Enzymatic determination of acetazolamide in human plasma

Yakatan, Gerald J.; Martin, Carol A.; Smith, Robert V.

doi:10.1016/s0003-2670(01)82852-x

Cited by 14 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The blood concentrations of inhibitors, calculated from known infusion rates and venous blood flows, were checked by assay in one experiment with each inhibitor. Concentrations of acetazolamide and benzolamide in venous plasma and saliva samples were measured by an enzyme inhibitor assay similar to that described by Yakatan, Martin & Smith (1976). Purified ovine erythrocyte carbonic anhydrase was inhibited by known concentrations of acetazolamide or benzolamide and a standard curve (% inhibition V8.…”

Section: Methodsmentioning

confidence: 99%

The effect of carbonic anhydrase inhibitors on the anionic composition of sheep's parotid saliva. With an appendix on uncatalysed carbon dioxide‐water kinetics by P. T. McTigue.

Blair‐West¹,

Fernley²,

Nelson³

et al. 1980

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The effects of the carbonic anhydrase inhibitors, acetazolamide, ethoxzolamide and benzolamide on the ionic composition of parotid saliva were studied in anaesthetized sheep with access to the parotid blood vessels.2. The inhibitors were infused directly into the arterial blood supply to the gland to give blood concentrations in the range 10-5 to 10-2 M.3. Mean anionic concentrations at basal flow rate before inhibitor infusion were, bicarbonate 98 m-mole/l., phosphate 15 m-mole/l. and chloride 26 m-mole/l. In the presence of inhibitors, bicarbonate concentration fell by 11 m-mole/ml. and phosphate-and chloride concentrations rose. Secreto-motor nerve stimulation increased bicarbonate concentration by 13 m-mole/l. before infusion of inhibitors and the concentrations of the other anions fell. The bicarbonate rise was abolished by the inhibitors and the fall in phosphate concentration was balanced by a rise in chloride concentration.4. These effects show that only a small component of the bicarbonate ion transfer system in the sheep parotid gland is sensitive to these inhibitors.5. The relationship of these findings to a new enzyme with carbonic anhydrase action isolated from the sheep's parotid gland is discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

The effect of carbonic anhydrase inhibitors on the anionic composition of sheep's parotid saliva. With an appendix on uncatalysed carbon dioxide‐water kinetics by P. T. McTigue.

Blair‐West¹,

Fernley²,

Nelson³

et al. 1980

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…Since the decrease in the catalytic rate is proportional to the chlorthalidone concentration, measurement of this decrease can be used to determine the chlorthalidone level in unknown samples. This principle was utilized previously for the assay of acetazolamide (5,6) and furosemide (7). Carbonic anhydrase catalyzes the hydration of carbon dioxide, and the activity of the enzyme toward the physiological substrate, carbon dioxide, is usually measured by determining the rate of increase that the enzyme causes in pH during bubbling of carbon dioxide through a slightly alkaline buffer (5).…”

mentioning

confidence: 99%

Chlorthalidone Analysis Using Carbonic Anhydrase Inhibition

Johnston¹,

Li²,

Mufson³

1977

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Relative bioavailability of acetazolamide tablets

Straughn

Gollamudi

Meyer

1982

Biopharm & Drug Disp

View full text Add to dashboard Cite

Plasma acetazolamide concentrations were determined enzymatically after administration of three tablet dosage forms and a reference solution to 12 human subjects in a crossover study. Two of the tablet products represented different lots from the same manufacturer. There were no significant differences in area under the plasma level-time curves among the four treatments. However, significant differences were found among tablets in terms of peak plasma concentration and time to reach peak concentration. These apparent differences in rate of absorption were correlated with in vitro dissolution data obtained in pH 1.5 dissolution medium.

show abstract

Enzymatic determination of acetazolamide in human plasma

Cited by 14 publications

References 1 publication

The effect of carbonic anhydrase inhibitors on the anionic composition of sheep's parotid saliva. With an appendix on uncatalysed carbon dioxide‐water kinetics by P. T. McTigue.

The effect of carbonic anhydrase inhibitors on the anionic composition of sheep's parotid saliva. With an appendix on uncatalysed carbon dioxide‐water kinetics by P. T. McTigue.

Chlorthalidone Analysis Using Carbonic Anhydrase Inhibition

Relative bioavailability of acetazolamide tablets

Contact Info

Product

Resources

About