Enzymatic determination of glucose in culture liquor with glucose oxidase for use in food

Khalyutkin, A. N.; Prokopenko, E. I.; Itsygin, S. B.; Карпова, О. В.; Kovaleva, T. A.; Semenova, I.A.

doi:10.1007/bf00770458

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“…[9][10][11] Its properties and structure are still the motivation for a great number of studies and a thorough description can be found in the literature. [12][13][14][15][16][17][18][19] The adsorption of GOx at both the ITIES and the air/ water interface in the absence of a lipid monolayer has been shown to be dependent on time, GOx concentration, and ionic strength.…”

Section: Introductionmentioning

confidence: 99%

Adsorption–Penetration Studies of Glucose Oxidase into Phospholipid Monolayers at the 1,2‐Dichloroethane/Water Interface

Santos¹,

Ferreira²,

Pereira³

et al. 2007

ChemPhysChem

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The interaction between glucose oxidase (GOx) and phospholipid monolayers is studied at the 1,2-dichloroethane/water interface by electrochemical impedance spectroscopy. Electrochemical experiments show that the presence of GOx induces changes in the capacitance curves at both negative and positive potentials, which are successfully explained by a theoretical model based on the solution of the Poisson-Boltzmann equation. These changes are ascribed to a reduced partition coefficient of GOx and an increase of the permittivity of the lipid hydrocarbon domain. Our results show that the presence of lipid molecules enhances the adsorption of GOx molecules at the liquid/liquid interface. At low lipid concentrations, the adsorption of GOx is probably the first step preceding its penetration into the lipid monolayer. The experimental results indicate that GOx penetrates better and forms more stable monolayers for lipids with longer hydrophobic tails. At high GOx concentrations, the formation of multilayers is observed. The phenomenon described here is strongly dependent on 1) the GOx and lipid concentrations, 2) the nature of the lipid, and 3) the potential drop across the interface.

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