony-forming units Lactobacillus acidophilus and Bifidobacterium longum and 10-15 mg fructo-oligosaccharide or three placebo capsules daily for 2 months (men) or two menstrual cycles (women). Plasma lipids were measured before and following the intervention (during the early follicular phase for women). Results: Plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglyceride were not altered by consumption of probiotic or placebo capsules and were not different between treatment groups following the intervention. Conclusions: These results do not support a beneficial effect of Lactobacillus acidophilus strain DDS-1 and Bifidobacterium longum strain UABL-14 on plasma lipids in normocholesterolemic young women and men. Sponsorship: Supported by the Minnesota Agricultural Experiment Station and UAS Laboratories.

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Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts

Cited by 501 publications

References 8 publications

Overexpression of Il6 leads to hyperinsulinaemia, liver inflammation and reduced body weight in mice

Overexpression of Il6 leads to hyperinsulinaemia, liver inflammation and reduced body weight in mice

Phytosterol stearate esters elicit similar responses on plasma lipids and cholesterol absorption but different responses on fecal neutral sterol excretion and hepatic free cholesterol in male Syrian hamsters

Probiotic capsules do not lower plasma lipids in young women and men

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