Several solutions are today proposed to farmers to minimize ammonia (NH 3 ) emissions during storage. In the present study, special attention was given to slurry acidification and slurry crust enhancement and our objective was to assess the effect of slurry bio-acidification using sugar and cheese whey as an alternative to sulphuric acid, and the potential of rice bran as crust enhancer on NH 3 and greenhouse gases emissions during storage. Both the cheese whey and the rice bran are materials, available in large amounts, with low commercial value in some EU regions as Portugal and its use, at farm scale, will be a win-win situation. Sugar is also a good alternative to acid attending its relatively low value. A laboratory experiment was performed for 2 months with five treatments: non-treated cattle slurry (CTRL), slurry treated with sulphuric acid (ACID), slurry treated with sugar (SUGAR), slurry treated with cheese whey (WHEY) and rice bran applied on the slurry surface (RICE). The SUGAR treatment led to a reduction of NH 3 emissions by 45% relative to CTRL while WHEY and RICE resulted in a reduction of 68% and 25%, respectively. Nevertheless, this effect of SUGAR and WHEY was shorter than in ACID, since NH 3 emissions started to be observed in those 2 treatments after 31 and 35 days of storage, respectively. Nitrous oxide emissions remained close to zero in ACID and SUGAR. RICE led to the highest emissions of carbon dioxide (CO 2 ) releasing almost 5% of carbon present in the initial mixture (slurry þ rice bran) and presented the highest methane emissions. The ACID and SUGAR led to a significant decrease of the total greenhouse gas (GHG) emissions. Our results indicate that bio-acidification using a source of sugar could be a good alternative to H 2 SO 4 to reduce simultaneously NH 3 and GHG emissions during storage.