Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Pitschmann, Vladimír; Matějovský, Lukáš; Vetchý, David; Kobliha, Zbyněk

doi:10.1080/00032719.2016.1151889

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…Comparing with the natural polymers, the synthetic polymers are more systemically studied, and the most representative is the macromolecule epoxy series . To improve the property of immobilized PGA, some reports have designed a large number of novel composite carriers based on advantages from both the organic materials and the inorganic materials …”

Section: Introductionmentioning

confidence: 99%

“…13,14 To improve the property of immobilized PGA, some reports have designed a large number of novel composite carriers based on advantages from both the organic materials and the inorganic materials. 15,16 Although there are many studies on PGA immobilization, the compatibility of PGA carrier in terms of high load, fast response rate, and high recovery is not well resolved. Because the enzyme loading is positively correlated with the specific surface area of the carrier, the main means to increase the loading is to reduce the particle size of the carrier or employ the porous carrier, 17 by following problems occurre, which are mainly about the compatibility of PGA loading and recovery rate of carrier due to the surface area and size of the carrier.…”

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confidence: 99%

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Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Chen

Liu

et al. 2018

Polymers for Advanced Techs

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Reversible‐addition‐fragmentation chain transfer polymerization method was adopted here in this study to prepare a series of thermo‐sensitive tri‐block copolymer carrier, poly(N,N‐diethylacrylamide‐b‐β‐hydroxyethyl methacrylate‐b‐glycidyl methacrylate), PDEA‐b‐PHEMA‐b‐PGMA (named as DHG). Their structures and temperature sensitivity performances were further characterized by the nuclear magnetic resonance, the gel permeation chromatography, and the fluorescence spectroscopy, respectively. It has been identified that the synthesis reaction of tri‐block copolymer was living polymerization. The thermo‐sensitivity study suggested that 2 monomers, β‐hydroxyethyl methacrylate and glycidyl methacrylate, played key roles on the lower critical solution temperature performance. Finally, above block copolymer was conscripted to immobilize penicillin G acylase; relationships among the number‐average molar weight ()trueM¯n of the 2 monomer, β‐hydroxyethyl methacrylate and glycidyl methacrylate, loading (L), and activity recover rate (Ar) of immobilized PGA were investigated. And the results showed that L and Ar arrived at 19 502 U and 92%, respectively.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Chen

Liu

et al. 2018

Polymers for Advanced Techs

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“…As far as interfering substances are concerned, they are well known and the presence of buffers in the detection tubes makes the detection itself robust to acids and bases 42 . As for the ions commonly found in surface water, it has already been confirmed that they have a negligible effect on Δ E 2000 values in the case of activity and inhibition test 26 …”

Section: Resultsmentioning

confidence: 99%

The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents

Zeman

Pavloková

Vetchý

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Maintaining the stability of an immobilized enzyme during storage is generally very problematic. However, various substances have been reported to have a positive effect on enzyme stability. These include reducing disaccharide lactose, which is used in carriers in the form of pellets with immobilized cholinesterase. These carriers serve as a filling for detection tubes (biosensor), which are used for detecting nerve agents, wherein the detection itself is based on a colorimetric Ellman's reaction. To date, however, no study has been performed on the effect of different types of lactose monohydrate on the properties of such carriers. RESULTS The effect of five different types of lactose monohydrate on carrier properties and enzyme stability was examined. The lactose powders used differed in the particle size distribution, shape, compactability and flowability. The carriers were prepared in triplicate and subjected to a 24‐month stability test under three storage conditions, with the results being statistically analyzed by analysis of variance, principal component analysis and t‐test. Samples containing lactose with spherical particles were more resistant to degradation by heat and moisture than samples containing crystalline particles; in addition they had higher hardness and better sensitivity. All samples were also tested with soman after 24 months of storage and were found to retain very good sensitivity. CONCLUSION Samples containing spherical types of lactose proved to be more stable in some critical parameters compared to the samples with lactose in the form of single crystals, which are currently used in commercially available detection tubes. © 2021 Society of Chemical Industry (SCI).

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“…Recently, however, acetylcholine and butyrylcholine have largely been replaced with acetylthiocholine or butyrylthiocholine, respectively, in which case thiocholine is produced instead of choline. Thiocholine changes the color of redox indicators, such as Ellman’s reagent [ 9 , 10 , 11 ], 2,6-dichlorophenolindophenol [ 12 ] or its analogues [ 13 ], or triphenylmethane dyes such as Guinea Green B and Malachite Green [ 4 , 14 , 15 ]. Chromogenic substrates, e.g., 2,6-dichlorophenolindophenyl acetate [ 16 ] and indoxyl acetate [ 17 ], which decompose directly on colored products, are frequently used alternatives.…”

Section: Introductionmentioning

confidence: 99%

Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

et al. 2018

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Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman’s reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%).

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Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Cited by 12 publications

References 12 publications

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents

Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

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