Enzymatic determination of bilirubin fractions in serum

Kosaka, Atsuko; Yamamoto, Chieko; Morishita, Yoshiaki; Nakane, Kiyoshi

doi:10.1016/0009-9120(87)90014-2

Cited by 44 publications

(29 citation statements)

References 17 publications

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“…Copper acts here as a cofactor of the enzyme bilirubin oxidase, which is known to belong to the group of copper-based enzymes [5]. Copper effect on the performance of the bilirubin biosensor was tested in the concentration range 1.0610…”

Section: Working Conditionsmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

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An enzymic method for the determination of bilirubin is described, based on the indirect electrochemical monitoring of bilirubin via its reaction with oxygen to biliverdin in the presence of bilirubin oxidase. Amperometric signals due to oxygen depletion were taken by means of an oxygen electrode incorporating an immobilized bilirubin oxidase membrane. The calibration plot shows linearity up to 2.0610 74 M. This concentration range corresponds to elevated bilirubin concentrations in adults and in newborn infants. The RSD of the method is 3.1 % (1.0610 74 M bilirubin, n 6). The method is interference free, fast (`3min) and easy to perform. The membrane retains almost 80 % of its initial activity after 30 successive measurements.

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Section: Working Conditionsmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

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“…Previously, it has been found that indirect bilirubin (I-Bil) corresponds to unconjugated bilirubin (Bu), and D-Bil, to conjugated bilirubin (Bc) (1). However, Bu and Bδ react in small amounts with diazo reagent in direct assay, and some observations have been also reported with other methods such as the BOD and vanadate methods (1)(2)(3)(4). In terms of clinical significance, values of Bc and Bδ are better indicators of liver function than are the values of D-Bil, T-Bil, and hepatic enzymes such as AST and ALP (1,(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 95%

Enzymatic assay for conjugated bilirubin (Bc) in serum using bilirubin oxidase (BOD)

Kimura

Iyama

Yamaguchi

et al. 1999

J. Clin. Lab. Anal.

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“…3A). M. verrucaria BOD also reportedly oxidizes ditaurobilirubin at acidic pH, which proved useful for selective measurement of conjugated bilirubin at about pH 5.5 (10). Thus, the large difference in the optimum pHs for CotA-catalyzed oxidation of bilirubin and ditaurobilirubin may be advantageous for selective determination of conjugated bilirubin levels in serum.…”

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confidence: 97%